open access

Vol 63, No 1 (2004)
SHORT COMMUNICATIONS
Published online: 2003-12-03
Submitted: 2012-02-06
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The effect of extracellular substance components on the proliferation and expression of hormones in cultured cells of medullary thyroid carcinomas

Maciej Zabel, Elżbieta Gębarowska, Joanna Surdyk-Zasada, Izabela Lesisz, Aldona Kasprzak, Jolanta Seidel
Folia Morphol 2004;63(1):63-66.

open access

Vol 63, No 1 (2004)
SHORT COMMUNICATIONS
Published online: 2003-12-03
Submitted: 2012-02-06

Abstract

The effects were examined of selected extracellular medium (ECM) components on the proliferation of medullary thyroid carcinoma cells and on the production of calcitonin and CGRP. Human TT cells and rat rMTC cells were cultured for 24, 48 and 72 hours on glass coated with type I collagen, fibronectin or poly-D-lysine. More pronounced proliferation was demonstrated by TT cells grown on poly-Dlysine or collagen in comparison with the control and less pronounced proliferation was typical of cells grown on fibronectin. On the other hand, rMTC cells were more markedly manifest on any ECM substrates than that on glass. Alterations in the proliferation were paralleled by changes in the expression of CT and CGRP.

Abstract

The effects were examined of selected extracellular medium (ECM) components on the proliferation of medullary thyroid carcinoma cells and on the production of calcitonin and CGRP. Human TT cells and rat rMTC cells were cultured for 24, 48 and 72 hours on glass coated with type I collagen, fibronectin or poly-D-lysine. More pronounced proliferation was demonstrated by TT cells grown on poly-Dlysine or collagen in comparison with the control and less pronounced proliferation was typical of cells grown on fibronectin. On the other hand, rMTC cells were more markedly manifest on any ECM substrates than that on glass. Alterations in the proliferation were paralleled by changes in the expression of CT and CGRP.
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Keywords

medullary thyroid carcinoma; cell culture; extracellular matrix; calcitonin; CGRP

About this article
Title

The effect of extracellular substance components on the proliferation and expression of hormones in cultured cells of medullary thyroid carcinomas

Journal

Folia Morphologica

Issue

Vol 63, No 1 (2004)

Pages

63-66

Published online

2003-12-03

Bibliographic record

Folia Morphol 2004;63(1):63-66.

Keywords

medullary thyroid carcinoma
cell culture
extracellular matrix
calcitonin
CGRP

Authors

Maciej Zabel
Elżbieta Gębarowska
Joanna Surdyk-Zasada
Izabela Lesisz
Aldona Kasprzak
Jolanta Seidel

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