Endokrynologia Polska 6/2013-Pancreatic neuroendocrine neoplasms — management guidelines (recommended by the Polish Network of Neuroendocrine Tumours)

SZKOLENIE PODYPLOMOWE/POSTGRADUATE EDUCATION

Pancreatic neuroendocrine neoplasms — management guidelines (recommended by the Polish Network of Neuroendocrine Tumours)

Nowotwory neuroendokrynne trzustki — zasady postępowania (rekomendowane przez Polską Sieć Guzów Neuroendokrynnych)

Beata Kos-Kudła1, Alicja Hubalewska-Dydejczyk2, Katarzyna Kuśnierz3, Paweł Lampe3, Bogdan Marek4, Anna Nasierowska-Guttmejer5, Ewa Nowakowska-Duława6, Joanna Pilch-Kowalczyk7, Anna Sowa-Staszczak8, Violetta Rosiek1

and other participants of the Consensus Conference (affiliations at the end of this section)

Elżbieta Andrysiak-Mamos, Tomasz Bednarczuk, Jolanta Blicharz-Dorniak, Marek Bolanowski, Andrzej Cichocki, Jarosław B. Ćwikła, Andrzej Deptała, Wanda Foltyn, Daria Handkiewicz-Junak, Marek Hartleb, Michał Jarząb, Arkadiusz Jeziorski, Dariusz Kajdaniuk, Grzegorz Kamiński, Aldona Kowalska, Robert Król, Leszek Królicki, Jolanta Kunikowska, Dariusz Lange, Anna Lewczuk, Magdalena Londzin-Olesik, Przemysław Majewski, Gabriela Mełeń-Mucha, Andrzej Nowak, Waldemar Patkowski, Marek Ruchała, Sławomir Rudzki, Philippe Ruszniewski, Grażyna Rydzewska, Teresa Starzyńska, Katarzyna Steinhof-Radwańska, Janusz Strzelczyk, Wojciech Zajęcki, Piotr Zdunowski, Anna Zemczak

 

1Division of Endocrinology, Department of Pathophysiology and Endocrinology, Medical University of Silesia, Katowice, Poland

2Chair and Department of Endocrinology, Jagiellonian University Collegium Medicum, Krakow, Poland

3Department of Gastrointestinal Surgery, Medical University of Silesia, Katowice, Poland

4Division of Pathophysiology, Department of Pathophysiology and Endocrinology, Medical University of Silesia, Katowice, Poland

5Department of Pathomorphology, Central Clinical Hospital of the Ministry of Internal Affairs in Warsaw, Warsaw, Jan Kochanowski University, Kielce, Poland

6Department of Gastroenterology and Hepatology, Medical University of Silesia, Katowice, Poland

7Department of Radiology, Medical University of Silesia, Katowice, Poland

8Nuclear Medicine Unit, The University Hospital, Krakow, Poland

Prof. Beata Kos-Kudła M.D., Ph.D., Division of Endocrinology, Department of Pathophysiology and Endocrinology, Medical University of Silesia, Ceglana St. 35, 40–952 Katowice, Poland, tel./fax: +48 32 358 13 66, e-mail: endoklin@sum.edu.pl

 

Abstract

We present revised diagnostic and therapeutic guidelines for the management of pancreatic neuroendocrine neoplasms (PNENs) proposed by the Polish Network of Neuroendocrine Tumours. These guidelines refer to biochemical (determination of specific and nonspecific neuroendocrine markers) and imaging diagnostics (EUS, CT, MR, and radioisotope examination with a 68Ga or 99Tc labelled somatostatin analogue).

A histopathological diagnostic, which determines the further management of patients with PNENs, must be necessarily confirmed by immunohistochemical tests. PNENs therapy requires collaboration between a multidisciplinary team of specialists experienced in the management of these neoplasms. Surgery is the basic form of treatment. Medical therapy requires a multidirectional procedure, and therefore the rules of biotherapy, peptide receptor radionuclide therapy, chemotherapy and molecular targeted therapy are discussed.

(Endokrynol Pol 2013; 64 (6): 459–479)

 

Key words: pancreatic neuroendocrine neoplasms; functioning; non-functioning; diagnosis; therapy; guidelines

 

Streszczenie

W niniejszej publikacji przedstawiono zaktualizowane zalecenia dotyczące diagnostyczno-terapeutycznego postępowania w nowotworach neuroendokrynnych trzustki (PNEN) zaproponowane przez Polską Sieć Guzów Neuroendokrynnych.

Dotyczą one diagnostyki biochemicznej (oznaczanie specyficznych i niespecyficznych markerów neuroendokrynnych) i lokalizacyjnej (z uwzględnieniem EUS, CT, MR, scyntygrafii receptorów somatostatynowych z użyciem analogów znakowanych 68Ga lub 99Tc). Duże znaczenie ma rozpoznanie histopatologiczne, które determinuje dalsze postępowanie z chorymi na PNEN i musi być potwierdzone badaniem immunohistochemicznym. Terapia PNEN wymaga współpracy wielodyscyplinarnej grupy doświadczonych specjalistów zajmujących się nowotworami neuroendokrynnymi. Leczenie chirurgiczne jest postawową metodą postępowania. Dalsza terapia wymaga wielokierunkowego działania, dlatego omówiono zasady bioterapii, leczenia izotopowego, chemioterapii oraz celowanego leczenia molekularnego.

(Endokrynol Pol 2013; 64 (6): 459–479)

 

Słowa kluczowe: nowotwory neuroendokrynne trzustki; czynne hormonalnie; nieczynne hormonalnie; diagnostyka; terapia; zalecenia

1. Epidemiology, prognosis/survival

1.1. Pancreatic neuroendocrine neoplasms (excluding gastrinomas)

The incidence of pancreatic neuroendocrine neoplasms (PNENs) is approximately 0.32/100,000/year. PNENs account for approximately 30% of all gastroentreopancreatic neoplasms (GEP NENs). 45–60% of PNENs (in some registers up to 90%) are non-functioning. Despite the lack of symptoms of hormonal hypersecretion, they demonstrate the ability to produce certain substances, e.g. pancreatic polypeptide, chromogranin A, neuron-specific enolase, β-hCG subunit, calcitonin, neurotensin and other peptides. On the other hand, 40–55% of PNENs demonstrate excessive hormonal activity (functioning tumours), which is reflected in corresponding clinical symptoms [1–3].

Functioning PNENs include [1, 4]:

— insulinoma — secreting insulin,

— gastrinoma — secreting gastrin,

— glucagonoma — secreting glucagon,

— VIPoma — secreting vasoactive intestinal peptide,

— PPoma — secreting pancreatic polypeptide (often classified as a non-functioning tumour),

— somatostatinoma — secreting somatostatin.

Very rare functioning PNENs include: CRHoma — secreting CRH — hormone stimulating production of corticotrophin, calcitoninoma — secreting calcitonin and PNENs producing corticotrophin (ACTH), growthhormone-releasing hormone (GHRH), neurotensin, parathyroid hormone-related peptide (PTHrP), rennin, luteinising hormone (LH) and others.

ThemostcommonfunctioningPNENsareinsulinoma and gastrinoma [1, 4] (discussed in detail in the section on gastroduodenal neuroendocrine neoplasms (NENs).

1.2. Clinical characteristics of PNEN

Insulinoma — insulin-secreting pancreatic neoplasm is the most common functioning neuroendocrine tumour of this organ. In approximately 1% of patients, an extrapancreatic location is possible (duodenum, stomach, bile ducts, lungs) [5]. Its incidence rate is estimated at 1–3 cases/1,000,000/year. The highest incidence is observed in the fifth decade of life (between the ages of 40–45), and slightly more often in females (60%). Less than 10% of all tumours are malignant [4]. Insulinoma is usually single, and only 10% of patients have multiple tumours (often in multiple endocrine neoplasia type 1 [MEN1]).In approximately 4–5% they are associated with MEN1 syndrome [4, 5, 7, 8]. Clinical symptoms are due to hypoglycaemia, not the presence of tumour (usually it is no more than 2 cm in diameter). They result from neuroglycopenia: pains and vertigo, blurred vision, double vision, abnormal behaviour, confusion, concentration disorders, retrograde amnesia, drowsiness, hallucinations, delusions and convulsions. In approximately 12% of patients, loss of consciousness occurs with a grand mal seizure. Severe hypoglycaemia may result in death. Decreasedbloodglucoselevelalsocausesincreasedsecretion of catecholic amines, and therefore: paleness, increased perspiration, hand tremor, nausea, palpitations, hunger (often increased body weight) and weakness. Although the hypoglycaemic episodes usually occur several hours after a meal, often in the morning, irregularly and with different duration, in approximately 6% of patients hypoglycaemia can only occur soon after a meal [9]. They may be triggered by physical effort, consumption of ethyl alcohol or a low-calorie diet [4, 6, 7, 10–12].

A positive Whipple’s triad is helpful in diagnosing insulinoma:

  1. Clinical symptoms suggestive of hypoglycaemia;
  2. Decreased blood glucose level (< 40 mg/dL; 2.2mmol/L) measured at the time of the symptoms;
  3. Relief of symptoms after intake of carbohydrates.

Prognosis: in benign tumours — very good; in over 95% of such patients, a surgical procedure results in complete recovery. In patients with distant metastases, mean survival is less than two years. Tumour diameter > 2 cm, Ki-67 > 2%, and various molecular and chromosomal disorders, e.g. loss of 3p or 6q, are factors associated with decreased survival [6–8, 10–13].

Other tumours are classified as rare functioning pancreatic neuroendocrine neoplasms (RF-PNENs). They may occur in the pancreas and in other locations. The clinical symptoms associated with the presence of such neoplasms reflect the action of a hormone secreted by the tumour. In the case of very rare neoplasms, interpretation of the symptoms is often ambiguous [4, 6].

RF-PNENs constitute < 10% of PNENs. In a large number (40–90%) of patients with RF-PNENs, hepatic metastases are present already at diagnosis [4, 6, 14].

VIPoma (Verner-Morrison syndrome, pancreatic cholera, WDHA syndrome): incidence rate 0.05– 0.2/1,000,000/year, malignant neoplasms in 40–70%, in ca.3–6%associatedwithMEN1;location:primarilybody of the pancreas (90%), also sympathetic nervous system, liver, adrenal glands. Symptoms: diarrhoea (90–100%), hypokalaemia (80–100%), dehydration (83%), acidosis, rarely skin reddening, hypercalcaemia, glucose intolerance and functioning gall bladder disorders [4, 15–18].

Glucagonoma: incidence rate 0.01–0.1/1,000,000/year, malignant neoplasms 50–80%; in 1–20% associated with MEN1; location: body of the pancreas; symptoms: necrolytic erythema (67–90%), glucose intolerance (38–87%), weight loss (66–96%), stomatitis, diarrhoea and hypoaminoacidemia [4, 15–18].

