open access

Vol 80, No 2 (2021)
Original article
Submitted: 2020-04-03
Accepted: 2020-05-03
Published online: 2020-05-25
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Effect of genistein and oestradiol on the adrenal cortex of the ovariectomised adult female albino rats

H. D. Yassa1, N. M. Safwat2, R. M. Ahmed1, M. Z. Fathy3, H. L. Metry4
·
Pubmed: 32459363
·
Folia Morphol 2021;80(2):380-391.
Affiliations
  1. Department of Anatomy and Embryology, Faculty of Medicine, Beni-Suef University, Beni-Suef, Egypt
  2. Department of Pathology, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef, Egypt
  3. Department of Surgery, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef, Egypt
  4. Department of Anatomy and Embryology, Faculty of Medicine, Cairo University, Cairo, Egypt

open access

Vol 80, No 2 (2021)
ORIGINAL ARTICLES
Submitted: 2020-04-03
Accepted: 2020-05-03
Published online: 2020-05-25

Abstract

Background: Genistein, a naturally occurring soy isoflavone, attracts interest as an effective and safe alternative to hormone replacement therapy for menopausal problems. The aim of the current study was to compare between the effect of genistein and oestradiol on the adrenal cortex of the ovariectomised adult female albino rats. Materials and methods: Twenty rats were used in the current study and divided into four groups, 5 rats in each group; group 1 (control non-ovariectomised), group 2 (ovariectomised), group 3 (ovariectomised + genistein) and group 4 (ovariectomised + oestradiol). The rats were sacrificed after 4 weeks. Both adrenal glands were removed for light microscope using haematoxylin and eosin stain, ultrastructural study and immunohistochemical examination using proliferating cell nuclear antigen, caspase-3, and oestrogen receptor-b. Results: Ovariectomised rats showed signs of degeneration in all zones of adrenal cortex. On the other hand, treatment with genistein showed restoration of the adrenal cortex with less proliferative effect than oestradiol. Conclusions: So, genistein can be used as effective therapy to decrease the symptoms of menopause without fear of cancer development.

Abstract

Background: Genistein, a naturally occurring soy isoflavone, attracts interest as an effective and safe alternative to hormone replacement therapy for menopausal problems. The aim of the current study was to compare between the effect of genistein and oestradiol on the adrenal cortex of the ovariectomised adult female albino rats. Materials and methods: Twenty rats were used in the current study and divided into four groups, 5 rats in each group; group 1 (control non-ovariectomised), group 2 (ovariectomised), group 3 (ovariectomised + genistein) and group 4 (ovariectomised + oestradiol). The rats were sacrificed after 4 weeks. Both adrenal glands were removed for light microscope using haematoxylin and eosin stain, ultrastructural study and immunohistochemical examination using proliferating cell nuclear antigen, caspase-3, and oestrogen receptor-b. Results: Ovariectomised rats showed signs of degeneration in all zones of adrenal cortex. On the other hand, treatment with genistein showed restoration of the adrenal cortex with less proliferative effect than oestradiol. Conclusions: So, genistein can be used as effective therapy to decrease the symptoms of menopause without fear of cancer development.

Get Citation

Keywords

adrenal, menopause, genistein, oestradiol, rat

About this article
Title

Effect of genistein and oestradiol on the adrenal cortex of the ovariectomised adult female albino rats

Journal

Folia Morphologica

Issue

Vol 80, No 2 (2021)

Article type

Original article

Pages

380-391

Published online

2020-05-25

Page views

1151

Article views/downloads

1291

DOI

10.5603/FM.a2020.0056

Pubmed

32459363

Bibliographic record

Folia Morphol 2021;80(2):380-391.

Keywords

adrenal
menopause
genistein
oestradiol
rat

Authors

H. D. Yassa
N. M. Safwat
R. M. Ahmed
M. Z. Fathy
H. L. Metry

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