Cytotoxicity and apoptotic effects of nickel oxide nanoparticles in cultured HeLa cells.

Kezban Ada; Mustafa Turk; Serpil Oguztuzun; Murat Kilic; Mehmet Demirel; Nisa Tandogan; Ertan Ersayar; Ozturk Latif

doi:10.2478/v10042-010-0045-8

Vol 48, No 4 (2010)

Original paper

Submitted: 2011-12-19

Published online: 2011-04-12

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Cytotoxicity and apoptotic effects of nickel oxide nanoparticles in cultured HeLa cells.

Kezban Ada, Mustafa Turk, Serpil Oguztuzun, Murat Kilic, Mehmet Demirel, Nisa Tandogan, Ertan Ersayar, Ozturk Latif

DOI: 10.2478/v10042-010-0045-8

Folia Histochem Cytobiol 2010;48(4):524-529.

Vol 48, No 4 (2010)

ORIGINAL PAPERS

Submitted: 2011-12-19

Published online: 2011-04-12

Abstract

The aim of this study was to observe the cytotoxicity and apoptotic effects of nickel oxide nanoparticles on human cervix epithelioid carcinoma cell line (HeLa). Nickel oxide precursors were synthesized by an nickel sulphate-excess urea reaction in boiling aqueous solution. The synthesized NiO nanoparticles (<200 nm) were investigated by X-ray diffraction analysis and transmission electron microscopy techniques. For cytotoxicity experiments, HeLa cells were incubated in 50-500 Îźg/mL NiO for 2, 6, 12 and 16 hours. The viable cells were counted with a haemacytometer using light microscopy. The cytotoxicity was observed low in 50-200 Îźg/mL concentration for 16 h, but high in 400-500 Îźg/mL concentration for 2-6 h. HeLa cells' cytoplasm membrane was lysed and detached from the well surface in 400 Îźg/mL concentration NiO nanoparticles. Double staining and M30 immunostaining were performed to quantify the number of apoptotic cells in culture on the basis of apoptotic cell nuclei scores. The apoptotic effect was observed 20% for 16 h incubation.