Somatostatinoma: very low incidence rate, malignant neoplasms > 70%, in 45% associated with MEN1, location: pancreas (55%), duodenum/small intestine 44%, symptoms: cholelithiasis (65–90%), diabetes (63–90%), diarrhoea (35–90%) and weight loss [4, 15–18].

GHRHoma: unknown incidence rate, malignant neoplasms > 60%, location: pancreas (30%), lungs (54%), small intestine (7%), other (9%), associated with MEN1 in 16%, symptoms: acromegaly [4, 15, 16].

ACTHoma: low incidence, malignant neoplasms > 95%, pancreatic location (4–16%), others — extrapancreatic location, rarely associated with MEN1, symptoms: Cushing’s syndrome [4, 15, 16].

PNEN causing carcinoid syndrome: secretes serotonin or tachykinins, very rare: pancreatic location < 1%, malignant neoplasms 60–88%, rarely associated with MEN1, symptoms: carcinoid syndrome [4, 15, 16].

PTHrPoma: very rarely located in the pancreas, malignant neoplasms 84%, rarely associated with MEN1, symptoms: hypercalcaemic syndrome or, in the case of hepatic metastases — abdominal pain [4, 15, 16].

Prognosis: in RF-PNENs, it depends on the size of the tumour and the presence of distant metastases. The five-year survival rate in the case of advanced disease is estimated at 29–45%. Ki-67 > 2%, distant metastases, chromosomal disorders and presence of cytokeratin-19 are unfavourable prognostics [4, 6, 13, 15, 16, 19].

Non-functioning pancreatic neuroendocrine neoplasms (NF-PNENs) do not cause characteristic symptoms of hormonal hypersecretion. In some tumours, immunohistochemical methods have revealed the presence of various hormonal substances produced by these neoplasms, but not secreted into the blood circulation (or secreted in quantities which do not result in clinical symptoms). Most of them are well-differentiated tumours. Their incidence rate is 1.8/1,000,000/year in females and 2.6/1,000,000/year in males. The frequency of detection increases with age, with the peak incidence in the 6th and 7th decades of life. In 3-53% (mean 19%), they are associated with MEN1 syndrome (the frequency is age-related, being higher in elderly patients) and in 13-17% with von Hippel-Lindau (VHL) syndrom [2, 20–23].

Symptoms: NF-PNENs are usually diagnosed late, when they are of large size, cause pressure on the adjacent organs or invade them, or produce distant metastases [2, 3]. The most common symptoms include: abdominal pain (35–78%), weight loss (20–35%), loss of appetite and vomiting (45%). Less common are internal haemorrhages (4–20%), jaundice (17–50%) or palpable tumours in the abdomen (7–40%) [2, 24–26]. Recent studies by Italian researchers have demonstrated that hepatic metastases are observed in 32% of patients with a newly diagnosed NF-PNEN [27]. This value is significantly lower compared to previous studies (46–73%) [21, 28–30].

Prognosis: The mean survival of patients with NF-PNENs in currently available studies is 38 months, with five-year survival of 43% [2, 21]. The mean survival of patients with distant metastases was approximately 23 months, compared to 70 and 124 months’ survival in the case of a localised disease [2, 21, 31]. The histological grading of the tumour is also an important factor affecting the length of survival [2, 32]. Other unfavourable prognostic factors include: age >40 years, dynamic development of hepatic metastases (increase of 25% of their volume in 6-12 months), and occurrence of osseous metastases [2, 33, 34].

2. Diagnostics

2.1. Biochemical diagnostics

Biochemical diagnostics of hormones and markers secreted by neuroendocrine neoplasms (NENs) may be helpful in three aspects: initial diagnosis of the disease, assessment of treatment efficacy, and prognosis.

2.1.1. Non-functioning pancreatic neuroendocrine neoplasms (NF-PNENs)

Biochemical tests of PNENs can use chromogranin A (CgA), which is a marker of most NENs. On the other hand, the level of chromogranin B (CgB) may be elevated when the level of CgA is within the reference range [2, 35, 36].

NF-PNENs often secrete pancreatic polypeptide (PP). A simultaneous measurement of CgA and PP concentration increases the diagnostic sensitivity of PNENs from 74% to 90%. PP is secreted in large quantities by a significant group of NENs of the entire gastrointestinal tract (50–80% of PNENs).

The following indicators are sometimes used in biochemical diagnostics of non-functioning NENs: neuron specific enolase (NSE) and the β subunit of human chorionic gonadotropin (hCG). NSE is mainly determined in neuroendocrine carcinoma (NEC), if the CgA concentration is normal.

The concentration of certain peptides, mostly insulin, gastrin and PP, increases significantly after meals; it may remain elevated for more than six hours after a meal [4]. Therefore, blood for the assay should be collected only after overnight fasting. For some markers, e.g. CgA, it is not necessary to collect blood under fasted conditions. If blood samples are not collected under fasted conditions, this fact should always be mentioned to ensure the proper interpretation by the laboratory. Moreover, the blood concentrations of all NEN markers, except insulin, are elevated in patients with renal failure, so it is difficult to interpret results in such patients. Among numerous markers assessed in the blood, CgA is a prognostic factor for most NENs [37, 38, 39].

2.1.2. Functioning pancreatic neuroendocrine neoplasms (F-PNENs)

Biochemical diagnostics of all F-PNENs requires the evidence of increased serum concentrations of specific hormonal markers (e.g. gastrin in Zollinger-Ellison syndrome (ZES) or insulin in insulinoma) in combination with clinical symptoms/Laboratory changes indicating the hypersecretion of an appropriate hormone, such as excessive secretion of gastric juice in ZES, hypoglycaemia in insulinoma, etc. [4]. In a great number of sporadic NENs, the type of cells may change and tumours may produce various additional peptides (apart from those specific for the tumour). It is related to worsening of the prognosis, especially when the tumour additionally secretes ACTH [40, 41].

Most insulinomas are ‘benign’ tumours with proper serum CgA levels which increase with metastases.

Insulinoma

The diagnosis of insulinoma is based on the following criteria:

— documented glycaemia ≤ 2.2 mmol/L (≤ 40 mg/dL) and concomitant inadequate concentration of insulin ≥ 6 mU/L (≥ 36 pmol/L);

— C-peptide level ≥ 200 pmol/L;

— proinsulin level ≥ 5 pmol/L.

Interpretation of the above criteria should include drug-induced hypoglycaemia by verifying the serum and/ or urinary levels of sulphonylurea and its metabolites [4].

When diagnosing insulinoma, the 72-hour fasting test is still the gold standard, although some studies report that a 48-hour test may be sufficient. The fasting test is performed in inpatient conditions, with serial measurements of the blood glucose level. Patients with insulinoma usually develop hypoglycaemia within 24 hours. Increased levels of ketones in urine indicate the proper fasting test in healthy people. In 5% of patients, hypoglycaemia may occur after meals [42]. When the symptoms of hypoglycaemia occur and the glucose level in the blood is ≤ 2.2 mmol/L (≤ 40 mg/dL), the blood should be collected for C-peptide, proinsulin and insulin assays. The lack of adequate suppression of insulin in hypoglycaemia confirms the presence of an independently secreting insulinoma-type tumour [4].

In one of the recent studies, the most sensitive criterion for diagnosing insulinoma was the coexistence of elevated proinsulin levels and fasting glycaemia of ≤ 2.5 mmol/L (≤ 45 mg/dL) [4].

Gastrinoma

The biochemical diagnostics of gastrinoma are discussed in the section on gastroduodenal NENs (pp. 444–458).

2.1.3. Rare functioning pancreatic neuroendocrine neoplasms (RF-PNENs)

Biochemical diagnostics of a RF-PNEN includes confirmation of increased serum concentrations of specific biochemical markers, e.g. glucagon is suspected glucagonoma (positive result > 1,000 pg/mL), vasoactive intestinal peptide (positive result > 170 pg/mL), somatostatin (positive result in pancreatic neoplasms location is over 50 times higher than the reference values) [34].

CgA, which is a general marker, can only be used to confirm the presence of a neuroendocrine neoplasm and monitor the course of the disease, but it cannot constitute the basis for the diagnosis of functioning PNENs.

All biochemical tests should be performed during the first visit. Suspected Cushing’s syndrome due to PNEN should be confirmed by 24-hour urine or midnight serum cortisol measurements, or by determination of cortisol concentration in saliva. If necessary, the determination of cortisol with the use of the dexamethasone suppression test should be performed.

The assessment of markers specific for NEN is useful in the diagnosis and monitoring of various neoplasms [43], as set out in Table I. Indications for their determination depend on the clinical presentation of the patient with a PNEN.

Table I. Specific markers for various PNENs (modified according to [34, 43])

Tabela I. Specyficzne markery dla różnych PNEN (zmodyfikowane wg [34, 43])

Tumour type Laboratory tests Expected results
All pancreatic NENs CgA Increased concentration only in metastatic tumours
Non-functioning NENs PP, NSE, hCG Increased concentration
Insulinoma CgA, insulin, glucose,
C-peptide or proinsulin
Inadequate increase in the insulin/glucose concentration ratio
Increased concentration
Gastrinoma Gastrin Increased concentration
Glucagonoma Glucagon, enteroglucagon Increased concentration
VIPoma VIP Increased concentration
Somatostatinoma SST Increased concentration
PPoma PP Increased concentration
MEN1 CgA, gastrin, calcium, PTH, insulin, glucagon, PP, PRL Increased concentration of selected markers

CgA — chromogranin A; CgB — chromogranin B; hCG — human chorionic gonadotropin; 5-HIAA — 5-hydroxyindoleacetic acid; NSE — neuron specific enolase; PP — pancreatic polypeptide; PTH — parathyroid hormone; SST — somatostatin; VIPoma — tumor secreting vasoactive intestinal peptide; PRL — prolactin

2.1.4. Pancreatic neuroendocrine carcinoma (PNEC)

An assessment of the CgA levels and other hormonal markers in this group of PNEC usually produces negative results. NSE may be used as a marker for these neoplasms [34].

 

Minimal consensus on biochemical tests:

Determination of plasma CgA level should be the basic biochemical test in patients with suspected PNENs [44]. In non-functioning NENs, pancreatic polypeptide (PP) can be used for early detection of PNENs in MEN1 and PNEC (especially with low CgA level).

Determination of specific markers (gastrin, insulin, serotonin, VIP, glucagon, etc.) should be performed if the patient presents symptoms suggestive of a hormonal clinical syndrome. Specific dynamic tests are very rarely performed. (*evidence level 3).

2.2. Pathomorphological diagnostics

2.2.1. Pathogenesis

The term ‘pancreatic neuroendocrine neoplasms’ refers to tumours arising from a pluripotent stem cell of the pancreatic ducts with neuroendocrine differentiation. The term ‘islet cell tumour’, frequently used in the past, is incorrect due to NEN histogenesis, as these neoplasms do not arise from pancreatic islets. Pathomorphological diagnostics of NENs is based on the standardised WHO classification. The diagnosis is confirmed by immunohistochemical methods, in order to assess the expression of neuroendocrine markers: chromogranin A and synaptophysin, and the Ki-67/MIB1 proliferation index. Immunohistochemical examination of the hormonal substances produced by pancreatic cells is not sufficient for the diagnosis of functioning or non-functioning tumours [34]. Pancreatic cells may demonstrate immunohistochemical expression of the analysed products even in minimal quantities, without any clinical significance.

