open access

Vol 63, No 1 (2004)
Short Communications
Published online: 2003-12-03
Submitted: 2012-02-06
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The activity of human telomerase in the cells of acute leukaemias

Janusz Kocki, Joanna Kolano, Maria Cioch, Anna Dmoszyńska, Jacek Wojcierowski
Folia Morphol 2004;63(1):127-128.

open access

Vol 63, No 1 (2004)
Short Communications
Published online: 2003-12-03
Submitted: 2012-02-06

Abstract

Telomeres are the end fragments of chromosomes formed by a number of non-coding double-stranded TTAGGG repeats in vertebrates. During cell division the number of repeats decreases, leading to cell senescence or apoptosis. In immortal cells, including cancer cells, the telomere length is stable and maintained by, among other factors, telomerase. The aim of the study is to compare telomerase activity in normal lymphocytes and in leukaemic cells. Samples of acute leukaemia cells, HL 60 cell line and the lymphocytes of healthy volunteers were examined. Telomerase analysis was performed using TeloTAGGG Telomerase PCR ELISAplus (Roche). The relative telomerase activities (RTA) in leukaemic and normal cells were analysed. A high level of RTA was observed in leukaemic cells.

Abstract

Telomeres are the end fragments of chromosomes formed by a number of non-coding double-stranded TTAGGG repeats in vertebrates. During cell division the number of repeats decreases, leading to cell senescence or apoptosis. In immortal cells, including cancer cells, the telomere length is stable and maintained by, among other factors, telomerase. The aim of the study is to compare telomerase activity in normal lymphocytes and in leukaemic cells. Samples of acute leukaemia cells, HL 60 cell line and the lymphocytes of healthy volunteers were examined. Telomerase analysis was performed using TeloTAGGG Telomerase PCR ELISAplus (Roche). The relative telomerase activities (RTA) in leukaemic and normal cells were analysed. A high level of RTA was observed in leukaemic cells.
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Keywords

telomeres; telomerase; human acute leukaemia cells

About this article
Title

The activity of human telomerase in the cells of acute leukaemias

Journal

Folia Morphologica

Issue

Vol 63, No 1 (2004)

Pages

127-128

Published online

2003-12-03

Bibliographic record

Folia Morphol 2004;63(1):127-128.

Keywords

telomeres
telomerase
human acute leukaemia cells

Authors

Janusz Kocki
Joanna Kolano
Maria Cioch
Anna Dmoszyńska
Jacek Wojcierowski

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