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Vol 50, No 1 (2012)
Original paper
Submitted: 2012-04-24
Accepted: 2012-04-24
Published online: 2012-04-25
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Predictive value of ERCC1 single-nucleotide polymorphism in patients receiving platinum-based chemotherapy for locally-advanced and advanced non-small cell lung cancer — a pilot study

Paweł Krawczyk, Kamila Wojas-Krawczyk, Radosław Mlak, Tomasz Kucharczyk, Beata Biernacka, Janusz Milanowski
DOI: 10.5603/FHC.2012.0011
·
Folia Histochem Cytobiol 2012;50(1):80-86.

open access

Vol 50, No 1 (2012)
ORIGINAL PAPERS
Submitted: 2012-04-24
Accepted: 2012-04-24
Published online: 2012-04-25

Abstract

Platinum-based chemotherapy is the main type of I-line treatment of advanced and non-operative NSCLC patients without EGFR gene mutation. The excision repair cross-complementation group 1 (ERCC1) is an enzyme that executes the incision of the damaged DNA strand and removes platinum-induced DNA adducts. We investigated whether ERCC1 gene polymorphism has an effect on the response to chemotherapy and survival in 43 patients with NSCLC treated with platinum-based chemotherapy. ERCC1 19007 T>C SNPs were assessed using a PCR-RFLP methods in DNA isolated from peripheral blood lymphocytes. Disease control occurred significantly (p = 0.045) more frequently in patients with CC or CT genotype compared to patients with TT genotype. Median PFS and OS for CC homozygous were 4 and 10.5 months, 4 and 12.5 months for CT heterozygous, but only 0.3 and 1.5 months for TT homozygous patients, respectively. The probability of PFS was significantly higher (HR = 0.438, 95% CI: 0.084–0.881, p = 0.03) and probability of OS was insignificantly
higher (HR = 0.503, 95% CI: 0.129–1.137, p = 0.084) in patients with CC or CT genotype than in patients with TT genotype. Uncommon TT genotype of ERCC1 19007 T>C polymorphism could predict poor response and shortening of progression free survival in NSCLC patients treated with platinum-based I-line chemotherapy. The analysis of this polymorphism may serve as a promising tool in the qualification of advanced NSCLC patients for appropriate chemotherapy.

Abstract

Platinum-based chemotherapy is the main type of I-line treatment of advanced and non-operative NSCLC patients without EGFR gene mutation. The excision repair cross-complementation group 1 (ERCC1) is an enzyme that executes the incision of the damaged DNA strand and removes platinum-induced DNA adducts. We investigated whether ERCC1 gene polymorphism has an effect on the response to chemotherapy and survival in 43 patients with NSCLC treated with platinum-based chemotherapy. ERCC1 19007 T>C SNPs were assessed using a PCR-RFLP methods in DNA isolated from peripheral blood lymphocytes. Disease control occurred significantly (p = 0.045) more frequently in patients with CC or CT genotype compared to patients with TT genotype. Median PFS and OS for CC homozygous were 4 and 10.5 months, 4 and 12.5 months for CT heterozygous, but only 0.3 and 1.5 months for TT homozygous patients, respectively. The probability of PFS was significantly higher (HR = 0.438, 95% CI: 0.084–0.881, p = 0.03) and probability of OS was insignificantly
higher (HR = 0.503, 95% CI: 0.129–1.137, p = 0.084) in patients with CC or CT genotype than in patients with TT genotype. Uncommon TT genotype of ERCC1 19007 T>C polymorphism could predict poor response and shortening of progression free survival in NSCLC patients treated with platinum-based I-line chemotherapy. The analysis of this polymorphism may serve as a promising tool in the qualification of advanced NSCLC patients for appropriate chemotherapy.

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Keywords

ERCC1; single-nucleotide polymorphism; non-small cell lung cancer staging; platinum-based chemotherapy

About this article
Title

Predictive value of ERCC1 single-nucleotide polymorphism in patients receiving platinum-based chemotherapy for locally-advanced and advanced non-small cell lung cancer — a pilot study

Journal

Folia Histochemica et Cytobiologica

Issue

Vol 50, No 1 (2012)

Article type

Original paper

Pages

80-86

Published online

2012-04-25

Page views

2423

Article views/downloads

2775

DOI

10.5603/FHC.2012.0011

Bibliographic record

Folia Histochem Cytobiol 2012;50(1):80-86.

Keywords

ERCC1
single-nucleotide polymorphism
non-small cell lung cancer staging
platinum-based chemotherapy

Authors

Paweł Krawczyk
Kamila Wojas-Krawczyk
Radosław Mlak
Tomasz Kucharczyk
Beata Biernacka
Janusz Milanowski

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