Table II. ENETS and TNM UICC/AJCC classification, 2011

Tabela II. Klasyfikacja TNM ENETS i AJCC/UICC, 2011

Feature T according to TNM TNM ENETS TNM AJCC/UICC, 2011
T1 Tumour limited to pancreas, < 2 cm in diameter Tumour limited to pancreas, < 2 cm in diameter
T2 Tumour limited to pancreas, 2–4 cm in diameter Tumour limited to pancreas, > 2 cm in diatemer
T3 Tumour limited to pancreas, > 4 cm in diatemer or invading duodenum or bile tract Tumor invading the adjacent tissues, without invasion of the main vascular trunks (coeliac axis, superior mesenteric artery)
T4 Inavsion of the adjacent argans or the wall of the large vessels Invasion of the wall of large vessels

2.2.2. Diagnostic algorithm

Histopathological diagnostics of PNENs requires an assessment of [45–50]:

— histological type according to the WHO 2010 classification;

— histological grade according to the ENETS/WHO 2010 classification;

— pTNM stage of clinical and pathological advancement (according to ENETS, AJCC/UICC of 2010) (Table II);

— each diagnosis of NEN must be confirmed by immunohistochemical examinations with the use of antibodies against chromogranin A and synaptophysin, and by the Ki-67/MIB1 proliferative activity assessment;

— in certain cases, products secreted by NENs, such as gastrin, insulin or glucagon may be assessed.

These markers are more useful for detection of the metastases of functioning tumours, especially if the original site is unknown. Clinical staging of NENs is presented in Table III.

Classification of NENs according to WHO 2010 and the histological grade of NETs according to the standardised ENETS/WHO 2010 system are presented in the section on general recommendations for the management of GEP NENs (pp. 418–443).

Table III. Clinical staging of PNENs

Tabela III. Stopień klinicznego zaawansowania PNEN

Clinical stage Comments
Stage IA T1 N0 M0
Stage IB T2 N0 M0
Stage IIA T3 N0 M0
Stage IIB T1-3 N1 M0
Stage III T4, any N M0
Stage IV any T, any N, M1

2.2.3. Prognostic factors in the histopathological report

In the histopathological examination, it should be noted that nodules smaller than 5 mm are referred to as microadenoma, and are not considered in the histopathological report. Multiple foci are characteristic for PNENs, especially in MEN1, in over 30% of gastrinoma cases and 13% of insulinoma cases. Therefore, a very careful microscopic assessment of the surgical material, involving cross-sections of the pancreatic parenchyma at 3 to 5 mm intervals, is necessary. In each case, an assessment of resectability is an important prognostic parameter. In order to perform it, it is necessary to evaluate macroscopically and microscopically the surgical margins: transpancreatic, retroperitoneal and radial, created by the posterior wall of the surgical material. Assessment of the vascular and neural invasion is also recommended, as according to certain clinical studies it is associated with lymph nodes metastases and shorter life expectancy. Coagulative necrosis, local or geographic, is another prognostic factor, as it correlates with a high grade of histological malignancy of the tumour.

The morphological picture of the tumour, comprising tumour tissue architecture and characteristics of its cells, is also reflected in the tumour differentiation stage [51, 52]. Under a light microscope, PNEN usually corresponds to a well-differentiated neuroendocrine neoplasms G1 or well-differentiated neuroendocrine neoplasms G2. Organoid structures in the form of solid nests, trabecular or labyrinthine systems, or structures resembling glands and rosettes, are characteristic. They are accompanied by a varying quantity of tumour stroma and numerous blood vessels surrounding the tumour nests. It is worth emphasising that amyloid deposits are typical for a functioning tumour such as insulinoma, whereas glandular-like structures and psammomatous bodies are characteristic of somatostatinoma. The characteristics of neuroendocrine neoplasm cells are well known to differ from other neoplasms. They are small or medium-sized, with acidophilic or amphophilic and granular cytoplasm. The nuclei are round or oval, usually situated centrally in the cell. A typical feature of a NEN, which helps to distinguish it from adenocarcinoma, is fine-grained chromatin, referred to as ‘salt and pepper’. Apart from the above typical features of neuroendocrine tumours, their cells may present a different picture, creating oncocytic, clear cell, fat-rich and rhabdo-like variants. PNENs may then resemble melanoma, clear cell renal cell carcinoma or adrenal cortical carcinomas. Diagnostic errors are caused by incorrect differentiation between a PNEN and a pancreatic ductal adenocarcinoma or acinocellular carcinoma, or clear cell carcinoma metastases from other organs.

To sum up, pathomorphological diagnostics of pancreatic NENs requires experience on the part of the pathomorphologist, co-operation of an interdisciplinary team of specialists, and access to an immunohistochemical laboratory.

 

Minimal consensus on pathology

Minimal histopathological report for a PNEN should include:

histological type of the neoplasm, considering the division into well-differentiated neuroendocrine neoplasms (NEN), neuroendocrine carcinomas (NEC) and mixed neoplasms (MANEC);

histological G grading referring to well-differentiated neoplasms (NEN G1, NEN G2);

pTNM staging according to ENET and AJCC/UICC classifications (it is important to provide affiliation of the classification in each case);

assessment of surgical margins.

Histopathological diagnosis of NEN must be necessarily confirmed by immunohistochemical tests assessing expression of the neuroendocrine markers: synaptophysin and chromogranin A, as well as the Ki-67 proliferative activity using the MIB1 antigen [53] (*evidence level 3).

2.3. Imaging diagnostics

2.3.1. Endoscopic diagnostics

Classical gastrointestinal endoscopy is practically of no importance for the diagnostics of PNENs.

2.3.2. Ultrasonography Transabdominal ultrasonography

The sensitivity of conventional ultrasonography (USG), mostly performed as the first-line examination in the detection of primary tumours, and in assessment of the staging of the disease, is low for small tumours. On average, ultrasonography detects approximately 30% of primary insulinomas and gastrinomas. The sensitivity of this method increases for detection of hepatic metastases, when it amounts to 50–80%. For larger tumours, mostly non-secreting pancreatic tumours and late-diagnosed glucagonoma, the sensitivity of transabdominal USG is higher [54, 55, 56].

2.3.3. Endoscopic ultrasonography

Endoscopic ultrasonography (EUS) enables precise imaging of the pancreas, and it is the most sensitive of the methods presently used in the diagnostics of pancreatic focal lesions (it detects lesions of 1–2 mm in diameter); normal results of EUS practically exclude the presence of a pancreatic tumour [57]. EUS imaging is useful when the CT scan image is inconclusive. Biopsy is recommended to confirm the neoplastic character of the lesion [58].

EUS enables the ability to:

— localise functioning neoplasms (diagnosed on the basis of clinical and/or biochemical symptoms);

— obtainthematerialforhistopathologicalexamination;

— tattoo small focal lesions before the planned surgical treatment;

— perform diagnostic imaging of non-functioning PNENs;

— conduct screening tests in patients with MEN1.

In the case of small insulin-secreting tumours, the EUS sensitivity is up to 94% [59–64]. Tumour location in the tail of the pancreas or the presence of small, slightly hypoechogenic nodules located deep into the pancreatic parenchyma and multifocal nodules can be a limitation for EUS [59, 65]. According to the literature, in the case of tumours in the pancreatic tail, the sensitivity of the examination can decrease to 60%. Diagnostic sensitivity of the examination in pancreatic gastrinoma tumours is nearly 100%, but it decreases in the case of multifocal lesions and those with an extra-pancreatic location; in the case of gastrin tumours located in the duodenum and outside the pancreatic parenchyma, the sensitivity of the test is estimated to be approximately 50% [63, 66].

EUS is also important in the differential diagnostics of PNENs of ambiguous character, and in pre-operative assessment of the neoplasm stage. There are certain specific ultrasonographic characteristics which allow differentiation between pancreatic carcinomas and neoplasms of the neuroendocrine origin, as well as between functioning and non-functioning neoplasms [67]. The usefulness of EUS for the assessment of the stage of lesions has also been confirmed, particularly for the evaluation of vascular invasion [68].

EUS is also used to perform fine-needle biopsy through the stomach wall. It is believed that this route of access, compared to percutaneous biopsy, reduces the danger of the spreading of neoplastic cells. In the study by Voss et al. [69], the diagnostic accuracy of such biopsy for pancreatic carcinoma was 81%, and for PNEN was 46.7%.

Presently, third generation contrast agents are entering ultrasound imaging. These agents are composed of gas microbubbles in a phospholipid shell, characterised by a long half-life in the bloodstream and enhanced, perfusion-dependent greyscale. Currently, studies are conducted on the use of contrast enhanced ultrasound (CEUS) for the differential diagnostics of pancreatic tumours, including PNENs [70]. CEUS detects tumours smaller than 2 cm in diameter with a sensitivity comparable to EUS (95%). With respect to PNENs, the method’s sensitivity is up to 94%, specificity reaches 96%, the positive predictive value — 75%, and the negative predictive value is up to 99%. The image of neuroendocrine neoplasms has a characteristic echo pattern after intravenous administration of the contrast agent: in the arterial phase, echogenicity increases intensively and quickly decreases as the contrast agent washes out in the venous phase [2]. The highest accuracy in the differential diagnosis of pancreatic neoplasms and in detection of small (less than 1 cm) lesions is achieved by combining the EUS technique with intravenous administration of the contrast agent (Contrast-Enhanced Harmonic Endoscopic Ultrasonography, CH-EUS) [71].

In pre-operative diagnostics, it is possible to inject ink into the tumour tissue during EUS, which enables faster intraoperative location of the lesion. Using this method is important, especially in the case of laparoscopic procedures, during which it is impossible to palpate the pancreatic lesions. Apart from that, precise location of the lesion enables the ability to achieve an adequate resection margin and to preserve healthy pancreatic tissue. However, it is worth emphasising that tattooing may cause acute pancreatitis [72, 73].

A special indication for conducting EUS examination is MEN1. The incidence of pancreatic lesions in this group of patients is estimated as 40–80%. Although functioning neoplasms can be early diagnosed due to typical clinical and biochemical symptoms, nonfunctioning neoplasms (ca. 50% of lesions) in most patients are diagnosed late, which determines a poor prognosis. EUS is recommended as the most sensitive and economically justified method of monitoring these patients, as early detection of a pancreatic lesion enables the implementation of radical treatment.

2.3.4. Intraductal ultrasonography

Intraductal ultrasonography (IDUS) may surpass EUS in the detection of PNENs. In this method, a probe of 2 cm in diameter is introduced into the duct of Wirsung through the duodenoscope channel.

