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Published online: 2021-05-21
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Therapeutic hypothermia: a powerful tool in preventing ischemia-reperfusion-injury in rat hearts

Małgorzata Wojciechowska12, Magdalena Kleszczewska1, Anita Trojanowska1, Tomasz Ciesielski1, Sonia Borodzicz-Jażdżyk1, Katarzyna Czarzasta1, Liana Puchalska1, Maciej Zarębiński2, Agnieszka Cudnoch-Jędrzejewska1
DOI: 10.5603/FC.a2021.0032
Affiliations
  1. Department of Experimental and Clinical Physiology, Laboratory of Centre for Preclinical Research, Medical University of Warsaw, Warsaw, Poland, Poland
  2. Independent Public Specialist Western Hospital John Paul II, Invasive Cardiology Unit, Grodzisk Mazowiecki, Poland, Poland

open access

Ahead of print
Original Papers
Published online: 2021-05-21

Abstract

Introduction. The aim of the experiment was to find the relationship between the extent of myocardial infarction and the internal body temperature of the rats. Our second goal was to investigate if mild hypothermia applied during myocardial ischemia and reperfusion brings cardioprotective effects. Materials and methods. The study was performed in vivo on rat hearts. Myocardial infarction was induced by 30 minutes of the left anterior descending artery occlusion followed by 60 min reperfusion. Thirteen rats were assigned to a group depending on the internal body temperature (t) ≤ 35.5°C (n = 5), 35.6 ≥ t ≥ 37.5°C (n = 4) and t ≥ 37.6°C (n = 4). The desired temperature was achieved during pre-ischemic procedures. The presence of the infarction scar and its size were assessed in planimetry. Infraction size was calculated as the infarct area relative to the area at risk (IA/RA). Results. The incidence of infarction scar in the groups of rats with body temperature ≥ 35.6°C was significantly higher (p < 0.01) compared to the group with the temperature ≤ 35.5°C. There was statistically significant positive correlation (r = 0.787, p < 0.01) between IA/RA and the internal body temperature of the rats. Conclusions. Any reduction in the body temperature during myocardial ischemia and reperfusion seems to be beneficial for the rat’s myocardium. Moreover, hypothermia ≤ 35.5ºC applied during ischemia and reperfusion fully prevents from myocardial infarction. We believe that there is a great need to intensify research on therapeutic hypothermia in humans, so that this method of treatment could be finally used in the course of myocardial infarction.  

Abstract

Introduction. The aim of the experiment was to find the relationship between the extent of myocardial infarction and the internal body temperature of the rats. Our second goal was to investigate if mild hypothermia applied during myocardial ischemia and reperfusion brings cardioprotective effects. Materials and methods. The study was performed in vivo on rat hearts. Myocardial infarction was induced by 30 minutes of the left anterior descending artery occlusion followed by 60 min reperfusion. Thirteen rats were assigned to a group depending on the internal body temperature (t) ≤ 35.5°C (n = 5), 35.6 ≥ t ≥ 37.5°C (n = 4) and t ≥ 37.6°C (n = 4). The desired temperature was achieved during pre-ischemic procedures. The presence of the infarction scar and its size were assessed in planimetry. Infraction size was calculated as the infarct area relative to the area at risk (IA/RA). Results. The incidence of infarction scar in the groups of rats with body temperature ≥ 35.6°C was significantly higher (p < 0.01) compared to the group with the temperature ≤ 35.5°C. There was statistically significant positive correlation (r = 0.787, p < 0.01) between IA/RA and the internal body temperature of the rats. Conclusions. Any reduction in the body temperature during myocardial ischemia and reperfusion seems to be beneficial for the rat’s myocardium. Moreover, hypothermia ≤ 35.5ºC applied during ischemia and reperfusion fully prevents from myocardial infarction. We believe that there is a great need to intensify research on therapeutic hypothermia in humans, so that this method of treatment could be finally used in the course of myocardial infarction.  

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Keywords

ST-elevation myocardial infarction, internal body temperature, cardioprotection

About this article
Title

Therapeutic hypothermia: a powerful tool in preventing ischemia-reperfusion-injury in rat hearts

Journal

Folia Cardiologica

Issue

Ahead of print

Article type

Original paper

Published online

2021-05-21

DOI

10.5603/FC.a2021.0032

Keywords

ST-elevation myocardial infarction
internal body temperature
cardioprotection

Authors

Małgorzata Wojciechowska
Magdalena Kleszczewska
Anita Trojanowska
Tomasz Ciesielski
Sonia Borodzicz-Jażdżyk
Katarzyna Czarzasta
Liana Puchalska
Maciej Zarębiński
Agnieszka Cudnoch-Jędrzejewska

