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Original article
Published online: 2020-10-26
Submitted: 2020-09-18
Accepted: 2020-10-07
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Concomitant administration of sitagliptin and rutin improve the adverse hepatic alterations in streptozotocin-induced diabetes mellitus in albino rats, an overlook on the role of alpha smooth muscle actin

R. A. Attia, S. Abdel Fattah, M. M. Nasralla
DOI: 10.5603/FM.a2020.0130
·
Pubmed: 33124035

open access

Ahead of Print
ORIGINAL ARTICLES
Published online: 2020-10-26
Submitted: 2020-09-18
Accepted: 2020-10-07

Abstract

Background: Diabetes mellitus (DM), one of the commonest worldwide metabolic conditions, recognized to persuade oxidant/antioxidant discrepancies. Sitagliptin is an oral anti-hyperglycemic remedy that blocks dipeptidyl peptidase 4 (DPP4). Rutin is a polyphenolic natural flavonoid which owns antioxidant and anti-proliferative activity. The aim of the present work is to elucidate the concomitant effect of Sitagliptin and rutin on the deleterious alterations in the liver of experimentally induced diabetes in rats.

Materials and methods: 50 adult male albino rats, weighing 170-200 g were used. Rats were randomly divided into 5 groups (n=10).  Group 1 (control group), the other 4 groups (Groups II, III, IV and V) received a single i.p. injection of STZ, 65 mgKg-1 body weight to induce diabetes; group II (diabetic), group III (diabetic and rutin administered), group IV (diabetic and sitagliptin administered), and group V (diabetic with sitagliptin and rutin concomitantly administered).  H&E, masson trichrome, PAS, immune-histochemical; α-smooth muscle actin (α-SMA), histomorphometric analysis, liver enzymes and  oxidatants / anti-oxidatants; malondialdehyde (MDA)/ glutathione (GSH) and were done.

Results: Distorted hepatic architecture, dilatation, congestion of sinusoids and central veins as well as cytoplasmic vacuolations were remarkable changes in the diabetic group. There was extravasation of blood, diffuse fibrous tissue formation, increase in the mean values of liver enzymes, oxidative markers and α-SMA expression in the same group. The aforementioned changes were ameliorated in groups III and IV. Concomitant administration of sitagliptin and rutin resulted in marked enhancement of these hepatic alterations.

Conclusions: Combination of sitagliptin and rutin has an ameliorating effect on the hepatic deterioration induced by diabetes, which   is better than either sitagliptin or rutin alone.

Abstract

Background: Diabetes mellitus (DM), one of the commonest worldwide metabolic conditions, recognized to persuade oxidant/antioxidant discrepancies. Sitagliptin is an oral anti-hyperglycemic remedy that blocks dipeptidyl peptidase 4 (DPP4). Rutin is a polyphenolic natural flavonoid which owns antioxidant and anti-proliferative activity. The aim of the present work is to elucidate the concomitant effect of Sitagliptin and rutin on the deleterious alterations in the liver of experimentally induced diabetes in rats.

Materials and methods: 50 adult male albino rats, weighing 170-200 g were used. Rats were randomly divided into 5 groups (n=10).  Group 1 (control group), the other 4 groups (Groups II, III, IV and V) received a single i.p. injection of STZ, 65 mgKg-1 body weight to induce diabetes; group II (diabetic), group III (diabetic and rutin administered), group IV (diabetic and sitagliptin administered), and group V (diabetic with sitagliptin and rutin concomitantly administered).  H&E, masson trichrome, PAS, immune-histochemical; α-smooth muscle actin (α-SMA), histomorphometric analysis, liver enzymes and  oxidatants / anti-oxidatants; malondialdehyde (MDA)/ glutathione (GSH) and were done.

Results: Distorted hepatic architecture, dilatation, congestion of sinusoids and central veins as well as cytoplasmic vacuolations were remarkable changes in the diabetic group. There was extravasation of blood, diffuse fibrous tissue formation, increase in the mean values of liver enzymes, oxidative markers and α-SMA expression in the same group. The aforementioned changes were ameliorated in groups III and IV. Concomitant administration of sitagliptin and rutin resulted in marked enhancement of these hepatic alterations.

Conclusions: Combination of sitagliptin and rutin has an ameliorating effect on the hepatic deterioration induced by diabetes, which   is better than either sitagliptin or rutin alone.

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Keywords

streptozotocin, liver, diabetes, α-SMA, sitagliptin, rutin

About this article
Title

Concomitant administration of sitagliptin and rutin improve the adverse hepatic alterations in streptozotocin-induced diabetes mellitus in albino rats, an overlook on the role of alpha smooth muscle actin

Journal

Folia Morphologica

Issue

Ahead of Print

Article type

Original article

Published online

2020-10-26

DOI

10.5603/FM.a2020.0130

Pubmed

33124035

Keywords

streptozotocin
liver
diabetes
α-SMA
sitagliptin
rutin

Authors

R. A. Attia
S. Abdel Fattah
M. M. Nasralla

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