2.3.5. Intraoperative ultrasonography

The sensitivity of intraoperative ultrasound (IOUS) in the detection of small PNENs is similar to that of EUS. The sensitivity of this examination, combined with intraoperative palpation assessment, is up to 97%. In the case of gastrinoma, the sensitivity of the test within the pancreas is close to 100%, but decreases to 58% with extra-pancreatic location. IOUS also allows detection of multifocal tumours and metastases in the liver. IOUS examination is also performed during laparoscopy [74].

2.3.6. Computed tomography (CT) and magnetic resonance imaging (MR)

Presently, according to current guidelines, a spiral multidetector CT (MDCT) and MR imaging are used for the diagnosis of parenchymal abdominal organs. These methods are especially important in the assessment of the stage of the neoplastic disease, and in monitoring of the response to treatment [75]. They are also useful for the assessment of anatomical location and resectability of the primary lesion. Computed tomography enables performing a targeted biopsy from the lesion. The sensitivity of each imaging method depends on the location and type of tumour [64, 76].

Prior to administration of the contrast agent, functioning PNENs are usually isodense, rarely hypodense compared to the remaining pancreatic parenchyma, and calcifications are clearly visible. Most tumours are richly vascularised (insulinoma 80%), so in the arterial phase, MDCT is intensively enhanced. Metastases demonstrate a similar behaviour. Therefore, the MDCT examination should cover both pancreas and liver in the arterial phase. In this phase of the test, it is also possible to assess the tumour/coeliac arteries relation. In the parenchymal phase, the assessment is limited to the pancreas, and it concerns tumour morphology and the level of contrast washing out. The portal venous phase again comprises the pancreas, liver and hepatic portal system [77].

Some researchers have suggested conducting the examination also in the delayed phase (150 seconds after the administration of the contrast agent), and in order to further assess the level of washing out of the contrast material from the tumour. In typical neuroendocrine neoplasm, the contrast enhancement should decrease in the delayed phase relative to the arterial phase by at least 60 HU. Other types of enhancement in PNEN include uneven washout of the contrast agent (from more than half or from less than half of the tumour mass), or slowly increasing enhancement when the tumour is better visible in the equilibrium phase, in which the uptake in the normal pancreatic parenchyma decreases. This is a behaviour characteristic for tumours with a high connective tissue content. In the parenchymal and secretive phase, neuroendocrine neoplasms are not always isodense and are therefore invisible in the CT scan. Certain neoplasms in these phases of examination maintain the enhancement or only begin the process of collecting the contrast agent. Slightly enhanced neoplasms are usually poorly differentiated, so the level of enhancement correlates with the length of patient survival [78, 79]. Non-functioning neoplasm demonstrate a lower enhancement after the administration of the contrast agent, and are heterogeneous due to the necrotic areas. Calcifications in adenocarcinomas are very rare, whereas in functioning and non-functioning PNENs they are found in at least 25% of cases. In larger tumours, the pancreatic duct is dilated, and parenchymal atrophy is observed. The only features that distinguish malignant lesions from benign ones are invasion of the adjacent structures and distant metastases. Hepatic metastases are detected in the arterial phase of the examination [76].

Due to shortened time of scanning, a reduced number of movement artifacts, and obtaining thin (1–2 mm) tissue layers, MDCT enables multi-dimensional and spatial reconstructions, which facilitates imaging of structures smaller than 1 cm, and allows a complete assessment of the vascular invasion of the tumour [80]. The sensitivity of contrast-enhanced MDCT using 1 mm layers in diagnosis of insulinoma reaches 85–94% [81, 82], whereas for various types of NENs the sensitivity of multidetector CT is 50–90%, and the specificity is 96% [2, 83, 84].

The role of CT scans in the assessment of PNEN consists in the description of tumour morphology with precise location, and, with reference to organ-transgressing infiltration, in determination of the adjacent fat tissue invasion, infiltration of the duodenum, common bile duct, stomach, spleen, intestinal loops, adrenal glands, as well as determination of arterial and venous invasion, providing information about the invaded part and length of the vessel. The description should also contain information concerning enlarged regional lymph nodes and the assessment of the liver for metastases. Assessment according to TNM classification should be possible on the basis of CT description [1].

MR conducted according to the optimal protocol has a similar sensitivity in the diagnosis of PNEN as CT, which is up to 80–90%. MR offers a higher tissue resolution in combination with multi-dimensional imaging. Limitations of this method include: reduced availability (compared to CT scanning), higher price, longer duration of examination and the necessity of co-operation with the patient. The method is recommended especially for younger patients, as it does not require the use of ionising radiation, and also in patients whose CT scan image is inconclusive. Neuroendocrine neoplasms are hypointense on T1-weighted images, and hyperintese on T2-weighted images. Intravenous administration of the contrast agent increases sensitivity of the method [77]. In a multi-phase examination following the intravenous administration of the contrast agent, the images are enhanced according to the CT enhancement pattern provided above. In addition, in MR spectroscopy, which uses the chemical shift displacement, it is possible to determine the chemical composition of tissues. A relatively increased lipid content in NENs facilitates differentiation in uncertain cases.

In recent years, a diffusion weighted imaging (DWI) method has also been used, in which the level of water diffusion limitation in the tissue is assessed. Neuroendocrine neoplasms, particularly those with a high connective tissue content, cause limitation of the diffusion of water molecules, which generates an intensive signal in the DWI sequence, accompanied by lowered ADC. DWI is particularly valuable in tumours with a significant connective tissue component, which are poorly or atypically enhanced after intravenous administration of the contrast agent [54, 85].

2.4. Radioisotope diagnostics

The recently observed development of diagnostic methods with the use of labelled somatostatin analogues in examinations using the technique of single proton emission computed tomography (SPECT/CT) and positron emission tomography (PET/CT), also in combination with intraoperative detection with the use of isotope probe, contributes to higher detection rates of PNENs and their metastases. These tests can identify lesions undetected by anatomical imaging methods, increasing the chances of locating the primary focus and determining the actual stage of the neoplasm [76, 86]. They may also be the first-line method in the diagnostics of early recurrence, in monitoring the disease and in choosing the optimal treatment. A positive result of receptor scintigraphy is also the basis for introducing therapy with ‘cold’ and/or ‘hot’ (bound to a radioactive isotope) somatostatin analogues (SSA) [87, 88].

111In-Octreoscan, used until recently in scintigraphic diagnostics, has been substituted in Poland with 99TcEDDA/HYNIC-Tyr(3)-octreotide. The sensitivity of somatostatin receptor scintigraphy (SRS) for tests with the use of 111In-Octreoscan in the diagnosis of the primary lesion for various PNENs has been estimated at 70–100% for gastrinoma, VIPoma and glucagonoma [89], at 50–60% for insulinoma [86], and at ca. 90% for non-functioning tumours. Scintigraphy with the use of 111In-Octreoscan enables the ability to detect approximately 90% of GEP NENs hepatic metastases [90]. Generally, the sensitivity of SRS is estimated to be 71–96% [91], whereas the specificity ranges between 76% and 95% [89, 91]. Using the SPECT technique significantly improves the sensitivity of the method [89]. The usefulness of increasingly popular somatostatin analogues labelled with technet 99m for the diagnostics of PNENs has been confirmed by many authors [87, 92]. 99mTc-EDDA/HYNIC-Tyr(3)-octreotide (99mTc-EDDA/HYNIC-TOC) 99mTc-[99mTc-EDDA/HYNIC] octreotate demonstrates a higher uptake by the pancreatic and pituitary tumour cells having somatostatin receptors than octreotide. The possibility of combining scintigraphic and tomographic images with the use of fusion image SPECT/CT enables a precise detection of the anatomical location of the lesion visible in molecular imaging, and contributes to increased diagnostic sensitivity and specificity [93].

Presently, the most sensitive examination in the diagnostics of well-differentiated NENs is definitely the PET/CT test with the use of somatostatin analogues (SSA) labelled with 68Ga: DOTA-TOC, DOTA-TATE and DOTA-NOC, and in the future probably also with 64Cu-TETA-octreotide [94]. In the diagnostics of PNENs, it is also possible to use the PET/CT test with the use of 18F-FDOPA. The usefulness of 18F-FDOPA has been evaluated in different types of NENs and at different stages of the neoplastic process. Becherer et al. demonstrated a higher diagnostic sensitivity of 18F-FDOPA PET compared to SRS and CT scanning in patients with advanced NENs, both with regard to staging and in the diagnostics of osseous metastases. However, SRS proved superior in planning treatment with SSA [95]. The examination with 18F-FDOPA enables the exclusion of artifacts related to physiological activity in the peripancreatic tissues [44]. Other studies comparing 68Ga with 18F-FDOPA have indicated the indisputable superiority of the 68Ga-DOTA-TATE test in the detection and staging assessment of NENs, whereas PET/CT examination with 18F-FDOPA should be performed when the test with gallium is negative [96]. In the case of insulinoma, the diagnostic value of 18F-FDOPA is disputable [97]. Another tracer used in diagnostics of PNENs is 11C-5-hydroxytryptophan (5-HTP) [2]. It is not used in Poland. 18F-Fluorodeoxyglucose (FDG) is used in the diagnostics of fast-growing and aggressive PNENs and PNECs with a poor prognosis.

The next step to improve sensitivity of location diagnostics of small PNETs (gastrinoma, insulinoma) is using an intraoperative radioisotope probe (RGS) [98, 99].

In recent years, new diagnostic tracers for PNENs have been introduced. They enable the location of certain types of functioning tumours, and due to diagnostic effectiveness in the future they may become important imaging tools for PNENs. One of them is a labelled analogue of glucagon-like peptide-1 (GLP-1). Due to a very high expression of receptors for GLP-1 in some neoplasms (in 100% of benign insulin-secreting tumours), scintigraphy with the use of labelled GLP-1 analogues may become a diagnostic method competing with SRS [100]. Several reports concerning imaging of insulinoma on animal models and in humans have been published. A high tracer uptake by this tumour was demonstrated (high tumour/background ratio), and the quality of the received image was assessed as very good. Using a GLP-1 analogue labelled with 111In, insulinoma implanted in a mouse was completely destroyed. In the study, the GLP-1 analogues labelled with 111In (e.g. [Lys40(Ahx-DOTA-111In)NH2]-Exendin-4), 99mTc ([Lys40(Ahx-HYNIC-99mTc/EDDA)NH2]-exendin-4) and 68Ga were used. Preliminary study results indicate a lack of receptor expression for GLP-1 in most malignant forms of insulinoma (positive SRS is more common in these cases), which suggests the usefulness of imaging with labelled GLP-1 analogues for the differentiation of benign and malignant insulin-secreting tumour forms [101, 102].

2.5. Location diagnostics of different PNENs

2.5.1. Insulinoma

Most frequently they are small tumours, less than 2 cm in diameter (60–70% of cases), usually classified as group 1 according to the WHO classification; they are mostly single (85%) and located in 99% of cases in the pancreas, with a similar prevalence for all parts of the organ [10]. While conducting location diagnostics in the search for the cause of hypoglycaemia with hyperinsulinism, it should be noted that in approximately 4% of cases, the reason is hyperplasia of β cells (nesidioblastosis; NIPHS, noninsulinoma pancreatogenous hypoglycaemia). In the case of insulinoma, the most sensitive imaging examinations include endoscopic ultrasonography (EUS) and intraoperative USG. The usefulness of classical USG, EUS, IOUS, CT and MRI is discussed in detail in the section concerning the imaging diagnostics of PNENs.

