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Genistein exerts a cell-protective effect via Nrf2/HO-1/ /PI3K signaling in Ab25-35-induced Alzheimer’s disease models in vitro
Affiliations- The First Affiliated Hospital, University of South China, Hengyang, Hunan 421001, PR China
- Second People’s Hospital of Shaoyang City, Shaoyang, Hunan 422001, PR China
- The Affiliated Nanhua Hospital, University of South China, Hengyang, Hunan 421001, PR China
open access
Abstract
Introduction. Alzheimer’s disease (AD), a very common neurodegenerative disorder, is mainly characterized by the deposition of b-amyloid protein (Ab) and extensive neuronal cell death. Currently, there are no satisfactory therapeutic approaches for AD. Although neuroprotective effects of genistein against Ab-induced toxicity have been reported, the underlying molecular mechanisms remain unclear. Furthermore, the PI3K/Akt/Nrf2 signaling pathway is associated with AD. The aim of the study was to investigate whether genistein can modulate Nrf2/HO-1/PI3K signaling to treat AD.
Materials and methods. Cell viability assay, the measurement of heme oxygenase-1 (HO-1) expression by reverse transcription-polymerase chain reaction (RT-qPCR), and western blot were performed on the SH-SY5Y cells induced by Ab25–35 in response to the treatment with genistein. Moreover, PI3K p85 phosphorylation was measured.
Results. Genistein enhanced the HO-1expression at both the mRNA and protein levels, as well as the PI3K p85 phosphorylation level. In addition, genistein increased the survival of SH-SY5Y cells treated with Ab25–35via HO-1 signaling. However, following transfection with Nrf2 small interfering RNA (siRNA) and treatment with LY294002, an inhibitor of PI3K p85, genistein could not upregulate HO-1 to exert neuroprotective effects on SH-SY5Y cells treated with Ab25–35.
Conclusions. These results suggest that genistein exerts a neuroprotective effect on SH-SY5Y cells in vitro via Nrf2/ HO-1/PI3K signaling, providing a foundation for the application of genistein in the treatment of neurodegenerative diseases related to Nrf2/HO-1/PI3K signaling.
Abstract
Introduction. Alzheimer’s disease (AD), a very common neurodegenerative disorder, is mainly characterized by the deposition of b-amyloid protein (Ab) and extensive neuronal cell death. Currently, there are no satisfactory therapeutic approaches for AD. Although neuroprotective effects of genistein against Ab-induced toxicity have been reported, the underlying molecular mechanisms remain unclear. Furthermore, the PI3K/Akt/Nrf2 signaling pathway is associated with AD. The aim of the study was to investigate whether genistein can modulate Nrf2/HO-1/PI3K signaling to treat AD.
Materials and methods. Cell viability assay, the measurement of heme oxygenase-1 (HO-1) expression by reverse transcription-polymerase chain reaction (RT-qPCR), and western blot were performed on the SH-SY5Y cells induced by Ab25–35 in response to the treatment with genistein. Moreover, PI3K p85 phosphorylation was measured.
Results. Genistein enhanced the HO-1expression at both the mRNA and protein levels, as well as the PI3K p85 phosphorylation level. In addition, genistein increased the survival of SH-SY5Y cells treated with Ab25–35via HO-1 signaling. However, following transfection with Nrf2 small interfering RNA (siRNA) and treatment with LY294002, an inhibitor of PI3K p85, genistein could not upregulate HO-1 to exert neuroprotective effects on SH-SY5Y cells treated with Ab25–35.
Conclusions. These results suggest that genistein exerts a neuroprotective effect on SH-SY5Y cells in vitro via Nrf2/ HO-1/PI3K signaling, providing a foundation for the application of genistein in the treatment of neurodegenerative diseases related to Nrf2/HO-1/PI3K signaling.
Keywords
Alzheimer’s disease; amyloid b25–35; in vitro; SH-SY5Y cells; genistein; heme oxygenase-1; Nrf2; siRNA; Nrf2/HO-1/PI3K pathway
About this articleTitle
Genistein exerts a cell-protective effect via Nrf2/HO-1/ /PI3K signaling in Ab25-35-induced Alzheimer’s disease models in vitro
Journal
Folia Histochemica et Cytobiologica
Issue
Article type
Original paper
Pages
49-56
Published online
2021-02-18
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2157
Article views/downloads
1178
DOI
Pubmed
Bibliographic record
Folia Histochem Cytobiol 2021;59(1):49-56.
Keywords
Alzheimer’s disease
amyloid b25–35
in vitro
SH-SY5Y cells
genistein
heme oxygenase-1
Nrf2
siRNA
Nrf2/HO-1/PI3K pathway
Authors
Shanqing Yi
Shuangxi Chen
Jian Xiang
Jian Tan
Kailiang Huang
Hao Zhang
Yilin Wang
Heng Wu
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