open access

Vol 79, No 4 (2020)
Original article
Submitted: 2019-11-06
Accepted: 2019-12-14
Published online: 2020-01-07
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Calvarial bone defects in ovariectomised rats treated with mesenchymal stem cells and demineralised freeze-dried bone allografts

E. T. Kadiroğlu1, M. E. Akbalık2, E. Karaöz3, B. E. Kanay4, A. Dağ5, M. A. Ketani6, E. G. Eroğlu7, E. Uysal8, M. C. Tuncer9
·
Pubmed: 31930468
·
Folia Morphol 2020;79(4):720-735.
Affiliations
  1. Department of Periodontology, Faculty of Dentistry, Dicle University, Diyarbakır, 21280 Turkey.
  2. Department of Histology and Embryology, Faculty of Veterinary Medicine, Dicle University, Diyarbakır, 21280, Turkey
  3. Department of Histology and Embryology, Faculty of Medicine, University of İstinye, Istanbul, 34010, Turkey
  4. Department of Surgery, Faculty of Veterinary Medicine, Dicle University, Diyarbakır, Turkey.
  5. Department of Periodontology, Faculty of Dentistry, Dicle University, Diyarbakır, 21280 Turkey.
  6. Department of Histology and Embryology, Faculty of Veterinary Medicine, Dicle University, Diyarbakır, 21280, Turkey
  7. Oral and Dental Health Center, Van, Turkey
  8. Diyarbakır Vocational School of Technical Science, Dicle University, Diyarbakır, 21280, Turkey.
  9. Department of Anatomy, Faculty of Medicine, University of Dicle, Diyarbakır, Türkiye

open access

Vol 79, No 4 (2020)
ORIGINAL ARTICLES
Submitted: 2019-11-06
Accepted: 2019-12-14
Published online: 2020-01-07

Abstract

Background: The aim of the study was to investigate the ability of a combination of bone marrow mesenchymal stem cells (BM-MSCs) with and without demineralised freeze-dried bone allografts (DFDBAs) to induce bone regeneration in
calvarial defects in ovariectomised rats.

Materials and methods: Critical size defects were filled with a combination of DFDBAs and BM-MSCs or BM-MSCs alone. Eight weeks after calvarial surgery, the rats were sacrificed. The samples were analysed histologically and immunohistochemically.

Results: No difference was observed in vascularisation between groups C1 (animals with cranial defect only, control group) and O1 (animals with cranial defect only, ovariectomy group). Intramembranous ossification was observed at a limited level in groups C2 (animals with cranial defect with MSCs, control group) and O2 (animals with cranial defect with MSCs, ovariectomy group) compared to C1 and O1. In group C3 (animals with DFDBAs with MSCs, control group), the fibrous structures of the matrix became compact as a result of a bone graft having been placed in the cavity, but in group O3 (animals with DFDBAs with MSCs, ovariectomy group), the fibrous tissue was poorly distributed between the bone grafts for the most parts.

Conclusions: We conclude that the insertion of BM-MSCs enhances bone healing; however, the DFDBA/BM-MSC combination has little effect on overcoming impaired bone formation in ovariectomised rats.

Abstract

Background: The aim of the study was to investigate the ability of a combination of bone marrow mesenchymal stem cells (BM-MSCs) with and without demineralised freeze-dried bone allografts (DFDBAs) to induce bone regeneration in
calvarial defects in ovariectomised rats.

Materials and methods: Critical size defects were filled with a combination of DFDBAs and BM-MSCs or BM-MSCs alone. Eight weeks after calvarial surgery, the rats were sacrificed. The samples were analysed histologically and immunohistochemically.

Results: No difference was observed in vascularisation between groups C1 (animals with cranial defect only, control group) and O1 (animals with cranial defect only, ovariectomy group). Intramembranous ossification was observed at a limited level in groups C2 (animals with cranial defect with MSCs, control group) and O2 (animals with cranial defect with MSCs, ovariectomy group) compared to C1 and O1. In group C3 (animals with DFDBAs with MSCs, control group), the fibrous structures of the matrix became compact as a result of a bone graft having been placed in the cavity, but in group O3 (animals with DFDBAs with MSCs, ovariectomy group), the fibrous tissue was poorly distributed between the bone grafts for the most parts.

Conclusions: We conclude that the insertion of BM-MSCs enhances bone healing; however, the DFDBA/BM-MSC combination has little effect on overcoming impaired bone formation in ovariectomised rats.

