Vol 61, No 4 (2002)
Original article
Submitted: 2012-02-06
Published online: 2002-08-29
Distribution of nitric oxide synthase and neuropeptide Y neurones during the development of the hippocampal formation in the rat
Joanna M. Moryś, Przemysław Kowiański, Janusz Moryś
Folia Morphol 2002;61(4):221-232.
Vol 61, No 4 (2002)
ORIGINAL ARTICLES
Submitted: 2012-02-06
Published online: 2002-08-29
Abstract
Nitric oxide (NO) is a short-lived radical, which modulates synaptic plasticity, neuronal
oscillations and cerebral blood flow. NOS-containing neurones can be detected
anatomically by nicotinamide adenine dinucleotide phosphate-diaphorase
(NADPH-d) histochemistry or by NOS immunohistochemistry. Neuropeptide Y (NPY)
is the most abundant peptide in the brain. NPY is connected with several vital
functions, such as a feeding behaviour, sexual maturation, regulation of circadian
rhythms, body temperature, blood pressure and neuroendocrine secretions. Neuropeptide
Y also modulates anxiety-related disorders, limbic epileptic seizures as
well as learning and memory processes. The study was performed on 45 Wistar
rats of various ages (P0, P4, P7, P10, P14, P21, P30, P60, and P120; P - postnatal
day). The free-floating sections were stained with standard immunohistochemistry
methods. Thereafter the histological sections were studied using
the confocal laser microscope equipped. For 3D reconstruction the image analysis
program LaserSharp 2000v. 2.0 (Bio-Rad, UK) was used. We found that in
the newborn rat both NOS- and NPY-immunoreactivity was weak. It had been
increasing gradually until the 7th day of postnatal life, after that until P14 it
was maintained on the similar level, and then the number of immunolabelled
cells deceased. The developmental changes concerned cell morphology as well
- until the 10th day of life the immunoreactive cells were immature, with round
or oval bodies and had only a few fibres. From P14 the cells’ morphology became
similar to that in adult.
Abstract
Nitric oxide (NO) is a short-lived radical, which modulates synaptic plasticity, neuronal
oscillations and cerebral blood flow. NOS-containing neurones can be detected
anatomically by nicotinamide adenine dinucleotide phosphate-diaphorase
(NADPH-d) histochemistry or by NOS immunohistochemistry. Neuropeptide Y (NPY)
is the most abundant peptide in the brain. NPY is connected with several vital
functions, such as a feeding behaviour, sexual maturation, regulation of circadian
rhythms, body temperature, blood pressure and neuroendocrine secretions. Neuropeptide
Y also modulates anxiety-related disorders, limbic epileptic seizures as
well as learning and memory processes. The study was performed on 45 Wistar
rats of various ages (P0, P4, P7, P10, P14, P21, P30, P60, and P120; P - postnatal
day). The free-floating sections were stained with standard immunohistochemistry
methods. Thereafter the histological sections were studied using
the confocal laser microscope equipped. For 3D reconstruction the image analysis
program LaserSharp 2000v. 2.0 (Bio-Rad, UK) was used. We found that in
the newborn rat both NOS- and NPY-immunoreactivity was weak. It had been
increasing gradually until the 7th day of postnatal life, after that until P14 it
was maintained on the similar level, and then the number of immunolabelled
cells deceased. The developmental changes concerned cell morphology as well
- until the 10th day of life the immunoreactive cells were immature, with round
or oval bodies and had only a few fibres. From P14 the cells’ morphology became
similar to that in adult.
Keywords
nitric oxide; neuropeptide Y; hippocampus; development; rat
Title
Distribution of nitric oxide synthase and neuropeptide Y neurones during the development of the hippocampal formation in the rat
Journal
Folia Morphologica
Issue
Vol 61, No 4 (2002)
Article type
Original article
Pages
221-232
Published online
2002-08-29
Page views
436
Article views/downloads
1187
Bibliographic record
Folia Morphol 2002;61(4):221-232.
Keywords
nitric oxide
neuropeptide Y
hippocampus
development
rat
Authors
Joanna M. Moryś
Przemysław Kowiański
Janusz Moryś