open access

Vol 63, No 1 (2004)
Short Communications
Published online: 2003-12-03
Submitted: 2012-02-06
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Bone marrow morphology during haematopoietic stem cell mobilization with G-CSF in mice

Kamila Barcew, Ewa Kacińska, Mariola Marchlewicz, Barbara Wiszniewska, Bogusław Machaliński
Folia Morphol 2004;63(1):87-89.

open access

Vol 63, No 1 (2004)
Short Communications
Published online: 2003-12-03
Submitted: 2012-02-06

Abstract

The aim of the work was to examine the morphology of the bone marrow of mice during stimulation with G-CSF. Experimental Balb C mice were daily injected subcutaneously with 250 μg/kg b.w. G-CSF (Neupogen). After 2, 4 and 6 days of the experiment femurs were obtained for morphological study. On day 2 of the mobilization the amount of haematopoietic cells in the bone marrow increased and dilatation of the sinusoids was observed. Only single leukocytes were observed in the lumen of the vessels. There were numerous leukocytes in the lumen of the sinusoids on day 4 of the mobilization. The morphology of the bone marrow on day 6 was similar to that of the control. Mobilization of mice with G-CSF resulted in migration of haematopoietic cells from the bone marrow and the process is most pronounced on day 4.

Abstract

The aim of the work was to examine the morphology of the bone marrow of mice during stimulation with G-CSF. Experimental Balb C mice were daily injected subcutaneously with 250 μg/kg b.w. G-CSF (Neupogen). After 2, 4 and 6 days of the experiment femurs were obtained for morphological study. On day 2 of the mobilization the amount of haematopoietic cells in the bone marrow increased and dilatation of the sinusoids was observed. Only single leukocytes were observed in the lumen of the vessels. There were numerous leukocytes in the lumen of the sinusoids on day 4 of the mobilization. The morphology of the bone marrow on day 6 was similar to that of the control. Mobilization of mice with G-CSF resulted in migration of haematopoietic cells from the bone marrow and the process is most pronounced on day 4.
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Keywords

bone marrow morphology; sinusoids; haematopoietic cells; G-CSF; mobilization

About this article
Title

Bone marrow morphology during haematopoietic stem cell mobilization with G-CSF in mice

Journal

Folia Morphologica

Issue

Vol 63, No 1 (2004)

Pages

87-89

Published online

2003-12-03

Bibliographic record

Folia Morphol 2004;63(1):87-89.

Keywords

bone marrow morphology
sinusoids
haematopoietic cells
G-CSF
mobilization

Authors

Kamila Barcew
Ewa Kacińska
Mariola Marchlewicz
Barbara Wiszniewska
Bogusław Machaliński

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