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Keywords

bone healing, bone marrow mesenchymal stem cells (BM-MSCs), demineralised freeze-dried bone allografts (DFDBAs), ovariectomy, calvarial defect

About this article
Title

Calvarial bone defects in ovariectomised rats treated with mesenchymal stem cells and demineralised freeze-dried bone allografts

Journal

Folia Morphologica

Issue

Vol 79, No 4 (2020)

Article type

Original article

Pages

720-735

Published online

2020-01-07

Page views

1310

Article views/downloads

1402

DOI

10.5603/FM.a2020.0001

Pubmed

31930468

Bibliographic record

Folia Morphol 2020;79(4):720-735.

Keywords

bone healing
bone marrow mesenchymal stem cells (BM-MSCs)
demineralised freeze-dried bone allografts (DFDBAs)
ovariectomy
calvarial defect

Authors

E. T. Kadiroğlu
M. E. Akbalık
E. Karaöz
B. E. Kanay
A. Dağ
M. A. Ketani
E. G. Eroğlu
E. Uysal
M. C. Tuncer

References (37)
  1. Agacayak S, Gulsun B, Ucan MC, et al. Effects of mesenchymal stem cells in critical size bone defect. Eur Rev Med Pharmacol Sci. 2012; 16(5): 679–686.
  2. Akbalik ME, Ketani MA. Expression of epidermal growth factor receptors and epidermal growth factor, amphiregulin and neuregulin in bovine uteroplacental tissues during gestation. Placenta. 2013; 34(12): 1232–1242.
  3. Akita S, Fukui M, Nakagawa H, et al. Cranial bone defect healing is accelerated by mesenchymal stem cells induced by coadministration of bone morphogenetic protein-2 and basic fibroblast growth factor. Wound Repair Regen. 2004; 12(2): 252–259.
  4. Alfotawei R, Naudi KB, Lappin D, et al. The use of TriCalcium Phosphate (TCP) and stem cells for the regeneration of osteoperiosteal critical-size mandibular bony defects, an in vitro and preclinical study. J Craniomaxillofac Surg. 2014; 42(6): 863–869.
  5. Bertolai R, Catelani C, Aversa A, et al. Bone graft and mesenchimal stem cells: clinical observations and histological analysis. Clin Cases Miner Bone Metab. 2015; 12(2): 183–187.
  6. Calciolari E, Mardas N, Dereka X, et al. The effect of experimental osteoporosis on bone regeneration: part 2, proteomics results. Clin Oral Implants Res. 2017; 28(9): e135–e145.
  7. Caplanis N, Lee MB, Zimmerman GJ, et al. Effect of allogeneic freeze-dried demineralized bone matrix on regeneration of alveolar bone and periodontal attachment in dogs. J Clin Periodontol. 1998; 25(10): 801–806.
  8. Gamie Z, Tran GT, Vyzas G, et al. Stem cells combined with bone graft substitutes in skeletal tissue engineering. Expert Opin Biol Ther. 2012; 12(6): 713–729.
  9. He YX, Zhang Ge, Pan XH, et al. Impaired bone healing pattern in mice with ovariectomy-induced osteoporosis: A drill-hole defect model. Bone. 2011; 48(6): 1388–1400.
  10. Im JY, Min WK, You C, et al. Bone regeneration of mouse critical-sized calvarial defects with human mesenchymal stem cells in scaffold. Lab Anim Res. 2013; 29(4): 196–203.
  11. Intini G, Andreana S, Buhite RJ, et al. A comparative analysis of bone formation induced by human demineralized freeze-dried bone and enamel matrix derivative in rat calvaria critical-size bone defects. J Periodontol. 2008; 79(7): 1217–1224.
  12. Juluri R, Prashanth E, Gopalakrishnan D, et al. Association of postmenopausal osteoporosis and periodontal disease: a double-blind case-control study. J Int Oral Health. 2015; 7(9): 119–123.
  13. Kandal S, Özmen S, Uygur S, et al. Effects of rat bone marrow-derived mesenchymal stem cells and demineralized bone matrix on cranial bone healing. Ann Plast Surg. 2016; 77(2): 249–254.
  14. Karaoz E, Aksoy A, Ayhan S, et al. Characterization of mesenchymal stem cells from rat bone marrow: ultrastructural properties, differentiation potential and immunophenotypic markers. Histochem Cell Biol. 2009; 132(5): 533–546.
  15. Koob S, Torio-Padron N, Stark GB, et al. Bone formation and neovascularization mediated by mesenchymal stem cells and endothelial cells in critical-sized calvarial defects. Tissue Eng Part A. 2011; 17(3-4): 311–321.
