open access

Ahead of Print
Original article
Published online: 2020-05-25
Submitted: 2020-04-03
Accepted: 2020-05-03
Get Citation

Effect of genistein and estradiol on the adrenal cortex of the ovariectomized adult female albino rats

H. D. Yassa, N. M. Safwat, R. M. Ahmed, M. Z. Fathy, H. L. Metry
DOI: 10.5603/FM.a2020.0056
·
Pubmed: 32459363

open access

Ahead of Print
ORIGINAL ARTICLES
Published online: 2020-05-25
Submitted: 2020-04-03
Accepted: 2020-05-03

Abstract

Genistein, a naturally occurring soy isoflavone, attracts interest as an effective and safe alternative to hormone replacement therapy for menopausal proplems. The aim of the current study was to compare between the effect of genistein and estradiol on the adrenal cortex of the ovariectomized adult female albino rats. Twenty rats were used in the current study and divided into 4 groups, five rats in each group; group 1 (control nonovariectomized), group 2 (ovariectomized), group 3 (ovariectomized + genistein) and group 4 (ovariectomized+ estradiol). The rats were sacrificed after 4 weeks. Both adrenal glands were removed for light microscope using Hx & E. stain, ultrastructural study and immunohistochemical examination using PCNA, caspase-3, and ER-β. Ovariectomized rats showed signs of degeneration in all zones of adrenal cortex. On the other hand, Treatment with genistein showed restoration of the adrenal cortex with less proliferative effect than estradiol. So, genistein can be used as effective therapy to decrease the symptoms of menopause without fear of cancer development.

Abstract

Genistein, a naturally occurring soy isoflavone, attracts interest as an effective and safe alternative to hormone replacement therapy for menopausal proplems. The aim of the current study was to compare between the effect of genistein and estradiol on the adrenal cortex of the ovariectomized adult female albino rats. Twenty rats were used in the current study and divided into 4 groups, five rats in each group; group 1 (control nonovariectomized), group 2 (ovariectomized), group 3 (ovariectomized + genistein) and group 4 (ovariectomized+ estradiol). The rats were sacrificed after 4 weeks. Both adrenal glands were removed for light microscope using Hx & E. stain, ultrastructural study and immunohistochemical examination using PCNA, caspase-3, and ER-β. Ovariectomized rats showed signs of degeneration in all zones of adrenal cortex. On the other hand, Treatment with genistein showed restoration of the adrenal cortex with less proliferative effect than estradiol. So, genistein can be used as effective therapy to decrease the symptoms of menopause without fear of cancer development.

Get Citation

Keywords

adrenal, menopause, genistein, estradiol, rat

About this article
Title

Effect of genistein and estradiol on the adrenal cortex of the ovariectomized adult female albino rats

