open access

Vol 64, No 4 (2005)
ORIGINAL ARTICLES
Published online: 2005-09-20
Submitted: 2012-02-06
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Mimicking of glutathione peroxidase deficiency by exposition of JAR cells to increased level of synthetic hydroperoxide

Hallmann A, Milczarek R, Szkatuła M, Woźniak M, Spodnik JH, Klimek J
Folia Morphol 2005;64(4):304-308.

open access

Vol 64, No 4 (2005)
ORIGINAL ARTICLES
Published online: 2005-09-20
Submitted: 2012-02-06

Abstract

A short chain synthetic analogue of lipid hydroperoxides was used to overload glutathione peroxidase (GPx) in human choriocarcinoma cell line JAR cells. Cells exposed to 100 µM tBuOOH displayed a 40% reduction in ATP level and significantly increased in membrane permeability, visualised by the lactate dehydrogenase (LDH) release into the extracellular medium. The intracellular level of oxygen free radicals measured as an oxidation of the dichlorodihydro-fluorescein diacetate (H2DCF-DA) significantly increased after 2 hours of cell exposition to 100 µM tBuOOH. Concomitantly MDA, 4-HNE level increased to 2 nmol/mg of cell protein after 2 hours. Mitochondria stained with MitoTracker Red CMXRos displayed a filamentous appearance in control cells but changed into granular less energised organelles after exposition to tBuOOH. Collectively, the above results indicate the importance of the contribution of oxidative stress in the development of pre-eclampsia.

Abstract

A short chain synthetic analogue of lipid hydroperoxides was used to overload glutathione peroxidase (GPx) in human choriocarcinoma cell line JAR cells. Cells exposed to 100 µM tBuOOH displayed a 40% reduction in ATP level and significantly increased in membrane permeability, visualised by the lactate dehydrogenase (LDH) release into the extracellular medium. The intracellular level of oxygen free radicals measured as an oxidation of the dichlorodihydro-fluorescein diacetate (H2DCF-DA) significantly increased after 2 hours of cell exposition to 100 µM tBuOOH. Concomitantly MDA, 4-HNE level increased to 2 nmol/mg of cell protein after 2 hours. Mitochondria stained with MitoTracker Red CMXRos displayed a filamentous appearance in control cells but changed into granular less energised organelles after exposition to tBuOOH. Collectively, the above results indicate the importance of the contribution of oxidative stress in the development of pre-eclampsia.
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Keywords

mitochondria; choriocarcinoma; oxidative stress; pre-eclampsia

About this article
Title

Mimicking of glutathione peroxidase deficiency by exposition of JAR cells to increased level of synthetic hydroperoxide

Journal

Folia Morphologica

Issue

Vol 64, No 4 (2005)

Pages

304-308

Published online

2005-09-20

Bibliographic record

Folia Morphol 2005;64(4):304-308.

Keywords

mitochondria
choriocarcinoma
oxidative stress
pre-eclampsia

Authors

Hallmann A
Milczarek R
Szkatuła M
Woźniak M
Spodnik JH
Klimek J

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