Another test used in the diagnostics of insulinoma is SRS. It is important to note that only 50-60% of insulinoma tumours demonstrate somatostatin receptor expression (according to the literature data, the frequencies of expression for different SSTR types in insulinoma are as follows: SSTR1 — 51%, SSTR2 — 69%, SSTR3 — 62%, SSTR4 — 39%, and SSTR5 — 66%) [86]. If the results of other imaging tests are negative, a PET/CT scan with 68Ga-somatostatin analogue may be performed [4]. Transhepatic portal venous insulin sampling (THPVS) and angiography with a selective calcium stimulation test [103] are very rarely used in diagnosing insulinoma. The methods are practically used only when other imaging techniques do not enable the locating of the focal lesion [10]. In the near future, GLP-1 analogue will probably play an important role in the diagnostics of hardly detectable, small insulinomas, due to GLP-1 receptor expression in all tumours of this type.

2.5.2. Gastrinoma

Gastrinoma is found mostly within the triangle: pancreatic head — duodenum — hepatic hilum. In 48–60% of cases, lymph nodes and hepatic metastases are present at the diagnosis, but in some groups of patients, the proportion of malignant neoplasms is up to 90% [104]. Multifocal lesions are also possible. The usefulness of USG, EUS, intraoperative USG, IOUS, CT and MRI examinations is presented in the section concerning the imaging diagnostics of PNENs.

Other examinations used for the diagnostics of gastrinoma include:

— somatostatin receptor scintigraphy (SRS). According to different authors, the sensitivity of gastrinoma detection ranges between 50% and 100% (according to the literature data, the frequencies of expression for different somatostatin receptors (SSTR) types are as follows: SSTR1 — 71%, SSTR2 — 50%, SSTR3 — 92%, SSTR4 — 78%, SSTR5 — 81%) [105]. SRS is the best examination to assess the early stages of the disease and the presence of distant metastases, but sensitivity of the test decreases to 50% if the tumour is smaller than 1 cm [4];

— intraoperative radioisotope probe. This method improves the sensitivity of detection of pancreatic primary lesions and metastases to the surrounding lymph nodes and to the liver;

— in the diagnostics of gastrinoma, scintigraphy with the GLP-1 analogue may also be used, due to the GLP-1 receptor expression in this tumour.

In the location diagnostics of small tumours, the combined use of a few diagnostic methods seems reasonable. In certain cases also performing angiography (the sensitivity of angiography is estimated at 30-50%) with venous catheterisation (AVSV) may be considered. In the case of gastrinoma located in the duodenum, intraoperative transluminescence is also used.

2.5.3. Location diagnostics of glucagonoma, VIPoma, somatostatinoma, non-functioning tumours and ACTHoma

At the moment of diagnosis, glucagonoma, somatostatinoma and NF-PNETs are usually large (approximately 5–6 cm), whereas VIPoma is slightly smaller (ca. 2 cm). The lesions are usually diagnosed late, and in approximately 70–90% of cases metastases are found already at the diagnosis [16, 105]. Due to the size of lesions, they are easier to find by means of classical imaging methods (USG, CT, MRI). SRS, whose diagnostic sensitivity is 70–100%, is a standard examination in the assessment of the primary lesion, clinical staging (detection of metastases to the liver, lymph nodes, adrenal glands, spine), and in qualification for receptor radiotherapy [16, 86]. SSTR1 and SSTR2 expression is observed mostly in glucagonoma, SSTR5 in somatostatinoma, SSTR2 in VIPoma, and SSTR1, SSTR2, SSTR3 and SSTR5 in nonfunctioning neoplasms. Rare ACTHoma neoplasms also demonstrate somatostatin receptor expression. In the case of RF-PNENs, EUS is not recommended as the first-line procedure, but it may be used when MDCT, MRI and SRS-SPECT results are inconclusive. EUS may be useful in pre-operative diagnostics, whereas it is rarely necessary in patients with hepatic metastases [4].

2.5.4. Pancreatic endocrine carcinomas (PNECs)

In the location diagnostics of poorly differentiated PNECs and their metastases, all imaging examinations may be used: USG, CT, MRI, 18F-FDG PET, as well as SRS in tumours with overexpression of somatostatin receptors.

Summary of the diagnostics of PNENs

In the diagnostics of PNENs, both classical imaging techniques and nuclear medicine tools are used. The basic examination for patients with a PNEN is EUS, which enables location of the primary tumour site. Another examination is the SRS with 68Ga or 99Tc-DOTA TOC, which enables location of the primary lesion, but also assessment of the stage of the disease and qualification for treatment with ‘hot’ SSA. CT or MRI are next in the diagnostics of PNENs; their main role consists in the assessment of the stage of the disease. A PET/CT scan with 18FDOPA may be an alternative diagnostic method if SRS results are negative. In the diagnostics of insulinoma, the role of a new isotope-labelled GLP-1 analogue is widely discussed.

 

Minimal consensus on imaging

The main examinations recommended in the diagnostics of PNENs include: EUS, CT, and MR, and next a radioisotope examination with a labelled somatostatin analogue (68Ga or 99Tc-DOTA TOC PET/CT,SPECT/CT) (*evidence level 3).

3. Treatment of PNENs

3.1. Surgical treatment

Surgical treatment is the therapy of choice in the case of PNENs, as it is associated with significantly prolonged patient survival [2]. The development of diagnostic methods has improved the detection of small, asymptomatic tumours. Most non-functional neoplasms of ≤ 2 cm in diameter are benign and demonstrate a moderate risk of becoming malignant. Only 6% of non-functional, accidentally diagnosed PNENs present histopathological characteristics of malignancy [2]. In certain cases, tumours of ≤ 2 cm in diameter diagnosed accidentally may be observed for the first year, performing tests at three-month intervals, then every six months for the next three years [2]. Due to the lack of clear recommendations, the decision on the course of treatment should be taken by a multidisciplinary team of doctors experienced in the management of PNENs (*evidence level 4). When choosing surgical treatment, it is necessary to consider short-term and long-term effects of this therapy. According to the WHO classification, there is a correlation between the tumour size and its potential malignancy. Tumours of > 2 cm require an extensive surgery (*evidence level 3) [44].

In multiple endocrine neoplasia type 1 (MEN1), if multiple lesions occur, it is recommended to remove them preventively before they become malignant; however, this approach in the case of small, non-functional neoplasms is still controversial (*evidence level 3) [2]. In all cases, an intraoperative USG examination is recommended. The presence of multiple tumours sometimes requires a whole-organ resection. It is known that nonfunctional neoplasms associated with MEN1 should be removed if they are larger than 2 cm in diameter, fast-growing (annual growth of > 0.5 cm), and if metastases occur [2].

The type of surgical treatment of PNEN depends on its size, location, invasion of the adjacent organs, presence of distant metastases and the level of tumour malignancy, patient’s general condition and the ability to control the clinical symptoms (*evidence level 4). Patients are qualified for a radical or palliative treatment, which only improves the quality of life (*evidence level 4). In the case of tumours located in the head of the pancreas, pancreatoduodenectomy is performed, whereas in tumours located in the body or tail of the pancreas, distal resection is conducted, with or without splenectomy (*evidence level 4). In certain cases of small and well-demarcated PNENs (non-functional neoplasms and insulinomas < 2 cm), atypical resections may be performed, including enucleation and resection of the middle segment (*evidence level 3) [106]. Resection of the middle segment is performed only in the case of small lesions located in the pancreatic body. The condition of tumour enucleation is continuity of the duct of Wirsung [2]. Resection is necessary if the tumour is located < 3 mm from the pancreatic duct [4]. Enucleation of the lesion entails the risk of damaging or closing the duct of Wirsung, which is associated with complications [107]. These include acute postoperative pancreatitis and pancreatic fistula. Apart from the above complications, resection of a large part of the pancreas may cause the symptoms of exocrine and endocrine pancreatic insufficiency [108]. In certain cases, it is possible to conduct central pancreatectomy with a Roux-en-Y anastomosis of the pancreatic tail with a loop of the small intestine, and closing off the body of the pancreas.

Tumour resection should be considered even in the presence of metastases, including hepatic metastases, if they are potentially resectable, and the patient meets the criteria for the surgery (*evidence level 4) [1, 44]. As PNENs are often malignant, it is necessary to remove the regional lymph nodes during resection (*evidence level 3) [1, 108, 109]. In the case of enucleation and resection of the middle segment, it is also recommended to remove lymph nodes for histopathological examination [2, 107]. It is generally believed that PNECs should not be operated if disseminated metastases have been found in the diagnostic process (*evidence level 3) [1].

Surgical treatment intended to remove the tumour (in the case of a resectable tumour) or reduce its mass (palliative therapy) in patients with disease limited to the primary tumour and regional lymph nodes should be standard procedure (*evidence level 4) [44]. Resection should be performed only in those centres specialising in surgery of the pancreas. Laparoscopic resection of the pancreas is increasingly common, but the decision concerning whether to use an ‘open’ or a laparoscopic method should be taken by a pancreatic surgery specialist in the referential centre (*evidence level 3). Distal resections and laparoscopic enucleation of pancreatic tumours are presently considered to be safe [2]. In the case of a PNEN, an intraoperative USG examination is recommended.

The most common functional neoplasms are insulinoma and gastrinoma, while other tumours are rare RF-PNENs [4]. Gastrinoma is most often located in the head of the pancreas; in 60–90% it is a malignant neoplasm, and due to a frequent invasion of the lymph nodes, there are indications for regional lymphadenectomy [14]. It is recommended to remove the lesion radically to prevent hepatic metastases, which considerably worsen the prognosis. The scope of procedures depends on the tumour location and size, and comprises enucleations, resections of the middle segment, distal resections and pancreatoduodenectomies [109]. Laparoscopic procedures are not recommended [4]. In ZollingerEllison syndrome (ZES), which may be associated with MEN1 syndrome, surgical treatment is indicated if the tumour is larger than 2 cm. This approach is intended to prevent metastases [44] (*evidence level 3). Pancreatoduodenectomy is the recommended procedure (for tumours located in the head of the pancreas), as less extensive procedures are associated with recurrence in 90% of cases [4, 14].

Insulinomas are in 90% of cases benign; their removal does not require regional lymphadenectomy and where there is precise preoperative localisation of the tumour, laparoscopy is effective [110]. In the case of a suspected malignant insulinoma, or recurrence of the tumour, radical treatment is recommended, including excision of the recurrence and the possible hepatic metastases [4]. RF-PNEN include: VIPoma, glucagonoma, somatostatinoma, GRHoma and ACTHoma. In this group of tumours, radical surgical treatment is recommended even if hepatic metastases occur, and the scope of resection and lymphadenectomy corresponds to the procedures implemented in gastrinoma. Laparoscopic procedures are not recommended (*evidence level 3) [1, 4].

