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Proliferation and apoptosis in the guinea pig adrenal cortex during postnatal development
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Abstract
The study was carried out with adrenal glands from guinea pigs aged 1, 3, 9, 14, 21, 35 and 90 days. Paraffin slices were stained with Mayer haematoxylin and eosin and with acid fuchsin. For the immunohistochemical reaction the Anti -PCNA Clone PC10 (Dako) was used. Apoptosis evaluation was performed with use of the TUNEL method (Roche).
The results support the theory of involution of the FZ cells realised mainly by way of apoptosis. Analysis of the distribution and number of PCNA-positive cells in the adrenal cortex on subsequent days of PD may also back up the migration theory of renewal of the adrenal cortex cells.
Abstract
The study was carried out with adrenal glands from guinea pigs aged 1, 3, 9, 14, 21, 35 and 90 days. Paraffin slices were stained with Mayer haematoxylin and eosin and with acid fuchsin. For the immunohistochemical reaction the Anti -PCNA Clone PC10 (Dako) was used. Apoptosis evaluation was performed with use of the TUNEL method (Roche).
The results support the theory of involution of the FZ cells realised mainly by way of apoptosis. Analysis of the distribution and number of PCNA-positive cells in the adrenal cortex on subsequent days of PD may also back up the migration theory of renewal of the adrenal cortex cells.
Keywords
adrenal gland; foetal zone; PCNA
Title
Proliferation and apoptosis in the guinea pig adrenal cortex during postnatal development
Journal
Issue
Article type
Short communication
Pages
333-335
Published online
2004-06-04
Page views
461
Article views/downloads
1748
Bibliographic record
Folia Morphol 2004;63(3):333-335.
Keywords
adrenal gland
foetal zone
PCNA
Authors
D Kaczmarczyk
BL Kmieć
M Daczewska
KB Kmieć