Journal

Folia Morphologica

Issue

Ahead of Print

Article type

Original article

Published online

2020-05-25

DOI

10.5603/FM.a2020.0056

Pubmed

32459363

Keywords

adrenal
menopause
genistein
estradiol
rat

Authors

H. D. Yassa
N. M. Safwat
R. M. Ahmed
M. Z. Fathy
H. L. Metry

References (32)
  1. Almeida H, Magalhães MC, Magalhães MM. Age-related changes in lipid peroxidation products in rat adrenal gland. Age (Omaha). 1998; 21(3): 119–121.
  2. Bancroft JD, Gamble M. Theory and practice of histological techniques. 6th ed. Churchill Livingstone Elsevier, China 2008: 433–472.
  3. Bancroft JD, Cook HC. Immunocytochemistry. In: Manual of Histological Techniques and Diagnostic Applications. 1st ed., Churchill Livingstone, Edinburgh, London, Madrid, Melbourne, New York, Tokyo 1994: 263–325.
  4. Bozzola J, Russell L. Electron microscopy principles and techniques for biologists:. Jones and Bartlitt publishers 20 park plasa, Boston Mao 1991: 2116.
  5. Budhathoki S, Iwasaki M, Sawada N, et al. Soy food and isoflavone intake and endometrial cancer risk: the Japan Public Health Center-based prospective study. BJOG. 2015; 122(3): 304–311.
  6. Chang SP, Mullins JJ, Morley SD, et al. Transition from organogenesis to stem cell maintenance in the mouse adrenal cortex. Organogenesis. 2011; 7(4): 267–280.
  7. Darci Marinho S, Calió ML, Santos MA, et al. Evaluation of the isoflavones and estrogen effects on the rat adrenal. Gynecol Endocrinol. 2017; 33(10): 811–815.
  8. Drury R, Wallington E. Carleton's Histological techniques. 5th edition. Oxford 1980: 183–185.
  9. Eroschenko VP. DiFiore's atlas of histology with functional correlations. 12th edition. Lippincott Williams and Wilkins, Philadelphia 2013: 402–407.
  10. Gaete L, Tchernitchin AN, Bustamante R, et al. Daidzein-estrogen interaction in the rat uterus and its effect on human breast cancer cell growth. J Med Food. 2012; 15(12): 1081–1090.
  11. Hart KA, Barton MH. Adrenocortical insufficiency in horses and foals. Vet Clin North Am Equine Pract. 2011; 27(1): 19–34.
  12. Hirst JJ, West NB, Brenner RM, et al. Steroid hormone receptors in the adrenal glands of fetal and adult rhesus monkeys. J Clin Endocrinol Metab. 1992; 75(1): 308–314.
  13. Hyder SM, Huang JC, Nawaz Z, et al. Regulation of vascular endothelial growth factor expression by estrogens and progestins. Environ Health Perspect. 2000; 108 Suppl 5: 785–790.
  14. Jarzabek K, Koda M, Walentowicz-Sadlecka M, et al. Altered expression of ERs, aromatase, and COX2 connected to estrogen action in type 1 endometrial cancer biology. Tumour Biol. 2013; 34(6): 4007–4016.
  15. Kuiper GG, Carlsson B, Grandien K, et al. Comparison of the ligand binding specificity and transcript tissue distribution of estrogen receptors alpha and beta. Endocrinology. 1997; 138(3): 863–870.
  16. Laast VA, Larsen T, Allison N, et al. Distinguishing cystic degeneration from other aging lesions in the adrenal cortex of Sprague-Dawley rats. Toxicol Pathol. 2014; 42(5): 823–829.
  17. Liu J, Yuan F, Gao J, et al. Oral isoflavone supplementation on endometrial thickness: a meta-analysis of randomized placebo-controlled trials. Oncotarget. 2016; 7(14): 17369–17379.
  18. Lo MJ, Chang LL, Wang P. Effects of estradiol on corticosterone secretion in ovariectomized rats. J Cell Biochem. 2000; 77(4): 560–568, doi: 10.1002/(sici)1097-4644(20000615)77:4<560::aid-jcb4>3.0.co;2-d.
  19. Manson JE, Bassuk SS. The menopause transition and postmenopausal hormone therapy. In: Fauci AS, Braunwald E, Kasper DL, eds. Harrison’s Principles of Internal Medicine, 17th ed. McGrawHill, New York 2008: 2334–2339.
  20. Mescher AL. Adrenal Glands in: Junqueira's Basic Histology: Text and Atlas. 13th ed. Mcgraw-Hill Medical, New York 2013: 414–417.
  21. Nagata C, Mizoue T, Tanaka K, et al. Soy intake and breast cancer risk: an evaluation based on a systematic review of epidemiologic evidence among the Japanese population. Jpn J Clin Oncol. 2014; 44(3): 282–295.
  22. Parhizkar S, Abdul Latiff L, Parsa A. Effect of Nigella sativa on reproductive system in experimental menopause rat model. Avicenna J Phytomed. 2016; 6(1): 95–103.
  23. Rebuffat P, Belloni AS, Rocco S, et al. The effects of ageing on the morphology and function of the zonae fasciculata and reticularis of the rat adrenal cortex. Cell Tissue Res. 1992; 270(2): 265–272.
  24. Sabry MM, Elkalawy SAE, Abo-Elnour RKD, et al. Histolgical and immunohistochemical study on the effect of stem cell therapy on bleomycin induced pulmonary fibrosis in albino rat. Int J Stem Cells. 2014; 7(1): 33–42.
  25. Saruhan BG, Ozdemir N. Effect of ovariectomy and of estrogen treatment on the adrenal gland and body weight in rats. Saudi Med J. 2005; 26(11): 1705–1709.
  26. Seko K, Kagami H, Senga K, et al. Effects of ovariectomy and estrogen replacement on rat oral mucosa. Maturitas. 2005; 50(1): 44–51.
  27. Suckow M, Weisbroth S, Franklin C. The laboratory Rat, 2nd edition . American Colleage of Laboratory Animal Medicine, Academic Press, Toronto 2005.
  28. Teng J, Wang ZY, Jarrard DF, et al. Roles of estrogen receptor alpha and beta in modulating urothelial cell proliferation. Endocr Relat Cancer. 2008; 15(1): 351–364.
  29. Vinicius Carvalho DC, Silveira VÁ, do Prado RF, et al. Effect of estrogen therapy, soy isoflavones, and the combination therapy on the submandibular gland of ovariectomized rats. Pathol Res Pract. 2011; 207(5): 300–305.
  30. Ward JM, Reznik-Schüller H. Morphological and histochemical characteristics of pigments in aging F344 rats. Vet Pathol. 1980; 17(6): 678–685.
  31. Yang W, Wang S, Li Li, et al. Genistein reduces hyperglycemia and islet cell loss in a high-dosage manner in rats with alloxan-induced pancreatic damage. Pancreas. 2011; 40(3): 396–402.
  32. Young B, Woodford P, O'Dowd G. Adrenal Gland. In: Wheater's functional histology: a text and colour atlas. Sixth edition. Elsevier Health Sciences, Philadilphia 2013: 328–331.

Important: This website uses cookies. More >>

The cookies allow us to identify your computer and find out details about your last visit. They remembering whether you've visited the site before, so that you remain logged in - or to help us work out how many new website visitors we get each month. Most internet browsers accept cookies automatically, but you can change the settings of your browser to erase cookies or prevent automatic acceptance if you prefer.

By  "Via Medica sp. z o.o." sp.k., Świętokrzyska 73, 80–180 Gdańsk, Poland

tel.:+48 58 320 94 94, faks:+48 58 320 94 60, e-mail:  viamedica@viamedica.pl