open access

Ahead of Print
ORIGINAL ARTICLES
Published online: 2020-01-07
Submitted: 2019-11-06
Accepted: 2019-12-14
Get Citation

Calvarial bone defects in ovariectomized rats treated with mesenchymal stem cells and demineralized freeze-dried bone allografts

E. T. Kadiroğlu, M. E. Akbalık, E. Karaöz, B. E. Kanay, A. Dağ, M. A. Ketani, E. G. Eroğlu, E. Uysal, M. C. Tuncer
DOI: 10.5603/FM.a2020.0001
·
Pubmed: 31930468

open access

Ahead of Print
ORIGINAL ARTICLES
Published online: 2020-01-07
Submitted: 2019-11-06
Accepted: 2019-12-14

Abstract

The aim of the study was to investigate the ability of a combination of bone marrow mesenchymal stem cells (BM-MSCs) with and without demineralized freeze-dried bone allografts (DFDBAs) to induce bone regeneration in calvarial defects in ovariectomized rats. Critical size defects were filled with a combination of demineralized freeze-dried bone allografts and bone marrow mesenchymal stem cells (BM-MSCs) or BM-MSCs alone. Eight weeks after calvarial surgery, the rats were sacrificed. The samples were analyzed histologically and immunohistochemically. No difference was observed in vascularization between groups C1 (animals with cranial defect only, control group) and O1 (animals with cranial defect only, ovariectomy group). Intramembranous ossification was observed at a limited level in groups C2 (animals with cranial defect with MSCs, control group) and O2 (animals with cranial defect with MSCs, ovariectomy group) compared to C1 and O1. In group C3 (animals with demineralized freeze-dried bone allografts with MSCs, control group), the fibrous structures of the matrix became compact as a result of a bone graft having been placed in the cavity, but in group O3 (animals with demineralized freeze-dried bone allografts with MSCs, ovariectomy group), the fibrous tissue was poorly distributed between the bone grafts for the most parts. We conclude that the insertion of BM-MSCs enhances bone healing; however, the DFDBA/BM-MSC combination has little effect on overcoming impaired bone formation in ovariectomized rats.

Abstract

The aim of the study was to investigate the ability of a combination of bone marrow mesenchymal stem cells (BM-MSCs) with and without demineralized freeze-dried bone allografts (DFDBAs) to induce bone regeneration in calvarial defects in ovariectomized rats. Critical size defects were filled with a combination of demineralized freeze-dried bone allografts and bone marrow mesenchymal stem cells (BM-MSCs) or BM-MSCs alone. Eight weeks after calvarial surgery, the rats were sacrificed. The samples were analyzed histologically and immunohistochemically. No difference was observed in vascularization between groups C1 (animals with cranial defect only, control group) and O1 (animals with cranial defect only, ovariectomy group). Intramembranous ossification was observed at a limited level in groups C2 (animals with cranial defect with MSCs, control group) and O2 (animals with cranial defect with MSCs, ovariectomy group) compared to C1 and O1. In group C3 (animals with demineralized freeze-dried bone allografts with MSCs, control group), the fibrous structures of the matrix became compact as a result of a bone graft having been placed in the cavity, but in group O3 (animals with demineralized freeze-dried bone allografts with MSCs, ovariectomy group), the fibrous tissue was poorly distributed between the bone grafts for the most parts. We conclude that the insertion of BM-MSCs enhances bone healing; however, the DFDBA/BM-MSC combination has little effect on overcoming impaired bone formation in ovariectomized rats.

Get Citation

Keywords

bone healing, bone marrow mesenchymal stem cells (BM-MSCs), demineralized freeze-dried bone allografts (DFDBAs), ovariectomy, calvarial defect

About this article
Title

Calvarial bone defects in ovariectomized rats treated with mesenchymal stem cells and demineralized freeze-dried bone allografts

Journal

Folia Morphologica

Issue

Ahead of Print

Published online

2020-01-07

DOI

10.5603/FM.a2020.0001

Pubmed

31930468

Keywords

bone healing
bone marrow mesenchymal stem cells (BM-MSCs)
demineralized freeze-dried bone allografts (DFDBAs)
ovariectomy
calvarial defect

Authors

E. T. Kadiroğlu
M. E. Akbalık
E. Karaöz
B. E. Kanay
A. Dağ
M. A. Ketani
E. G. Eroğlu
E. Uysal
M. C. Tuncer

Important: This website uses cookies. More >>

The cookies allow us to identify your computer and find out details about your last visit. They remembering whether you've visited the site before, so that you remain logged in - or to help us work out how many new website visitors we get each month. Most internet browsers accept cookies automatically, but you can change the settings of your browser to erase cookies or prevent automatic acceptance if you prefer.

By  "Via Medica sp. z o.o." sp.k., Świętokrzyska 73, 80–180 Gdańsk, Poland

tel.:+48 58 320 94 94, faks:+48 58 320 94 60, e-mail:  viamedica@viamedica.pl