open access

Vol 82, No 3 (2023)
Original article
Submitted: 2022-04-08
Accepted: 2022-06-07
Published online: 2022-06-22
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Potential therapeutic role of microvesicles derived from mesenchymal stem cells and platelet-rich plasma in murine burn wound healing: scar regulation and antioxidant mechanism

R. A. Imam1, M. M. Amer2
·
Pubmed: 35754188
·
Folia Morphol 2023;82(3):656-667.
Affiliations
  1. Department of Anatomy and Embryology, Faculty of Medicine, Cairo University, Egypt
  2. Anatomy and Embryology Department, Faculty of Medicine, Ain Shams University, Egypt

open access

Vol 82, No 3 (2023)
ORIGINAL ARTICLES
Submitted: 2022-04-08
Accepted: 2022-06-07
Published online: 2022-06-22

Abstract

Background: Microvesicles (MVs) derived from mesenchymal stem cells exhibited
an emerging promising therapy in many animal model diseases. Post-burn scars
represent one of the significant challenges in wound healing processes. The present
study investigated the possible role of MVs derived from mesenchymal stem cells
vs. platelet-rich plasma (PRP) in murine burn wound healing.
Materials and methods: Wistar rats (n = 40) were assigned into four equal
groups (control, burn, burn + PRP, burn + MVs). Small-sized burns were induced,
morphologically followed for 3 weeks, then rats were sacrificed and skin lesions
were analysed biochemically and immunohistochemically.
Results: Both MVs and PRP modulated the burn healing process with better results
in the MVs group than in PRP. MVs significantly (p < 0.05) accelerated burn
wound size healing and dramatically modulated tissue interleukin (IL)-10, IL-6, and
hyaluronidase. Both MVs and PRP significantly downregulated gene expression of
miRNA203 and alpha smooth muscle actin and immunoblotting analysis of matrix
metalloproteinases 3 and transforming growth factor beta compared with the
burn group. The immune-staining intensity of tumour necrosis factor alpha was
dramatically reversed in the MVs group compared with the burn group, whereas
that of connective tissue growth factor, collagen I and III was significantly reduced
in both groups. The antioxidant Nrf2 immune-staining intensity had been dramatically
enhanced particularly in MVs.
Conclusions: Microvesicles derived from mesenchymal stem cells and PRP may
improve burn wound healing via regulating scar formation and antioxidant mechanism.

Abstract

Background: Microvesicles (MVs) derived from mesenchymal stem cells exhibited
an emerging promising therapy in many animal model diseases. Post-burn scars
represent one of the significant challenges in wound healing processes. The present
study investigated the possible role of MVs derived from mesenchymal stem cells
vs. platelet-rich plasma (PRP) in murine burn wound healing.
Materials and methods: Wistar rats (n = 40) were assigned into four equal
groups (control, burn, burn + PRP, burn + MVs). Small-sized burns were induced,
morphologically followed for 3 weeks, then rats were sacrificed and skin lesions
were analysed biochemically and immunohistochemically.
Results: Both MVs and PRP modulated the burn healing process with better results
in the MVs group than in PRP. MVs significantly (p < 0.05) accelerated burn
wound size healing and dramatically modulated tissue interleukin (IL)-10, IL-6, and
hyaluronidase. Both MVs and PRP significantly downregulated gene expression of
miRNA203 and alpha smooth muscle actin and immunoblotting analysis of matrix
metalloproteinases 3 and transforming growth factor beta compared with the
burn group. The immune-staining intensity of tumour necrosis factor alpha was
dramatically reversed in the MVs group compared with the burn group, whereas
that of connective tissue growth factor, collagen I and III was significantly reduced
in both groups. The antioxidant Nrf2 immune-staining intensity had been dramatically
enhanced particularly in MVs.
Conclusions: Microvesicles derived from mesenchymal stem cells and PRP may
improve burn wound healing via regulating scar formation and antioxidant mechanism.

Get Citation

Keywords

microvesicles, stem cells, platelet-rich plasma, burn, miRNA203, rats

About this article
Title

Potential therapeutic role of microvesicles derived from mesenchymal stem cells and platelet-rich plasma in murine burn wound healing: scar regulation and antioxidant mechanism

Journal

Folia Morphologica

Issue

Vol 82, No 3 (2023)

Article type

Original article

Pages

656-667

Published online

2022-06-22

Page views

1251

Article views/downloads

888

DOI

10.5603/FM.a2022.0060

Pubmed

35754188

Bibliographic record

Folia Morphol 2023;82(3):656-667.

Keywords

microvesicles
stem cells
platelet-rich plasma
burn
miRNA203
rats

Authors

R. A. Imam
M. M. Amer

References (23)
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