open access

Vol 64, No 4 (2005)
ORIGINAL ARTICLES
Published online: 2005-09-20
Submitted: 2012-02-06
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An electron microscopic study of the phosphatases in the ciliate Balantidium coli

Skotarczak B, Kołodziejczyk L
Folia Morphol 2005;64(4):282-286.

open access

Vol 64, No 4 (2005)
ORIGINAL ARTICLES
Published online: 2005-09-20
Submitted: 2012-02-06

Abstract

The localisation and activity of D glucose-6-phosphatase (G-6-Pase) and alkaline phosphatase (AlP) in the trophozoites of Balantidium coli isolated from pig intestine content were investigated using ultrastructural and cytochemical methods. The activity of G-6-Pase was demonstrated on the membranes of the endoplasmic reticulum, particularly in the cortical part of the trophozoites. In addition, the product of the reaction to G-6-Pase was concentrated in the vesicular structures, which were distributed along the reticular membranes. These structures were described as vesicles similar to glycosomes, containing enzymes of glycogenolysis. It is very likely that hydrolases in B. coli are formed on the rough reticular membranes without the involvement of cisterns of the Golgi complex. The ultrastructural deposits of the reaction to G-6-Pase and AlP in the trophozoites of B. coli described here indicate that some membranes of the rough endoplasmic reticulum and small vacuoles with a strong reaction to these enzymes can play a similar role to the Golgi complex.

Abstract

The localisation and activity of D glucose-6-phosphatase (G-6-Pase) and alkaline phosphatase (AlP) in the trophozoites of Balantidium coli isolated from pig intestine content were investigated using ultrastructural and cytochemical methods. The activity of G-6-Pase was demonstrated on the membranes of the endoplasmic reticulum, particularly in the cortical part of the trophozoites. In addition, the product of the reaction to G-6-Pase was concentrated in the vesicular structures, which were distributed along the reticular membranes. These structures were described as vesicles similar to glycosomes, containing enzymes of glycogenolysis. It is very likely that hydrolases in B. coli are formed on the rough reticular membranes without the involvement of cisterns of the Golgi complex. The ultrastructural deposits of the reaction to G-6-Pase and AlP in the trophozoites of B. coli described here indicate that some membranes of the rough endoplasmic reticulum and small vacuoles with a strong reaction to these enzymes can play a similar role to the Golgi complex.
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Keywords

Ciliata; ultrastructure; phosphatases; cytochemistry

About this article
Title

An electron microscopic study of the phosphatases in the ciliate Balantidium coli

Journal

Folia Morphologica

Issue

Vol 64, No 4 (2005)

Pages

282-286

Published online

2005-09-20

Bibliographic record

Folia Morphol 2005;64(4):282-286.

Keywords

Ciliata
ultrastructure
phosphatases
cytochemistry

Authors

Skotarczak B
Kołodziejczyk L

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