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ORIGINAL ARTICLES
Published online: 2019-12-30
Submitted: 2019-11-09
Accepted: 2019-12-16
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Effect of high fructose diet induced metabolic syndrome on the pituitary-gonadal axis from adolescence through adulthood in male albino rats and the possible protective role of ginger extract. A biochemical, histological and immunohistochemical study

Abeer El-said El-Mehi, Manar Ali Faried
DOI: 10.5603/FM.a2019.0139
·
Pubmed: 31886878

open access

Ahead of Print
ORIGINAL ARTICLES
Published online: 2019-12-30
Submitted: 2019-11-09
Accepted: 2019-12-16

Abstract

Background: This work was designed to clarify, for the first time up to our knowledge, the effects of high fructose diet (HFrD) induced metabolic syndrome (MetS) on the pituitary – gonadal axis of adolescent male rats continuing through adulthood period and to evaluate the possible role of ginger extract in ameliorating these effects.

Materials and methods: Forty 4-week-old male albino rats, treated for 8 weeks, were randomly divided into 4 equal groups; control(fed standard diet), ginger extract treated (500 mg/kg once daily orally by gavage), HFrD induced MetS (fed a diet containing 60% fructose), HFrD and ginger groups. The assessment methods included biochemical, histological and immunohistochemical studies.

Results: HFrD fed rats exhibited a picture similar to MetS in the form of increased body weight and serum levels of glucose and insulin with an elevated HOMA-IR reflecting insulin resistance as well as dyslipidemia. Lipid peroxidation (increased MDA) and oxidative stress (decreased SOD) were implicated in this syndrome. This group exhibited a significant decrease of testicular weight and the levels of reproductive hormones (LH, FSH & testosterone). The pituitary gonadotrophs showed electron dense nuclei, large cytoplasmic vacuoles, destructed organelles in addition to decreased number of secretory granules. Furthermore, testicular specimens presented marked alterations. There were disorganized shrunken tubules with irregular basement membranes, reduced germinal epithelial thickness, vacuolations and degenerated mitochondria in the spermatogenic cells. Beclin1 and PCNA immunoexpressions were significantly downregulated with HFrD induced MetS. Ginger extract supplementation proved to be a potent protective agent against these harmful effects through its antioxidant, hypoglycemic, insulinotropic, androgenic and hypolipidemic effects in addition to its ability to induce testicular autophagy.

Conclusions: Consumption of HFrD induced MetS and proved to have harmful effects on the pituitary – testicular axis. Moreover, this work provided a new insight into the possible use of ginger extract to alleviate the main features of MetS and protect the pituitary-gonadal axis from the damaging effects of HFrD induced MetS.

Abstract

Background: This work was designed to clarify, for the first time up to our knowledge, the effects of high fructose diet (HFrD) induced metabolic syndrome (MetS) on the pituitary – gonadal axis of adolescent male rats continuing through adulthood period and to evaluate the possible role of ginger extract in ameliorating these effects.

Materials and methods: Forty 4-week-old male albino rats, treated for 8 weeks, were randomly divided into 4 equal groups; control(fed standard diet), ginger extract treated (500 mg/kg once daily orally by gavage), HFrD induced MetS (fed a diet containing 60% fructose), HFrD and ginger groups. The assessment methods included biochemical, histological and immunohistochemical studies.

Results: HFrD fed rats exhibited a picture similar to MetS in the form of increased body weight and serum levels of glucose and insulin with an elevated HOMA-IR reflecting insulin resistance as well as dyslipidemia. Lipid peroxidation (increased MDA) and oxidative stress (decreased SOD) were implicated in this syndrome. This group exhibited a significant decrease of testicular weight and the levels of reproductive hormones (LH, FSH & testosterone). The pituitary gonadotrophs showed electron dense nuclei, large cytoplasmic vacuoles, destructed organelles in addition to decreased number of secretory granules. Furthermore, testicular specimens presented marked alterations. There were disorganized shrunken tubules with irregular basement membranes, reduced germinal epithelial thickness, vacuolations and degenerated mitochondria in the spermatogenic cells. Beclin1 and PCNA immunoexpressions were significantly downregulated with HFrD induced MetS. Ginger extract supplementation proved to be a potent protective agent against these harmful effects through its antioxidant, hypoglycemic, insulinotropic, androgenic and hypolipidemic effects in addition to its ability to induce testicular autophagy.

Conclusions: Consumption of HFrD induced MetS and proved to have harmful effects on the pituitary – testicular axis. Moreover, this work provided a new insight into the possible use of ginger extract to alleviate the main features of MetS and protect the pituitary-gonadal axis from the damaging effects of HFrD induced MetS.

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Keywords

high fructose, metabolic syndrome, ginger, pituitary, testis, oxidative stress, histopathology, immunohistochemistry

About this article
Title

Effect of high fructose diet induced metabolic syndrome on the pituitary-gonadal axis from adolescence through adulthood in male albino rats and the possible protective role of ginger extract. A biochemical, histological and immunohistochemical study

Journal

Folia Morphologica

Issue

Ahead of Print

Published online

2019-12-30

DOI

10.5603/FM.a2019.0139

Pubmed

31886878

Keywords

high fructose
metabolic syndrome
ginger
pituitary
testis
oxidative stress
histopathology
immunohistochemistry

Authors

Abeer El-said El-Mehi
Manar Ali Faried

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