open access

Vol 60, No 2 (2022)
Original paper
Submitted: 2022-03-19
Accepted: 2022-05-05
Published online: 2022-05-16
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Identification of candidate genes simultaneously shared by adipogenesis and osteoblastogenesis from human mesenchymal stem cells

Xia Yi1, Ping Wu1, Yunyan Fan1, Ying Gong1, Jianyun Liu1, Jianjun Xiong1, Xiaoyuan Xu1
·
Pubmed: 35575247
·
Folia Histochem Cytobiol 2022;60(2):179-190.
Affiliations
  1. Jiangxi Provincial Key Laboratory of Systems Biomedicine, Jiujiang University, Jiujiang, China

open access

Vol 60, No 2 (2022)
ORIGINAL PAPERS
Submitted: 2022-03-19
Accepted: 2022-05-05
Published online: 2022-05-16

Abstract

Introduction. In osteoporosis field, it had been clinically well established a given relationship between bone formation and lipid accumulation. Although numerous molecules had been well documented for adipogenesis and osteoblastogenesis (adipo-osteoblastogenesis), the reciprocal transcriptional regulation still remains to be explored.
Material and methods. Here, we tried to identify the common candidate genes of adipocyte/osteoblastocyte differentiation at 3, 5, and 7 days using human mesenchymal stem cells (hMSCs) via RNA-Seq technique. By using RNA interference (RNAi), we further confirmed the function of candidate genes during adipo-osteoblastogenesis through Oil Red/Alizarin Red/alkaline phosphatase (ALPL) staining and qRT-PCR (quantitative real-time PCR).
Results. The identified 275 significantly differentially expressed genes (DEGs), especially with the down-regulated genes most prevalent and PI3K-AKT signaling pathway mostly enriched, were simultaneously shared by both differentiation events. Using lentiviral system, we further confirmed that ANKRD1 (ankyrin repeat domain 1) promoted adipogenesis and inhibited osteoblastogenesis via RNA interference (RNAi), and IGF1 (insulin like growth factor 1) simultaneously facilitated adipo-osteoblastogenesis on the base of gene expression of biomarkers and cellular phenotype property.
Conclusion. This study would provide the potential molecular switches to control the adipocyte/osteoblastocyte balance or hMSCs fate choices and clues to screen the study and therapy targets of metabolic bone disease osteoporosis.

Abstract

Introduction. In osteoporosis field, it had been clinically well established a given relationship between bone formation and lipid accumulation. Although numerous molecules had been well documented for adipogenesis and osteoblastogenesis (adipo-osteoblastogenesis), the reciprocal transcriptional regulation still remains to be explored.
Material and methods. Here, we tried to identify the common candidate genes of adipocyte/osteoblastocyte differentiation at 3, 5, and 7 days using human mesenchymal stem cells (hMSCs) via RNA-Seq technique. By using RNA interference (RNAi), we further confirmed the function of candidate genes during adipo-osteoblastogenesis through Oil Red/Alizarin Red/alkaline phosphatase (ALPL) staining and qRT-PCR (quantitative real-time PCR).
Results. The identified 275 significantly differentially expressed genes (DEGs), especially with the down-regulated genes most prevalent and PI3K-AKT signaling pathway mostly enriched, were simultaneously shared by both differentiation events. Using lentiviral system, we further confirmed that ANKRD1 (ankyrin repeat domain 1) promoted adipogenesis and inhibited osteoblastogenesis via RNA interference (RNAi), and IGF1 (insulin like growth factor 1) simultaneously facilitated adipo-osteoblastogenesis on the base of gene expression of biomarkers and cellular phenotype property.
Conclusion. This study would provide the potential molecular switches to control the adipocyte/osteoblastocyte balance or hMSCs fate choices and clues to screen the study and therapy targets of metabolic bone disease osteoporosis.

Get Citation

Keywords

human mesenchymal stem cells; adipogenesis; osteoblastogenesis; ANKRD1; IGF1

About this article
Title

Identification of candidate genes simultaneously shared by adipogenesis and osteoblastogenesis from human mesenchymal stem cells

Journal

Folia Histochemica et Cytobiologica

Issue

Vol 60, No 2 (2022)

Article type

Original paper

Pages

179-190

Published online

2022-05-16

Page views

4673

Article views/downloads

617

DOI

10.5603/FHC.a2022.0012

Pubmed

35575247

Bibliographic record

Folia Histochem Cytobiol 2022;60(2):179-190.

Keywords

human mesenchymal stem cells
adipogenesis
osteoblastogenesis
ANKRD1
IGF1

Authors

Xia Yi
Ping Wu
Yunyan Fan
Ying Gong
Jianyun Liu
Jianjun Xiong
Xiaoyuan Xu

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