In advanced F-PNENs, resection is intended to reduce the symptoms and the tumour mass. Cytoreduction may be considered if removal of 90% of the tumour mass is possible, even if hepatic metastases are present. Removal of 90% of the visible tumour mass is possible in only 5–15% of cases [14, 111]. R1 resections of PNENs are not associated with a worse survival rate than R0 resection [107]. Cytoreduction may be performed with radiofrequency thermoablation (RFA) (*evidence level 3), which can also be conducted laparoscopically. This method may be used if there are fewer than ten focal lesions in the liver, and if the largest one is < 5 cm in diameter (optimally 3 cm). This method enables the control of symptoms in over 90% of patients [112].

Radical excision of hepatic metastases is the ‘gold standard’ in the therapy of advanced PNENs, therefore resection should be performed whenever it is possible (*evidence level 4). The method of resection depends on the patient’s general condition, and the size, location and number of metastases. It comprises enucleation, wedge excision of a fragment of the liver, excision of a segment/segments, non-anatomical resection or hemihepatectomy. Resection of hepatic metastases of PNENs is considered only in cases of G1 and G2 neoplasms [113]. It depends on the resectability of the lymph nodes, lack of micronodular or non-resectable dissemination in the peritoneum, or distant metastases outside of the abdominal cavity [113]. R2 resection should be considered in selected cases of functional neoplasms which do not respond to conservative treatment, in order to reduce the intensity of the symptoms (*evidence level 3). Excision of the primary tumour, lymph nodes and hepatic metastases, combined with thermoablation, may reduce the tumour mass by more than 90% [113]. In the case of unresectable metastatic lesions in the liver, cholecystectomy should be performed during surgery to prevent ischaemic complications of the gall bladder resulting from a possible implementation of (chemo) embolisation. Resection of hepatic lesions may be performed in one or two stages, depending on the location and size of the metastases [107]. Other methods of treating metastases include locoregional therapies (variants of ablation, embolisation) and liver transplantation. It is assumed that transplantation is conducted in selected groups of patients with exacerbated symptoms associated with hormonal secretion. Patients who may benefit from transplantation are those under the age of 50, without metastases outside the liver, and with a low expression of Ki-67 and E-cadherin [109].

If resection of hepatic metastases is impossible (unresectable or inoperable lesions), the recommended treatment methods include hepatic artery embolisation (HAE), transarterial chemoembolisation (TACE) or embolisation with the use of isotope (*evidence level 3). Currently, these methods are considered to be safe [14]. RFA, cryoablation and microwave ablation (MWA) can be used for tumours ≤ 5 cm [111].

In the case of a diagnosis of peritoneal dissemination, surgical treatment is controversial and is recommended only in a selected group of patients. Even in some patients with unresectable liver disease, resection of the primary tumour together with macroscopic intraperitoneal nodules makes it possible to focus further therapies exclusively on the liver disease [114]. There is no consensus on the simultaneous resection of hepatic and intraperitoneal lesions. If an extensive surgery of the liver with resection of peritoneal lesions is necessary, dividing the procedure into stages, conducting the resection in a specialist centre, and introducing multidirectional treatment should be considered. Presently, the combination of surgery and perioperative intraperitoneal chemotherapy, as well as intraoperative hyperthermic intraperitoneal chemotherapy (HIPEC) are in the experimental phase [114].

If tumour resection and the elimination of symptoms are not possible, a palliative surgical management is implemented, which can significantly affect the quality of life. This is used after exhausting all non-surgical methods, mainly when the tumour is responsible for mechanical jaundice, chronic pain and gastrointestinal obstruction or bleeding. The treatment method is individualised for each patient. If mechanical jaundice occurs, it is recommended to perform anastomosis between the bile duct and intestine, or drainage of the bile tract. When an unresectable pancreatic tumour disturbs the passage of food through the duodenum, bypass surgery is recommended, usually a gastrointestinal anastomosis. The method of surgical management of chronic pain is coeliac plexus neurolysis and/or thoracoscopic section of the splanchnic nerve. Treatment of patients with PNEN should be comprehensive and conducted by a multidisciplinary team of doctors; the surgery should be performed in a centre specialising in pancreatic surgery (*evidence level 3) [44].

 

Minimal consensus on surgical treatment

Accidentally detected non-functional neoplasms of 2 cm in diameter, without evidence of histopathological malignancy may be observed, and the decision on the course of treatment should be taken by a multidisciplinary team of doctors experienced in the management of PNENs. Tumours of > 2 cm require an extensive surgery with lymphadenectomy.

In certain cases of small (< 2 cm) and well-demarcated PNENs (non-functioning neoplasms and insulinomas), atypical resections may be performed, including enucleation and resection of the middle segment (it is necessary to collect the lymph nodes for histopathological examination). Distal resections and enucleations may be performed laparoscopically.

Tumour resection should be considered even in the presence of metastases, including hepatic metastases, if they are potentially resectable and the patient meets the criteria for the surgery. It is believed that PNECs should not be operated if disseminated metastases have been found in the diagnostic process.

In advanced functioning PNENs, resection is intended to reduce the symptoms and the tumour mass. Cytoreduction may be considered when the removal of 90% of the tumour mass is possible, even if hepatic metastases are present (*evidence level 3).

In cases of unresectable hepatic metastases, the recommended palliative treatments include HAE, TACE or embolisation with the use of a radioisotope. RFA, cryoablation and MWA can be used in tumours 5 cm (*evidence level 3). Liver transplantation is conducted in a selected groups of patients with exacerbated symptoms associated with hormonal secretion. Patients who may benefit from the transplant are those under the age of < 50 years, without metastases outside the liver, and with a low expression of Ki-67 and E-cadherin (*evidence level 3).

3.2. Endoscopic treatment of PNENs

The treatment of PNENs is generally surgical, and endoscopic management is only symptomatic.

Endoscopy may be used in the symptomatic treatment of:

— mechanical jaundice (prosthesis of the biliary duct);

— obstruction of the gastrointestinal tract (prosthesis of the gastrointestinal tract lumen);

— control of gastrointestinal bleeding (the use of endoscopic hemostatic methods).

— EUS-controlled coeliac plexus neurolysis (CPN), described for the first time in 1996, involving administration of 0.25% bupivacaine solution, followed by 98% alcohol, is an alternative method for the management of chronic pain associated with pancreatic tumours [115].

In recent years, single cases of using EUS for ablation of F-PNETs secreting insulin have been reported [116–118]. It is possible that in the future endoscopic EUS-controlled ablation of PNETs, involving administration of cytotoxic agents, alcohol or using thermoablation will become a therapeutic method for patients who cannot be treated surgically (*evidence level 4).

3.3. Medical treatment

The main purpose of pharmacological treatment is prevention of the symptoms of the disease and maintaining the patient’s good quality of life (QoL) for the longest possible time [44]. Prior to planning the treatment, the tumour size, presence of metastases, histological grading and the profile of secreted peptides and markers should be determined (*evidence level 4).

The choice of the treatment method depends on the symptoms, staging of the disease, the level of radiotracer uptake in receptor scintigraphy and histological characteristics of the tumour [119] (*evidence level 4).

For non-surgical NENs, the goal of the treatment is alleviation of symptoms of the disease, maintaining optimal QoL and, if possible, prolonged survival (*evidence level 5).

3.3.1. Symptomatic treatment

Symptomatic treatment should be started when the clinical and biochemical symptoms indicate hormonal activity of the NEN, even before the precise location of the primary site or confirmation of metastases. The symptoms associated with excessive secretion of hormones by PNENs may impair the patient’s quality of life, and in certain cases they may be life-threatening (e.g. severe diarrhoea and hypokalaemia in VIPoma or carcinoid crisis).

Pharmacological treatment mostly involves somatostatin analogues and other medications, such as IPP in gastrinoma or diazoxide in the case of insulinoma. Additional symptomatic medications, such as loperamide, cholestyramine and corticosteroids are used if necessary. Bisphosphonates can be used for pain management in patients with osseous metastases.

 

Functioning pancreatic neuroendocrine neoplasms (F-PNENs)

Somatostatin analogues (SSA)

Using somatostatin analogues (octreotide, lanreotide) is the ‘gold standard’ therapy of functioning PNENs, regardless of the tumour size [1, 120]. The recently published CLARINET study, involving 204 patients with GEP NENs (45% of patients with PNENs), also proved the antiproliferative effect of Lanreotide Autogel in a randomised, placebo-controlled phase III trial [121] (*evidence level 1).

New somatostatin analogues

SOM-230 (pasireotide) is a new analogue of somatostatin (SSA). In phase II studies, pasireotide was used for symptomatic treatment in patients resistant to conventional therapy (octreotide and lanreotide).

Interferon α

Interferon α is used in the treatment of both functioning and non-functioning NENs, in monotherapy or combined with long-acting SSA, if the patient does not respond to the treatment with maximum SSA doses. Interferon a may also be used in symptomatic treatment, but it is usually introduced as a second-line treatment due to its unfavourable toxicity profile [1]. Sometimes it is used as an adjuvant therapy in patients with clinical syndromes which cannot be controlled with SSA.

Insulinoma

Pharmacological treatment of insulinoma is intended to prepare patients for a surgical procedure, or to achieve biochemical control in patients with inoperable metastatic insulinoma. Apart from frequent meals of small volume, patients require intravenous glucose administration. Despite this treatment, hypoglycaemia often requires additional medications to control the serum glucose concentration. In most patients with insulinoma, diaxozide has proved to be effective in controlling the symptoms of hypoglycaemia [4]. Diazoxide is used for short-term treatment of patients with insulinoma awaiting surgery, or for long-term treatment of patients with inoperable tumours. Diazoxide is an antihypertensive medication with an additional hyperglycaemic effect, as it inhibits insulin secretion by a direct action on pancreatic β cells and activation of glycogenolysis. The recommended daily dose is 50–300 mg orally, up to 600 mg/d. This is usually an effective treatment in controlling the symptoms of hypoglycaemia in patients with insulinoma. Adverse events, including oedema, increased body weight, hirsutism and renal function disorders are common, but usually tolerable.

Diazoxide therapy is often supported with hydrochlorothiazide at a dose of 25 mg/day, which prevents oedema, hyperkalaemia and increases the hyperglycaemic effect of diazoxide.

Verapamil and diphenylhydantoin (phenytoin) can be used to control glycaemia, as an alternative to diazoxide, in some patients with insulinoma. Corticosteroids, including prednisolone, are usually used in insulinoma patients resistant to the treatment of hypoglycaemia.

SSA (octreotide and lanreotide) are used in patients with confirmed somatostatin receptor type 2 expression on the tumour cells [119]. They are often ineffective in controlling hypoglycaemia (50–60% of insulinomas) and their effect on the blood glucose concentration varies [122]. In some cases, they may even intensify hypoglycaemia by inhibiting glucagon secretion [4, 123].

