The association of A1166C gene polymorphism of angiotensin receptors with the parameters of central pulse wave in normotensive persons and patients with hypertension

Streszczenie

Introduction. Available information about the impact of the polymorphic structure of type 1 angiotensin receptor (ATR1) gene on the central pulse wave is scarce and contradictory. The aim of the study was to establish a possible association of A1166C polymorphism of ATR1 gene with the pulse wave parameters obtained by applanation tonometry.

Material and metods. We examined male and female persons aged 25 to 76 years. They were divided into two groups: the basic consisting of 150 hypertensive patients and the control group presented by 128 individuals with normal blood pressure. In addition to the general clinical exam three-fold measurements of systolic and diastolic blood pressure (systolic blood pressure [SBP] and diastolic blood pressure [DBP], respectively) with the automatic blood pressure monitor Omron M3 were provided mandatory. The structure of the polymorphic receptor gene ATR1 was defined in all participants by polymerase chain reaction on the thermocycler PHC (Techne, UK). Pulse wave analysis was provided with applanation tonometry. For this purpose we used Sphygmocor XCEL equipment (AtCor Medical, Australia).

Results. Analysis of mean SBP and DBP in dependence on ATR1 genotype and existence of arterial hypertension showed that there was a tendency to rise with increasing number of C alleles, but statistical significance of the trend had been confirmed by ANOVA only for SBP in patients with hypertension. Also it was shown a significant difference in central diastolic blood pressure (CDBP) and pulse pressure augmentation (PPA) in hypertensive patients compared with normotensive. It was found a tendency to decrease CDBP and PPA with increasing number of C alleles. The subjects of the control group demonstrated the same trend of PPA as in the group of hypertensive patients.

Conclusion. Thus, we established the association between decrease of PPA and increase of C alleles number in the ATR1 gene.