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Vol 60, No 1 (2022)
Original paper
Submitted: 2021-08-02
Accepted: 2022-01-28
Published online: 2022-02-14
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Wnt/PCP pathway regulates the migration and neural differentiation of mesenchymal stem cells in vitro

Panpan Yao1, Qin Yu1, Lujie Zhu1, Jingxian Li1, Xueyuan Zhou1, Lili Wu1, Yongyi Cai1, Hongmei Shen1, Liping Zhou1
DOI: 10.5603/FHC.a2022.0006
·
Pubmed: 35156188
·
Folia Histochem Cytobiol 2022;60(1):44-54.
Affiliations
  1. College of Life Science, Zhejiang Chinese Medical University, Hangzhou, Zhejiang 310053, PR China

open access

Vol 60, No 1 (2022)
ORIGINAL PAPERS
Submitted: 2021-08-02
Accepted: 2022-01-28
Published online: 2022-02-14

Abstract

Introduction. Mesenchymal stem cells (MSCs) are an excellent donor graft source due to their potential for self-renewal and multidirectional differentiation. However, the potential mechanisms involved in MSC homing and neural differentiation are still unclear. The purpose of this study was to explore the effects of a chemokine, SDF-1a, and Wnt3a ligand on rat MSCs’ migration and b-mercaptoethanol (BME)-induced neural differentiation of MSCs.

Materials and methods. MSCs were isolated from rat bone marrow and cultured in vitro to passage 3. Scratch tests and transwell assays were used to estimate the effects of SDF-1a (25 ng/mL) and Wnt3a (10 ng/mL) on the migration of MSCs. The expression of Wnt/PCP pathway proteins RhoA, c-Jun, ATF2, and Wnt3a were assessed by Western blot. The 5 mM BME-induced neural differentiation of MSCs was determined by immunofluorescence to detect neuron- and astrocyte-specific markers such as nestin, GFAP, and Olig2.

Results. Wnt3a promoted the migration ability of MSCs and regulated the expression of RhoA, c-Jun, and ATF2 proteins. MSCs could differentiate into neural stem cells and astrocytes. Wnt3a enhanced BME induced neurogenesis in MSCs by increasing the protein expression of RhoA, c-Jun, and Wnt3a.

Conclusions. The present study demonstrated that the Wnt/PCP pathway promotes migration and neural differentiation of rat MSC.

Abstract

Introduction. Mesenchymal stem cells (MSCs) are an excellent donor graft source due to their potential for self-renewal and multidirectional differentiation. However, the potential mechanisms involved in MSC homing and neural differentiation are still unclear. The purpose of this study was to explore the effects of a chemokine, SDF-1a, and Wnt3a ligand on rat MSCs’ migration and b-mercaptoethanol (BME)-induced neural differentiation of MSCs.

Materials and methods. MSCs were isolated from rat bone marrow and cultured in vitro to passage 3. Scratch tests and transwell assays were used to estimate the effects of SDF-1a (25 ng/mL) and Wnt3a (10 ng/mL) on the migration of MSCs. The expression of Wnt/PCP pathway proteins RhoA, c-Jun, ATF2, and Wnt3a were assessed by Western blot. The 5 mM BME-induced neural differentiation of MSCs was determined by immunofluorescence to detect neuron- and astrocyte-specific markers such as nestin, GFAP, and Olig2.

Results. Wnt3a promoted the migration ability of MSCs and regulated the expression of RhoA, c-Jun, and ATF2 proteins. MSCs could differentiate into neural stem cells and astrocytes. Wnt3a enhanced BME induced neurogenesis in MSCs by increasing the protein expression of RhoA, c-Jun, and Wnt3a.

Conclusions. The present study demonstrated that the Wnt/PCP pathway promotes migration and neural differentiation of rat MSC.

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Keywords

mesenchymal stem cells; SDF-1a; migration; neural differentiation; Wnt3a; Wnt/PCP signaling

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About this article
Title

Wnt/PCP pathway regulates the migration and neural differentiation of mesenchymal stem cells in vitro

Journal

Folia Histochemica et Cytobiologica

Issue

Vol 60, No 1 (2022)

Article type

Original paper

Pages

44-54

Published online

2022-02-14

Page views

6012

Article views/downloads

793

DOI

10.5603/FHC.a2022.0006

Pubmed

35156188

Bibliographic record

Folia Histochem Cytobiol 2022;60(1):44-54.

Keywords

mesenchymal stem cells
SDF-1a
migration
neural differentiation
Wnt3a
Wnt/PCP signaling

Authors

Panpan Yao
Qin Yu
Lujie Zhu
Jingxian Li
Xueyuan Zhou
Lili Wu
Yongyi Cai
Hongmei Shen
Liping Zhou

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