In some patients, using interferon a may be beneficial in treating hypoglycaemia [34]. An mTOR inhibitor — everolimus — is one of the medicines controlling insulin secretion and hypoglycaemia in patients with malignant insulinoma [4].

Gastrinoma

Pharmacological treatment of gastrinoma is discussed in the section on gastroduodenal NENs (pp. 444–458).

VIPoma (symptoms: watery diarrhoea, hypokalaemia, achlorhydria (WDHA), Verner-Morrison syndrome)

Hydration and supplementation of electrolytes is recommended, as they may considerably improve the patient’s clinical condition. In patients with VIPoma, accompanied by a rare life-threatening syndrome, administration of SSA significantly relieves the symptoms (in 80–90% of patients) and lowers the concentration of vasoactive intestinal peptide (60–80%) [124]. Biochemical improvement does not always correlate with clinical improvement, so a dose titration based on patient’s clinical condition is necessary. Corticosteroids are used in patients with life-threatening diarrhoea which does not respond to the maximum doses of SSA.

Glucagonoma

Following introduction of SSA treatment, 80–90% of patients with glucagonoma demonstrate a visible clinical improvement (reduced skin lesions due to necrolytic erythema), although the treatment is less effective in controlling diabetes and weight loss. SSA reduces blood glucagon concentration in approximately 60% of patients, although normalisation of this parameter is unlikely [125].

Zinc salts may be used in patients with glucagonoma to prevent further skin damage. Antithrombotic prophylaxis should be considered in all patients with NEN associated with an increased risk of thromboembolic complications (e.g. glucagonoma).

Long-acting SSA are also effective in fighting the symptoms of ectopic hypersecretion in some cases of somatostatinoma [4]. They may also be useful in patients with paraneoplastic syndromes, e.g. Cushing’s syndrome and acromegaly, associated with ectopic secretion of ACTH or the growth-hormone-releasing hormone (GHRH).

In recent years, there has been growing interest in the use of glucocorticosteroid receptor antagonists, e.g. mifepristone, as well as dopamine agonists, e.g. cabergoline, in the treatment of Cushing’s syndrome.

SSA have been proven effective in controlling hypercalcaemia associated with the hypersecretion of the parathyroid hormone-related peptide (PTHrP) in rare PNENs secreting PTHrH [126].

Non-functioning pancreatic neuroendocrine neoplasms (NF-PNENs)

Somatostatin analogues demonstrate antiproliferative effects, confirmed in patients with NENs. PROMID was the first phase III study, which revealed such effects of octreotide in the midgut tumours, both functioning and non-functioning ones [127]. In patients with PNENs, observational studies have demonstrated partial or complete response in less than 10% of patients, and radiological stabilisation of the neoplasm in 24–57% of patients [128–130]. The results of the CLARINET phase III study have confirmed the antiproliferative effect of Lanreotide Autogel in patients with nonfunctioning PNETs with low proliferation index (Ki-67 < 10%) [121, 131].

3.3.2. Chemotherapy and targeted treatment Chemotherapy is discussed in detail in section I of "Diagnostic and therapeutic guidelines for gastroentero-pancreatic neuroendocrine neoplasms (recommended by the Polish Network of Neuroendocrine Tumors)" (pp. 418–443)

Given the present state of knowledge, there is no evidence that any adjuvant therapy (i.e. additional therapy after radical surgery) has a positive effect on extending disease-free survival (DFS) and/or overall survival (OS) of patients with PNENs. This applies primarily to G1/G2 NENs [132]. In the case of neuroendocrine carcinoma (NEC), an adjuvant therapy based on platinum analogues should be considered. Although there are no phase III randomised prospective studies, it seems that chemotherapy including cisplatin (or carboplatin) with etoposide may prolong disease-free survival in patients with NECs (*evidence level 4). The use of adjuvant radiotherapy or radiochemotherapy is not scientifically supported.

In patients with advanced NECs, the basic therapy is chemotherapy including platinum analogues plus etoposide [132] (*evidence level 3).

The best results in the chemotherapy of advanced NENs have been achieved in G1/G2 NETs of pancreatic origin. The use of streptozotocin (STZ) in monotherapy resulted in a response rate (RR) of approximately 36%, and OS of ca. 17 months. Combining streptozotocin with 5-fluorouracil (5-FU) increased the response rate to 63% and extended the mean overall survival to 26 months [133]. A breakthrough in chemotherapy of G1/G2 NENs was the phase III study by Moertel et al. in 1992 [134], in which 69 patients were randomised to two chemotherapy arms: streptozotocin-based, i.e. with doxorubicin (DOX) v. chemotherapy with 5-FU, demonstrating RR of 69% v. 45%, respectively, with the mean clinical response time of 18 months v. 14 months, and the mean overall survival of 26 months v. 17 months (*evidence level 3).

The effectiveness of the chemotherapy of well/medium-differentiated GEP NENs should be considered separately for neoplasms of pancreatic origin and those with different locations (stomach, duodenum, small intestine, appendix and colon). Indirect comparisons of the results of clinical studies involving patients with GEP NENs have demonstrated a higher probability of response in patients treated due to PNENs (15–35% compared to 5–15%) [135].

In well-differentiated PNENs, the highest activity in monotherapy (response rate: 20–40%) is demonstrated by streptozotocin, doxorubicin, fluorouracil, dacarbazine and temozolomide. Multi-drug regimens are more effective then monotherapy regarding the effect on the response and survival rates (mean survival — 15–30 months).

The expert panel recommends combining streptozotocin (the medicine is not registered in Poland, available as direct import) with doxorubicin and 5-fluorouracil, and using a two-drug chemotherapy in patients with a greater risk of complications or not qualifying for the treatment with anthracyclines.

3.2.3. New targeted therapies [44]

In patients diagnosed with advanced PNENs (locally advanced stage, without a possibility of surgical treatment and disseminated stage), it is now possible to use molecularly targeted drugs (sunitinib and everolimus) in the case of progression of the disease (progression within the last 12 months assessed on the basis of imaging examinations results and the RECIST classification). Sunitinib is indicated in the case of well-differentiated neoplasms (NENG1), and indications for everolimus comprise welland moderately-differentiated neoplasms (NENG1 and NENG2) [136, 137]. These medications — everolimus (a serine/threonine kinase — mTOR — inhibitor) and sunitinib (a tyrosine-kinase inhibitor) [138] significantly extend progression-free survival (PFS) by approximately 5.5–6 months. Adding everolimus to octreotide (the RADIANT-3 trial) extended progression-free survival by approximately five months (16.4 v. 11.3) compared to monotherapy with SST analogue.

In patients with malignant insulinomas resistant to conventional treatment, using everolimus significantly improved the control of glycaemia [139, 140].

Everolimus and sunitinib may, in justifiable cases, be used as the first-line treatment in patients with advanced well-differentiated PNENs (documented radiological progression within 12 months)(*evidence level 1).

Using sunitinib and everolimus is associated with a number of unresolved problems (e.g. the order of use for various treatment methods, selection of patients for specific medications, the ability to anticipate effectiveness, and adverse reactions). The value of sunitinib and everolimus in poorly differentiated PNECs, and the possibility of combining them with other methods (e.g. adjuvant postsurgical treatment) also require clarification.

The effectiveness of pazopanib (another multikinase inhibitor), demonstrated in the phase II studies, needs to be confirmed, which is particularly important due to a generally better tolerance of the treatment.

 

Minimal consensus on the medical treatment in pancreatic NENs

Functioning G1/G2 PNENs — somatostatin analogues (*evidence level 1), and in the case of progression of the disease, adding everolimus (*evidence level 1).

Advanced non-functioning G1/G2 PNENs — systemic chemotherapy including streptozotocin with doxorubicin (± 5-FU) (*evidence level 3) and/or a somatostatin analogue (*evidence level 1). If the disease progresses after chemotherapy, everolimus or sunitinib (*evidence level 1). Targeted therapy can be considered as first-line therapy in selected cases as an alternative treatment to chemotherapy.

The basic treatment of PNEC is chemotherapy based on the cisplatin plus etoposide regimen (*evidence level 3).

3.4. Radioisotope treatment

Isotope therapy with labelled SSA (PRRT, peptide receptor radionuclide therapy) is now becoming a recognised form of palliative treatment, giving a chance for stabilisation or partial regression of the neoplastic disease, and less often for a complete remission [141, 142]. De Jong et al. [141] in 1998 published the results of a multicentre study involving a group of 256 patients with GEP NENs, including PNENs, treated with 90Y DOTA TOC.

In 27% of the patients, a partial response to the treatment was observed, and in individual cases a total response was achieved. The results of the 90Y/177Lu DOTA TOC therapy in a group of 20 patients, including 15 with PNEN, were presented in 2006. Regarding the patients with PNEN, the authors observed stabilisation of the disease in eight patients, partial remission in four patients, and progression of the disease in three patients [143]. Presently, more common in PRRT is [Tyr3]octreotate (DOTA TATE), an analogue which demonstrates a higher affinity to SSTR2 than the previously used ones. The therapy has been successfully conducted in a few centres in Poland, also in patients with PNENs, after confirming a high somatostatin receptor expression in the primary/meta tumour in SPECT or PET examination. It is now believed that, similarly to GEP NEN of different locations, the therapy may be introduced without a prior chemotherapy. Based on the observations in patients treated with chemotherapy before the isotope treatment, is has been established that myelotoxicity and nephrotoxicity occur more often.

Similarly to other GEP NENs, the main indications for the treatment of PNEN with labelled SSA are advanced and inoperable NENG1 or NENG2. In individual cases, this therapy may be used in NEC with a sufficient somatostatin receptor expression, especially if the disease progresses and other treatment options have been exhausted [2, 4, 34]. Isotope therapy may be considered in the case of non-surgical recurrence and primarily, as a form of neoadjuvant treatment if surgical management is impossible due to a significant local advancement of the tumour [144]. Recent reports point to the possibility of using PRRT much earlier, before the surgery of unresectable lesions, mostly in the case of PNENs. The authors used both 90Y and 177Lu isotope combined with a SSA. Among the patients receiving PRRT as noeoadjuvant treatment of an inoperable primary tumour, there were single cases of patients with hepatic metastases, in whom the isotope therapy resulted not only in regression of the tumour mass, but also of the metastatic lesions [145, 146]. Due to a limited group of patients receiving this form of treatment, there is no strong evidence for introducing PRRT prior to a non-surgical procedure in the management guidelines; however, this form of treatment may be individually considered, depending on the patient’s clinical condition, advancement of the disease and the proliferation index of the neoplasm. Each time while deciding on the isotope treatment, adverse reactions associated with this form of therapy should be taken into account. In the case of PNENs with insufficient SSTR2 expression, indications for the 90Y and 177Lu DOTA TATE therapy may be limited. In overexpression of SSTR5, another radiotracer, i.e. 90Y DOTA LAN may be used [34]. Expression of this type of receptor may also be present in well-differentiated neoplasms, especially those which lost the active SSTR2 during therapy, as a result of treatment with SSA (tachyphylaxis) [34].

