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Effects of ethephon on serum levels of sex hormone, apoptosis, and cell cycle of ovaries in mice
Affiliations- Department of Occupational and Environmental Health, School of Public Health, Guangxi Medical University, Nanning, Guangxi, China
- Preventive Medicine Experimental Teaching Centre, School of Public Health, Jilin University, Changchun, Jilin, China
open access
Abstract
Introduction: The effects of ethephon on the reproductive systems of mammalian females are still ambiguous. This study was conducted to evaluate the toxic effects of ethephon on the female reproductive system.
Material and methods: Forty female C57 mice were used as experimental subjects and evenly divided into 8 groups, which were fed with mixed ethephon (0, 107.3, 214.5, and 429 mg/kg bw/day) and pure water. After 20 and 40 days of gavage, the mice were weighed and individual organ coefficients of the ovaries were measured. Enzyme-linked immunoassay was used to detect the serum levels of serum sex hormones. The cell cycle distribution and rate of apoptosis of mouse ovarian tissues were examined using flow cytometry.
Results: Ethephon intoxication significantly decreased serum levels of progesterone (P) and oestradiol (E2) and increased the serum levels of luteinizing hormone (LH). The serum levels of follicle-stimulating hormone (FSH) decreased and then increased over time. In addition, ethephon significantly inhibited the apoptosis rate in the ovary and caused G0/G1 and G2/M arrest.
Conclusion: These results indicate that prolonged exposure to ethephon may have negative effects on the female reproductive system.
Abstract
Introduction: The effects of ethephon on the reproductive systems of mammalian females are still ambiguous. This study was conducted to evaluate the toxic effects of ethephon on the female reproductive system.
Material and methods: Forty female C57 mice were used as experimental subjects and evenly divided into 8 groups, which were fed with mixed ethephon (0, 107.3, 214.5, and 429 mg/kg bw/day) and pure water. After 20 and 40 days of gavage, the mice were weighed and individual organ coefficients of the ovaries were measured. Enzyme-linked immunoassay was used to detect the serum levels of serum sex hormones. The cell cycle distribution and rate of apoptosis of mouse ovarian tissues were examined using flow cytometry.
Results: Ethephon intoxication significantly decreased serum levels of progesterone (P) and oestradiol (E2) and increased the serum levels of luteinizing hormone (LH). The serum levels of follicle-stimulating hormone (FSH) decreased and then increased over time. In addition, ethephon significantly inhibited the apoptosis rate in the ovary and caused G0/G1 and G2/M arrest.
Conclusion: These results indicate that prolonged exposure to ethephon may have negative effects on the female reproductive system.
Keywords
ethephon; female mice; cell cycle; apoptosis; serum sex hormone
About this articleTitle
Effects of ethephon on serum levels of sex hormone, apoptosis, and cell cycle of ovaries in mice
Journal
Issue
Article type
Original paper
Pages
346-352
Published online
2022-04-13
Page views
4900
Article views/downloads
580
DOI
Pubmed
Bibliographic record
Endokrynol Pol 2022;73(2):346-352.
Keywords
ethephon
female mice
cell cycle
apoptosis
serum sex hormone
Authors
Hongyuan Huang
Xiaoqiu Zhao
Xin Shi
Qiyue Tan
Ruizhi Zhang
Mei Yue
Rongshuang Ma
Qiang Chen
Shuhua Zhao
Li Yang
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