open access

Vol 48, No 4 (2010)
ORIGINAL PAPERS
Published online: 2011-04-12
Submitted: 2011-12-19
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Cytotoxicity and apoptotic effects of nickel oxide nanoparticles in cultured HeLa cells.

Kezban Ada, Mustafa Turk, Serpil Oguztuzun, Murat Kilic, Mehmet Demirel, Nisa Tandogan, Ertan Ersayar, Ozturk Latif
DOI: 10.2478/v10042-010-0045-8
·
Folia Histochem Cytobiol 2010;48(4):524-529.

open access

Vol 48, No 4 (2010)
ORIGINAL PAPERS
Published online: 2011-04-12
Submitted: 2011-12-19

Abstract

The aim of this study was to observe the cytotoxicity and apoptotic effects of nickel oxide nanoparticles on human cervix epithelioid carcinoma cell line (HeLa). Nickel oxide precursors were synthesized by an nickel sulphate-excess urea reaction in boiling aqueous solution. The synthesized NiO nanoparticles (<200 nm) were investigated by X-ray diffraction analysis and transmission electron microscopy techniques. For cytotoxicity experiments, HeLa cells were incubated in 50-500 Îźg/mL NiO for 2, 6, 12 and 16 hours. The viable cells were counted with a haemacytometer using light microscopy. The cytotoxicity was observed low in 50-200 Îźg/mL concentration for 16 h, but high in 400-500 Îźg/mL concentration for 2-6 h. HeLa cells' cytoplasm membrane was lysed and detached from the well surface in 400 Îźg/mL concentration NiO nanoparticles. Double staining and M30 immunostaining were performed to quantify the number of apoptotic cells in culture on the basis of apoptotic cell nuclei scores. The apoptotic effect was observed 20% for 16 h incubation.

Abstract

The aim of this study was to observe the cytotoxicity and apoptotic effects of nickel oxide nanoparticles on human cervix epithelioid carcinoma cell line (HeLa). Nickel oxide precursors were synthesized by an nickel sulphate-excess urea reaction in boiling aqueous solution. The synthesized NiO nanoparticles (<200 nm) were investigated by X-ray diffraction analysis and transmission electron microscopy techniques. For cytotoxicity experiments, HeLa cells were incubated in 50-500 Îźg/mL NiO for 2, 6, 12 and 16 hours. The viable cells were counted with a haemacytometer using light microscopy. The cytotoxicity was observed low in 50-200 Îźg/mL concentration for 16 h, but high in 400-500 Îźg/mL concentration for 2-6 h. HeLa cells' cytoplasm membrane was lysed and detached from the well surface in 400 Îźg/mL concentration NiO nanoparticles. Double staining and M30 immunostaining were performed to quantify the number of apoptotic cells in culture on the basis of apoptotic cell nuclei scores. The apoptotic effect was observed 20% for 16 h incubation.
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About this article
Title

Cytotoxicity and apoptotic effects of nickel oxide nanoparticles in cultured HeLa cells.

Journal

Folia Histochemica et Cytobiologica

Issue

Vol 48, No 4 (2010)

Pages

524-529

Published online

2011-04-12

DOI

10.2478/v10042-010-0045-8

Bibliographic record

Folia Histochem Cytobiol 2010;48(4):524-529.

Authors

Kezban Ada
Mustafa Turk
Serpil Oguztuzun
Murat Kilic
Mehmet Demirel
Nisa Tandogan
Ertan Ersayar
Ozturk Latif

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