References (21)
  1. Hausenloy DJ, Garcia-Dorado D, Bøtker HE, et al. Novel targets and future strategies for acute cardioprotection: position paper of the European Society of Cardiology Working Group on Cellular Biology of the Heart. Cardiovasc Res. 2017; 113(6): 564–585.
  2. Downey JM. Measuring infarct size by the tetrazolium method. https://www.southalabama.edu/ishr/help/ttc/ (Feruary 7, 2021).
  3. Kohlhauer M, Pell VR, Burger N, et al. Protection against cardiac ischemia-reperfusion injury by hypothermia and by inhibition of succinate accumulation and oxidation is additive. Basic Res Cardiol. 2019; 114(3): 18.
  4. Kanemoto S, Matsubara M, Noma M, et al. Mild hypothermia to limit myocardial ischemia-reperfusion injury: importance of timing. Ann Thorac Surg. 2009; 87(1): 157–163.
  5. Hao L, Jeonghyun P, Yinlun W, et al. Therapeutic hypothermia improves cardiac function after successful resuscitation from cardiac arrest in a rat model of myocardial infarction. Circulation. 2016; 134: A11335.
  6. Knoop B, Naguib D, Dannenberg L, et al. Cardioprotection by very mild hypothermia in mice. Cardiovasc Diagn Ther. 2019; 9(1): 64–67.
  7. Shi J, Dai W, Kloner RA. Therapeutic hypothermia reduces the inflammatory response following ischemia/reperfusion injury in rat hearts. Ther Hypothermia Temp Manag. 2017; 7(3): 162–170.
  8. Lindsey ML, Bolli R, Canty JM, et al. Guidelines for experimental models of myocardial ischemia and infarction. Am J Physiol Heart Circ Physiol. 2018; 314(4): H812–H838.
  9. Chien GL, Wolff RA, Davis RF, et al. "Normothermic range" temperature affects myocardial infarct size. Cardiovasc Res. 1994; 28(7): 1014–1017.
  10. Wood T, Osredkar D, Puchades M, et al. Treatment temperature and insult severity influence the neuroprotective effects of therapeutic hypothermia. Sci Rep. 2016; 6: 23430.
  11. Karcioglu O, Topacoglu H, Dikme O, et al. A systematic review of safety and adverse effects in the practice of therapeutic hypothermia. Am J Emerg Med. 2018; 36(10): 1886–1894.
  12. Götberg M, Olivecrona GK, Koul S, et al. A pilot study of rapid cooling by cold saline and endovascular cooling before reperfusion in patients with ST-elevation myocardial infarction. Circ Cardiovasc Interv. 2010; 3(5): 400–407.
  13. Erlinge D, Götberg M, Lang I, et al. Rapid endovascular catheter core cooling combined with cold saline as an adjunct to percutaneous coronary intervention for the treatment of acute myocardial infarction. The CHILL-MI trial: a randomized controlled study of the use of central venous catheter core cooling combined with cold saline as an adjunct to percutaneous coronary intervention for the treatment of acute myocardial infarction. J Am Coll Cardiol. 2014; 63(18): 1857–1865.
  14. Testori C, Beitzke D, Mangold A, et al. Out-of-hospital initiation of hypothermia in ST-segment elevation myocardial infarction: a randomised trial. Heart. 2019; 105(7): 531–537.
  15. Dae M, O'Neill W, Grines C, et al. Effects of endovascular cooling on infarct size in ST-segment elevation myocardial infarction: A patient-level pooled analysis from randomized trials. J Interv Cardiol. 2018; 31(3): 269–276.
  16. Villablanca PA, Rao G, Briceno DF, et al. Therapeutic hypothermia in ST elevation myocardial infarction: a systematic review and meta-analysis of randomised control trials. Heart. 2016; 102(9): 712–719.
  17. Noc M, Erlinge D, Neskovic AN, et al. COOL AMI EU pilot trial: a multicentre, prospective, randomised controlled trial to assess cooling as an adjunctive therapy to percutaneous intervention in patients with acute myocardial infarction. EuroIntervention. 2017; 13(5): e531–e539.
  18. Wojciechowska M, Wątroba M, Ciużyńska G, et al. Ischaemic heart preconditioning in rats with adjuvant-induced arthritis. Kardiol Pol. 2013; 71(8): 839–844.
  19. Schwarz ER, Somoano Y, Hale SL, et al. What is the required reperfusion period for assessment of myocardial infarct size using triphenyltetrazolium chloride staining in the rat? . J Thromb Thrombolysis. 2000; 10(2): 181–187.
  20. Ferrera R, Benhabbouche S, Bopassa JC, et al. One hour reperfusion is enough to assess function and infarct size with TTC staining in Langendorff rat model. Cardiovasc Drugs Ther. 2009; 23(4): 327–331.
  21. Mou Y, Wilgenburg BJ, Lee Yj, et al. A method for hypothermia-induction and maintenance allows precise body and brain temperature control in mice. J Neurosci Methods. 2013; 213(1): 1–5.

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