Vol 63, No 1 (2004)
Short communication
Published online: 2003-12-03
The effect of extracellular substance components on the proliferation and expression of hormones in cultured cells of medullary thyroid carcinomas
Folia Morphol 2004;63(1):63-66.
Abstract
The effects were examined of selected extracellular medium (ECM) components
on the proliferation of medullary thyroid carcinoma cells and on the production of
calcitonin and CGRP. Human TT cells and rat rMTC cells were cultured for 24, 48
and 72 hours on glass coated with type I collagen, fibronectin or poly-D-lysine.
More pronounced proliferation was demonstrated by TT cells grown on poly-Dlysine
or collagen in comparison with the control and less pronounced proliferation
was typical of cells grown on fibronectin. On the other hand, rMTC cells were
more markedly manifest on any ECM substrates than that on glass. Alterations in
the proliferation were paralleled by changes in the expression of CT and CGRP.
Keywords: medullary thyroid carcinomacell cultureextracellular matrixcalcitoninCGRP