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ORIGINAL ARTICLES
Published online: 2020-05-25
Submitted: 2020-04-03
Accepted: 2020-05-03
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Effect of genistein and estradiol on the adrenal cortex of the ovariectomized adult female albino rats

H. D. Yassa, N. M. Safwat, R. M. Ahmed, M. Z. Fathy, H. L. Metry
DOI: 10.5603/FM.a2020.0056
·
Pubmed: 32459363

open access

Ahead of Print
ORIGINAL ARTICLES
Published online: 2020-05-25
Submitted: 2020-04-03
Accepted: 2020-05-03

Abstract

Genistein, a naturally occurring soy isoflavone, attracts interest as an effective and safe alternative to hormone replacement therapy for menopausal proplems. The aim of the current study was to compare between the effect of genistein and estradiol on the adrenal cortex of the ovariectomized adult female albino rats. Twenty rats were used in the current study and divided into 4 groups, five rats in each group; group 1 (control nonovariectomized), group 2 (ovariectomized), group 3 (ovariectomized + genistein) and group 4 (ovariectomized+ estradiol). The rats were sacrificed after 4 weeks. Both adrenal glands were removed for light microscope using Hx & E. stain, ultrastructural study and immunohistochemical examination using PCNA, caspase-3, and ER-β. Ovariectomized rats showed signs of degeneration in all zones of adrenal cortex. On the other hand, Treatment with genistein showed restoration of the adrenal cortex with less proliferative effect than estradiol. So, genistein can be used as effective therapy to decrease the symptoms of menopause without fear of cancer development.

Abstract

Genistein, a naturally occurring soy isoflavone, attracts interest as an effective and safe alternative to hormone replacement therapy for menopausal proplems. The aim of the current study was to compare between the effect of genistein and estradiol on the adrenal cortex of the ovariectomized adult female albino rats. Twenty rats were used in the current study and divided into 4 groups, five rats in each group; group 1 (control nonovariectomized), group 2 (ovariectomized), group 3 (ovariectomized + genistein) and group 4 (ovariectomized+ estradiol). The rats were sacrificed after 4 weeks. Both adrenal glands were removed for light microscope using Hx & E. stain, ultrastructural study and immunohistochemical examination using PCNA, caspase-3, and ER-β. Ovariectomized rats showed signs of degeneration in all zones of adrenal cortex. On the other hand, Treatment with genistein showed restoration of the adrenal cortex with less proliferative effect than estradiol. So, genistein can be used as effective therapy to decrease the symptoms of menopause without fear of cancer development.

Get Citation

Keywords

adrenal, menopause, genistein, estradiol, rat

About this article
Title

Effect of genistein and estradiol on the adrenal cortex of the ovariectomized adult female albino rats

Journal

Folia Morphologica

Issue

Ahead of Print

Published online

2020-05-25

DOI

10.5603/FM.a2020.0056

Pubmed

32459363

Keywords

adrenal
menopause
genistein
estradiol
rat

Authors

H. D. Yassa
N. M. Safwat
R. M. Ahmed
M. Z. Fathy
H. L. Metry

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