open access

Vol 64, No 4 (2005)
ORIGINAL ARTICLES
Published online: 2005-09-20
Submitted: 2012-02-06
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In vivo gene transfer to the brain cortex using a single injection of HSV-1 vector into the medial septum

Tabbaa S, Corso TD, Jenkins L, Tran RK, Bloom DC, Stachowiak MK
Folia Morphol 2005;64(4):273-281.

open access

Vol 64, No 4 (2005)
ORIGINAL ARTICLES
Published online: 2005-09-20
Submitted: 2012-02-06

Abstract

This study shows that an ICP4-replication-deficient herpes simplex virus containing the Moloney murine leukaemia virus LTR fused with the coding sequence for the beta-galactosidase gene can be used as a very effective vector for delivering the beta-galactosidase reporter gene into the rat brain septum. F344 rats received bilateral stereotaxic injections into the nucleus of the diagonal band and into the medial septum. The X-gal stain was used to detect the activity of the expressed beta-galactosidase enzyme. The delivered reporter gene was expressed successfully not only in the neuronal cells of the injected areas but also in cells that project to the injection area such as cortex cells about 6 mm away from the injection sites. Expression was visible at 1, 3 and 9 weeks following injection. We conclude that this vector can effectively deliver genes into different regions of the mature mammalian brain and also to areas distant from the injection site.

Abstract

This study shows that an ICP4-replication-deficient herpes simplex virus containing the Moloney murine leukaemia virus LTR fused with the coding sequence for the beta-galactosidase gene can be used as a very effective vector for delivering the beta-galactosidase reporter gene into the rat brain septum. F344 rats received bilateral stereotaxic injections into the nucleus of the diagonal band and into the medial septum. The X-gal stain was used to detect the activity of the expressed beta-galactosidase enzyme. The delivered reporter gene was expressed successfully not only in the neuronal cells of the injected areas but also in cells that project to the injection area such as cortex cells about 6 mm away from the injection sites. Expression was visible at 1, 3 and 9 weeks following injection. We conclude that this vector can effectively deliver genes into different regions of the mature mammalian brain and also to areas distant from the injection site.
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Keywords

herpes simplex virus; gene therapy; beta-galactosidase; Alzheimer’s disease

About this article
Title

In vivo gene transfer to the brain cortex using a single injection of HSV-1 vector into the medial septum

Journal

Folia Morphologica

Issue

Vol 64, No 4 (2005)

Pages

273-281

Published online

2005-09-20

Bibliographic record

Folia Morphol 2005;64(4):273-281.

Keywords

herpes simplex virus
gene therapy
beta-galactosidase
Alzheimer’s disease

Authors

Tabbaa S
Corso TD
Jenkins L
Tran RK
Bloom DC
Stachowiak MK

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