Mimicking of glutathione peroxidase deficiency by exposition of JAR cells to increased level of synthetic hydroperoxide

Hallmann A; Milczarek R; Szkatuła M; Woźniak M; Spodnik JH; Klimek J

Vol 64, No 4 (2005)

Original article

Submitted: 2012-02-06

Published online: 2005-09-20

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Mimicking of glutathione peroxidase deficiency by exposition of JAR cells to increased level of synthetic hydroperoxide

Hallmann A, Milczarek R, Szkatuła M, Woźniak M, Spodnik JH, Klimek J

Folia Morphol 2005;64(4):304-308.

Vol 64, No 4 (2005)

ORIGINAL ARTICLES

Submitted: 2012-02-06

Published online: 2005-09-20

Abstract

A short chain synthetic analogue of lipid hydroperoxides was used to overload glutathione peroxidase (GPx) in human choriocarcinoma cell line JAR cells. Cells exposed to 100 µM tBuOOH displayed a 40% reduction in ATP level and significantly increased in membrane permeability, visualised by the lactate dehydrogenase (LDH) release into the extracellular medium. The intracellular level of oxygen free radicals measured as an oxidation of the dichlorodihydro-fluorescein diacetate (H₂DCF-DA) significantly increased after 2 hours of cell exposition to 100 µM tBuOOH. Concomitantly MDA, 4-HNE level increased to 2 nmol/mg of cell protein after 2 hours. Mitochondria stained with MitoTracker Red CMXRos displayed a filamentous appearance in control cells but changed into granular less energised organelles after exposition to tBuOOH. Collectively, the above results indicate the importance of the contribution of oxidative stress in the development of pre-eclampsia.