  16. Kurkalli BG, Gurevitch O, Sosnik A, et al. Repair of bone defect using bone marrow cells and demineralized bone matrix supplemented with polymeric materials. Curr Stem Cell Res Ther. 2010; 5(1): 49–56.
  17. Lu W, Ji K, Kirkham J, et al. Bone tissue engineering by using a combination of polymer/Bioglass composites with human adipose-derived stem cells. Cell Tissue Res. 2014; 356(1): 97–107.
  18. McCann RM, Colleary G, Geddis C, et al. Effect of osteoporosis on bone mineral density and fracture repair in a rat femoral fracture model. J Orthop Res. 2008; 26(3): 384–393.
  19. Miron RJ, Wei L, Yang S, et al. Effect of enamel matrix derivative on periodontal wound healing and regeneration in an osteoporotic model. J Periodontol. 2014; 85(11): 1603–1611.
  20. Mokbel N, Bou Serhal C, Matni G, et al. Healing patterns of critical size bony defects in rat following bone graft. Oral Maxillofac Surg. 2008; 12(2): 73–78.
  21. Namkung-Matthai H, Appleyard R, Jansen J, et al. Osteoporosis influences the early period of fracture healing in a rat osteoporotic model. Bone. 2001; 28(1): 80–86.
  22. Palomo L, Williams K, Thacker H. Periodontal healing and osteoporosis in postmenopausal women. Ann Gerontol Geriatric Res. 2016; 3(3): 1043.
  23. Pires-Oliveira DAA, Oliveira RF, Amadei SU, et al. Laser 904 nm action on bone repair in rats with osteoporosis. Osteoporos Int. 2010; 21(12): 2109–2114.
  24. Richa RY, Puranik MP, Shrivastava A, et al. Association between osteoporosis and periodontal disease among postmenopausal Indian women. J Investig Clin Dent. 2017; 8(3).
  25. Riggs BL. The mechanisms of estrogen regulation of bone resorption. J Clin Invest. 2000; 106(10): 1203–1204.
  26. Saad KAE, Abu-Shahba AG, El-Drieny EAE, et al. Evaluation of the role of autogenous bone-marrow-derived mesenchymal stem cell transplantation for the repair of mandibular bone defects in rabbits. J Craniomaxillofac Surg. 2015; 43(7): 1151–1160.
  27. Semyari H, Rajipour M, Sabetkish S, et al. Evaluating the bone regeneration in calvarial defect using osteoblasts differentiated from adipose-derived mesenchymal stem cells on three different scaffolds: an animal study. Cell Tissue Bank. 2016; 17(1): 69–83.
  28. Sethi AK, Kar IB, Mohanty T, et al. Use of plasma-enriched demineralized freeze-dried bone matrix in postsurgical jaw defects. Natl J Maxillofac Surg. 2018; 9(2): 174–183.
  29. Stockmann P, Park J, von Wilmowsky C, et al. Guided bone regeneration in pig calvarial bone defects using autologous mesenchymal stem/progenitor cells - a comparison of different tissue sources. J Craniomaxillofac Surg. 2012; 40(4): 310–320.
  30. Sukumar S, Drízhal I. Bone grafts in periodontal therapy. Acta Medica (Hradec Kralove). 2008; 51(4): 203–207.
  31. Tarantino U, Cerocchi I, Scialdoni A, et al. Bone healing and osteoporosis. Aging Clin Exp Res. 2011; 23(2 Suppl): 62–64.
  32. Tera de Mellod T, Nascimento RD, Prado RF, et al. Immunolocalization of markers for bone formation during guided bone regeneration in osteopenic rats. J Appl Oral Sci. 2014; 22(6): 541–553.
  33. Vahabi S, Amirizadeh N, Shokrgozar MA, et al. A comparison between the efficacy of Bio-Oss, hydroxyapatite tricalcium phosphate and combination of mesenchymal stem cells in inducing bone regeneration. Chang Gung Med J. 2012; 35(1): 28–37.
  34. Viña JA, El-Alami M, Gambini J, et al. Application of mesenchymal stem cells in bone regenerative procedures in oral implantology. A literature review. J Clin Exp Dent. 2014; 6(1): e60–e65.
  35. Wang ZX, Chen C, Zhou Q, et al. The treatment efficacy of bone tissue engineering strategy for repairing segmental bone defects under osteoporotic conditions. Tissue Eng Part A. 2015; 21(17-18): 2346–2355.
  36. Watanabe Y, Harada N, Sato K, et al. Stem cell therapy: is there a future for reconstruction of large bone defects? Injury. 2016; 47: S47–S51.
  37. Weitzmann MN, Pacifici R. Estrogen deficiency and bone loss: an inflammatory tale. J Clin Invest. 2006; 116(5): 1186–1194.

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