open access

Vol 70, No 3 (2019)
Original Paper
Published online: 2019-01-30
Submitted: 2018-10-05
Accepted: 2018-12-10
Get Citation

Expression of α-Klotho protein in human thyroid cancers — an immunohistochemical study

Marek Pawlikowski, Hanna Pisarek, Magdalena Borkowska, Katarzyna Winczyk
DOI: 10.5603/EP.a2019.0004
·
Pubmed: 30699231
·
Endokrynologia Polska 2019;70(3):237-240.

open access

Vol 70, No 3 (2019)
Original Paper
Published online: 2019-01-30
Submitted: 2018-10-05
Accepted: 2018-12-10

Abstract

Introduction: The α-Klotho protein was discovered as a gene controlling the process of aging, but further studies indicated that it also plays the role of a tumour suppressor. Although numerous studies were performed on the role of the α-Klotho gene and protein in neoplasia, the data on α-Klotho protein expression in thyroid cancers are very scarce. Our study presents the immunohistochemical investigation of α-Klotho expression in benign and malignant thyroid tumours.

Material and methods: The material included samples of benign (nodular hyperplasia, follicular adenoma), differentiated (follicular and papillary) cancers and aggressive thyroid cancers of low differentiation grade. The samples were immunostained using two different monoclonal anti-α-Klotho antibodies.

Results: From the two antibodies used in this study, one (EPR6856) reacted probably with the soluble form of Klotho and immunostained mostly the colloid filling thyroid follicles and intravascular or extravascular serum deposits. The other (A-9 antibody) immunostained the follicular epithelium in benign thyroid lesions as well as the epithelial tumoural cells in differentiated thyroid (follicular and papillary cancers). In the thyroid cancers of high malignancy, the immunostaining with A-9 anti-α-Klotho antibody was (except in one case) negative or very weak.

Conclusion: Our results indicate that lowered expression of a- Klotho is involved in the process of thyroid neoplasia.

Abstract

Introduction: The α-Klotho protein was discovered as a gene controlling the process of aging, but further studies indicated that it also plays the role of a tumour suppressor. Although numerous studies were performed on the role of the α-Klotho gene and protein in neoplasia, the data on α-Klotho protein expression in thyroid cancers are very scarce. Our study presents the immunohistochemical investigation of α-Klotho expression in benign and malignant thyroid tumours.

Material and methods: The material included samples of benign (nodular hyperplasia, follicular adenoma), differentiated (follicular and papillary) cancers and aggressive thyroid cancers of low differentiation grade. The samples were immunostained using two different monoclonal anti-α-Klotho antibodies.

Results: From the two antibodies used in this study, one (EPR6856) reacted probably with the soluble form of Klotho and immunostained mostly the colloid filling thyroid follicles and intravascular or extravascular serum deposits. The other (A-9 antibody) immunostained the follicular epithelium in benign thyroid lesions as well as the epithelial tumoural cells in differentiated thyroid (follicular and papillary cancers). In the thyroid cancers of high malignancy, the immunostaining with A-9 anti-α-Klotho antibody was (except in one case) negative or very weak.

Conclusion: Our results indicate that lowered expression of a- Klotho is involved in the process of thyroid neoplasia.

Get Citation

Keywords

α-Klotho; thyroid malignancies; immunohistochemistry

About this article
Title

Expression of α-Klotho protein in human thyroid cancers — an immunohistochemical study

Journal

Endokrynologia Polska

Issue

Vol 70, No 3 (2019)

Pages

237-240

Published online

2019-01-30

DOI

10.5603/EP.a2019.0004

Pubmed

30699231

Bibliographic record

Endokrynologia Polska 2019;70(3):237-240.

Keywords

α-Klotho
thyroid malignancies
immunohistochemistry

Authors

Marek Pawlikowski
Hanna Pisarek
Magdalena Borkowska
Katarzyna Winczyk

References (16)
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