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open access

Vol 69, No 3 (2018)
Original paper
Submitted: 2017-10-17
Accepted: 2017-12-22
Published online: 2018-05-09
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Th17 and IL-17 exhibit higher levels in osteonecrosis of the femoral head and have a positive correlation with severity of pain

Debo Zou12, Kaining Zhang1, Yun Yang1, Yanjun Ren1, Lei Zhang1, Xing Xiao1, Haoxuan Zhang1, Shuai Liu1, Jingkun Li1
DOI: 10.5603/EP.a2018.0031
·
Pubmed: 29952419
·
Endokrynol Pol 2018;69(3):283-290.
Affiliations
  1. Department of Orthopaedic Surgery, Shandong Provincial Qianfoshan Hospital, Shandong University, No. 16766, Jingshi Road, Jinan, Shandong 250014, People’s Republic of China, Jinan, China
  2. Shandong University, School of Medicine, Jinan 250012, Shandong, P.R. China, Jinan, China

open access

Vol 69, No 3 (2018)
Original Paper
Submitted: 2017-10-17
Accepted: 2017-12-22
Published online: 2018-05-09

Abstract

Objective: Synovitis associated with osteonecrosis of the femoral head (ONFH) is responsible for several clinical symptoms. However, the mechanisms underlying synovitis and the inflammatory environment remain unclear. This study analyzed the proinflammatory mediation expression of IL-17 and Th17, which perform key functions in regulating inflammatory processes in the inflamed synovium and peripheral blood in ONFH. Methods: Synovial fluid from the hips of 23 patients and 5 controls was collected during surgery, and peripheral blood samples were obtained from 34 patients and 9 controls. The expression of IL-17 in the synovium was detected by immunohistochemistry, and the levels of Th17 and IL-17 in the blood were measured by flow cytometry and ELISA. Pain assessment was performed for all the patients and controls. Results: An inflamed synovium was characterized by increased leukocyte infiltration and IL-17 expression in comparison with the control. Preoperative levels of Th17 and IL-17 were significantly higher in the peripheral blood of the ONFH group than those in the controls. The symptoms were also positively correlated with the Th17 levels of the ONFH patients. Conclusion: Th17 cells were recruited to an inflamed synovium, and inflammatory cytokine IL-17 was expressed at an increased level in the hip synovium of ONFH patients, which possibly contributed to clinical syndrome development. Overall, this study will help in identifying new therapeutic strategies for ONFH, especially the targeting of IL-17 to decrease inflammation and pain.

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Abstract

Objective: Synovitis associated with osteonecrosis of the femoral head (ONFH) is responsible for several clinical symptoms. However, the mechanisms underlying synovitis and the inflammatory environment remain unclear. This study analyzed the proinflammatory mediation expression of IL-17 and Th17, which perform key functions in regulating inflammatory processes in the inflamed synovium and peripheral blood in ONFH. Methods: Synovial fluid from the hips of 23 patients and 5 controls was collected during surgery, and peripheral blood samples were obtained from 34 patients and 9 controls. The expression of IL-17 in the synovium was detected by immunohistochemistry, and the levels of Th17 and IL-17 in the blood were measured by flow cytometry and ELISA. Pain assessment was performed for all the patients and controls. Results: An inflamed synovium was characterized by increased leukocyte infiltration and IL-17 expression in comparison with the control. Preoperative levels of Th17 and IL-17 were significantly higher in the peripheral blood of the ONFH group than those in the controls. The symptoms were also positively correlated with the Th17 levels of the ONFH patients. Conclusion: Th17 cells were recruited to an inflamed synovium, and inflammatory cytokine IL-17 was expressed at an increased level in the hip synovium of ONFH patients, which possibly contributed to clinical syndrome development. Overall, this study will help in identifying new therapeutic strategies for ONFH, especially the targeting of IL-17 to decrease inflammation and pain.

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Keywords

T helper 17 lymphocyte; IL-17; osteonecrosis of femoral head; inflammation; pain; visual analogue scale

About this article
Title

Th17 and IL-17 exhibit higher levels in osteonecrosis of the femoral head and have a positive correlation with severity of pain

Journal

Endokrynologia Polska

Issue

Vol 69, No 3 (2018)

Article type

Original paper

Pages

283-290

Published online

2018-05-09

Page views

3028

Article views/downloads

1313

DOI

10.5603/EP.a2018.0031

Pubmed

29952419

Bibliographic record

Endokrynol Pol 2018;69(3):283-290.

Keywords

T helper 17 lymphocyte
IL-17
osteonecrosis of femoral head
inflammation
pain
visual analogue scale

Authors

Debo Zou
Kaining Zhang
Yun Yang
Yanjun Ren
Lei Zhang
Xing Xiao
Haoxuan Zhang
Shuai Liu
Jingkun Li

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