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Expression of angiogenic factor with G patch and FHA domains 1 (AGGF1) in placenta from patients with preeclampsia
Affiliations- Department of Obstetrics and Gynecology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China
open access
Abstract
Introduction. Preeclampsia (PE) is a major contributor to maternal and foetal morbidity and mortality worldwide. It manifests as high blood pressure and proteinuria in women at more than 20 weeks of gestation. Abnormal levels of anti- and pro-angiogenesis factors are known to be associated with PE. In the present study, we aimed to determine the localisation of angiogenic factor with G patch and FHA domains 1 (AGGF1) in the placenta and to compare the expression levels of AGGF1 in the third-trimester placentas of preeclamptic and normotensive pregnancies.
Materials and methods. Placental tissue samples were collected from women with PE (n = 28) and without PE (n = 28). The normotensive controls without PE were matched for gestational age at delivery with the patients with PE. The expression levels of AGGF1 in the placental tissues were evaluated using immunohistochemistry, quantitative reverse transcription polymerase chain reaction and Western blot.
Results. The immunoexpression of AGGF1 was localised in the syncytiotrophoblast tissue. Notable, the mRNA and protein expression levels of AGGF1 were decreased in preeclamptic placentas as compared with the normotensive control group (P < 0.05).
Discussion. Our results suggest that the decreased AGGF1 in preeclamptic placentas may be related to the
pathogenesis of preeclampsia.
Abstract
Introduction. Preeclampsia (PE) is a major contributor to maternal and foetal morbidity and mortality worldwide. It manifests as high blood pressure and proteinuria in women at more than 20 weeks of gestation. Abnormal levels of anti- and pro-angiogenesis factors are known to be associated with PE. In the present study, we aimed to determine the localisation of angiogenic factor with G patch and FHA domains 1 (AGGF1) in the placenta and to compare the expression levels of AGGF1 in the third-trimester placentas of preeclamptic and normotensive pregnancies.
Materials and methods. Placental tissue samples were collected from women with PE (n = 28) and without PE (n = 28). The normotensive controls without PE were matched for gestational age at delivery with the patients with PE. The expression levels of AGGF1 in the placental tissues were evaluated using immunohistochemistry, quantitative reverse transcription polymerase chain reaction and Western blot.
Results. The immunoexpression of AGGF1 was localised in the syncytiotrophoblast tissue. Notable, the mRNA and protein expression levels of AGGF1 were decreased in preeclamptic placentas as compared with the normotensive control group (P < 0.05).
Discussion. Our results suggest that the decreased AGGF1 in preeclamptic placentas may be related to the
pathogenesis of preeclampsia.
Keywords
angiogenesis; AGGF1; preeclampsia; placenta; syncytiotrophoblast; IHC, qPCR, WB
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About this articleTitle
Expression of angiogenic factor with G patch and FHA domains 1 (AGGF1) in placenta from patients with preeclampsia
Journal
Folia Histochemica et Cytobiologica
Issue
Article type
Original paper
Pages
83-89
Published online
2020-06-30
Page views
1299
Article views/downloads
953
DOI
Pubmed
Bibliographic record
Folia Histochem Cytobiol 2020;58(2):83-89.
Keywords
angiogenesis
AGGF1
preeclampsia
placenta
syncytiotrophoblast
IHC
qPCR
WB
Authors
Lan-fen An
Shu-qi Chi
Jun Zhang
Hong-bo Wang
Wei-xiang Ouyang
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