PRRT may also be used to treat functioning PNENs. In the case of malignant lesions, it is possible to conduct isotope treatment [10, 34] as a form of palliative management with temporary therapeutic response. In patients with diffuse gastrin tumour and positive receptor scintigraphy results, eligibility for PRRT should be considered. However, similarly to other PNENs, this therapy requires studies involving a larger number of patients in order to establish its actual therapeutic value [104]. Based on individual reports in the literature, it is known that gastrinomas respond to therapy faster, but early progression is relatively frequent [34].

The treatment with ‘hot’ SSA is a relatively new form of therapy, used mainly as palliative treatment, without any expectations regarding the impact on partial remission of the disease or the patient’s survival. Some new publications have indicated a possibility of using PRRT as second-line therapy in the case of disease progression, following stabilisation or remission achieved with this method. It appears that re-implementation of isotope treatment at the maintained expression of somatostatin receptors may prolong the patient’s survival without a significant exacerbation of adverse reactions associated with this therapy [147]. Radioisotope treatment is frequently combined with ‘cold’ SSA (including non-functioning neoplasms). Monitoring of the effects of PRRT comprises not only imaging examinations, but also monitoring of CgA and the markers specific for functioning PNENs. In PNEN, CgA is a prognostic factor of PFS [148].

Among different forms of PRRT in NETs, including PNENs, radioembolisation with yttrium-labelled microspheres is used, in which the response rate is estimated at 52–66% and the mean survival is 70 months [149, 150]. According to Ramage et al. [151], a complete remission of the disease is achieved in 2.7% of patients, and a partial remission in 60%. Since there are no reports on the use of radioembolisation in a larger group of patients, further studies in this field are necessary.

 

Position of PRRT in the management of PNENs

In both functioning and non-functioning PNENs, the basic form of treatment is surgery. In the case of advanced disease and non-functioning neoplasms, the treatment with labelled somatostatin analogues may be considered as the first-line therapy of disease progression, preceding other forms of management. Chemotherapy may be introduced as the second-line treatment if the disease progresses, especially when SSTR expression is lost.

The next stage in the management of secreting tumours progressing after the surgical treatment is using ‘cold’ SSA. Isotope treatment should be considered as the second-line treatment.

 

Minimal consensus on PRRT PRRT may be used in advanced, inoperable PNENs, especially G2 and G1 (*evidence level 3). PRRT may be considered in individual cases with NEC, provided a high somatostatin receptor expression is confirmed and other forms of therapy prove ineffective (*evidence level 4).

In functioning neoplasms of the pancreas, PRRT as the second line treatment, after a ‘cold’ SSA (*evidence level 3).

In non-functioning tumours of the pancreas, PRRT may be considered as the first-line therapy, preceding other forms of palliative treatment (*evidence level 4).

In both cases, surgical management is the basic form of treatment.

5. Follow-up

This is discussed in detail in section I of "Diagnostic and therapeutic guidelines for gastro-entero-pancreatic neuroendocrine neoplasms (recommended by the Polish Network of Neuroendocrine Tumors)" (pp. 418–443).

 

Minimal consensus on follow-up Monitoring of the treatment should be individualised according to histological differentiation of the NEN (G1, G2 or G3) and the disease staging.

Follow-up comprises clinical examination, determination of the concentration of CgA and specific markers (in functioning neoplasms, depending on the clinical symptoms), as well as radiological (USG, CT/MRI), endoscopic and functioning (SRS) examinations. The frequency of examinations depends on the stage of the disease (2–3 months for NECs and 6–12 months for G1 and G2 PNETs).

References

1. Oberg K, Knigge U, Kwekkeboom D et al. Neuroendocrine gastroentero-pancreatic tumors: ESMO Clinical Practice Guidelines for diagnosis, treatment and follow-up. Ann Oncol 2012; 23 (Suppl.): 124–130.

2. Falconi M, Bartsch DK, Eriksson B et al. ENETS consensus guidelines for the management of patients with digestive neuroendocrine neoplasms of the digestive system: well-differentiated pancreatic non-functioning tumors. Neuroendocrinology 2012; 95: 120–134.

3. Vagefi PA, Razo O, Deshpande V et al. Evolving patterns in the detection andoutcomesofpancreaticneuroendocrineneoplasms:theMassachussets GeneralHospitalexperiencefrom1977to2005.ArchSurg2007;142:347–354.

4. Jensen RT, Cadiot G, Brandi ML et al. ENETS consensus guidelines for the management of patients with digestive neuroendocrine neoplasms: functioning pancreatic endocrine tumor syndromes. Neuroendocrinology 2012; 95: 98–119.

5. Le Roith D. Tumor-induced hypoglycemia. N Engl J Med 1999; 341: 757–758.

6. Oberg K. Pancreatic endocrine tumors. Semin Oncol 2010; 37: 594–618.

7. Vanderveen K, Grant C. Insulinoma. Cancer Treat Res 2010; 153: 235–252.

8. Zhao YP, Zhan HX, Zhang TP et al. Surgical management of patients with insulinomas: result of 292 cases in a single institution. J Surg Oncol 2011; 103: 169–174.

9. Placzkowski KA, Vella A, Thompson GB et al. Secular trends in the presentation and management of functioning insulinoma et the Mayo Clinic, 1987–2007. J Clin Endocrinol Metab 2009; 94: 1069–73.

10. de Herder WW, Niederle B, Scoazec JY et al. Well-differentiated pancreatic tumor/carcinoma: insulinoma. Neuroendocrinology 2006; 84: 183–188.

11. Mathur A, Gorden P, Libutti SK. Insulinoma. Surg Clin North Am 2009; 89: 1105–1121.

12. Marek B, Kajdaniuk D, Kos-Kudła B et al. Insulinoma — diagnosis and treatment. Endokrynol Pol 2007; 58: 58–62.

13. Jonkers YM, Claessen SM, Peren A et al. DNA copy number status is powerful predictor of poor survival in endocrine pancreatic tumor patients. Endocr Relat Cancer 2007; 14: 769–779.

14. Kulke MH, Anthony LB, Bushnell DL et al. NANETS treatment guidelines: well differentiated neuroendocrine tumors of the stomach and pancreas. Pancreas 2010; 39: 735–752.

15. Ekeblad S. Islet cell tumours. Adv Exp Med Biol 2010; 654: 771–789.

16. O’Toole D, Salazar R, Falconi M at al. Rare functioning pancreatic endocrine tumors. Neuroendocrinology 2006; 84: 189–195.

17. Oberg K, Eriksson B. Endocrine tumours of the pancreas. Best Pract Res Clin Gastroenterol 2005; 19: 753–781.

18. Warner RR. Enteroendocrine tumors other than carcinoid: a review clinically significant advances. Gastroenterology 2005; 128: 1668–1684.

19. Metz DC, Jensen RT. Gastrointestinal neuroendocrine tumors: Pancreatic endocrine tumors. Gastroenterology 2008; 135: 1469–1492.

20. Halfdanarson TR, Rabe KG, Rubin J et al. Pancreatic neuroendocrine tumors (PNETs): incidence, prognosis and recent trend toward improved survival. Ann Oncol 2008; 19: 1727–1733.

21. Yao JC, Hassan M, Phan A et al. One hundred years after carcinoid: epidemiology and prognostic factors for neuroendocrine tumors in 35825 cases in the United States. J Clin Oncol 2008; 26: 3063–3072.

22. Triponez F, Dosseh D, Goudet P et al. Epidemiology data on 108 MEN1 patients from the GTE with isolated nonfunctioning tumors of the pancreas. Ann Surg 2006; 243: 265–272.

23. Blansfield JA, Choyke L, Morita SY et al. Clinical, genetic and radiographic analysis of 108 patients with von Hippel-Lindau disease (VHL) manifested by pancreatic neuroendocrine neoplasms (PNETs). Surgery 2007; 142: 814–818.

24. Cheslyn-Curtis S, Sitaram V, Wiliamson RC. Management of nonfunctioning neuroendocrine tumours of the pancreas. Br J Surg 1993; 80: 625–627.

25. Madura JA, Cummings OW, Wiebke EA et al. Nonfunctioning islet cell tumors of the pancreas:a difficult diagnosis but one worth the effort. Am Surg 1997; 63: 573–577.

26. Chu QD, Hill HC, Douglass HO et al. Predictive factors associated with long-term survival in patients with neuroendocrine tumors of the pancreas Ann Surg Oncol 2002; 9: 855–862.

27. Zerbi A, Falconi M, Rindi G et al. Clinicopathological features of pancreatic endocrine tumors: a prospective multicenter study in Italy of 297 sporadic cases. Am J Gastroenterol 2010; 105: 1421–1429.

28. Garcia-Carbonero R, Capdevila J, Crespo-Herrero G et al. Incidence, patterns of care and prognostic factors for outcome of gastroenteropancreatic neuroendocrine tumors (GRP-NETs); results from the National Cancer Registry of Spain (RGETNE). Ann Oncol 2010; 21: 1794–1803.

29. Kazanjian KK, Reber HA, Hines OJ. Resection of pancreatic neuroendocrine tumors: results of 70 cases. Arch Surg 2006; 141: 765–770.

30. Pape UF, Bohmig M, Berndt U et al. Survival and clinical outcome of patients with neuroendocrine tumors of the gastroenteropancreatic tract in a German referral center. Ann NY Acad Sci 2004; 1014: 222–233.

31. Ferrone CR, Tang LH, Tomlinson J et al. Determining prognosis in patients with pancreatic endocrine neoplasms: can the WHO classification system be simplified? J Clin Oncol 2007; 25: 5609–5615.

32. Bilimoria KY, Talamonti MS, Tomlinson JS et al. Prognostic score predicting survival after recection of pancreatic neuroendocrine tumors: analysis of 3851 patient. Ann Surg 2008; 247: 490–500.

33. Mignon M. Natural history of neuroendocrine enteropancreatic tumors. Digestion 200; 62 (Suppl. 1): 51–58.

34. Kos-Kudła B, Bolanowski M, Hubalewska-Dydejczyk A et al. Pancreatic endocrine tumors — management guidelines(recommended by the Polish Network of Neuroendocrine Tumors). Endokrynol Pol 2008; 59: 68–86).

35. Lamberts SW, Hofland LJ, Nobels FR. Neuroendocrine tumor markers. Front Neuroendocrinol 2001; 22: 309–339.

36. Stridsberg M, Eriksson B, Fellstrom B et al. Measurements of chromogranin B can serve as a complement to chromogranin A. Regul Pept 2007; 139: 80–83.

37. Kos-Kudła B, Zemczak A. Diagnostyka biochemiczna guzów neuroendokrynnych układu pokarmowego. In: Kos-Kudła B. (ed.). Guzy neroendokrynne układu pokarmowego. Via Medica, Gdańsk 2010: 17–24.

38. Blicharz-Dorniak J, Kos-Kudła B, Foltyn W et al. Is determination of matrix metalloproteinases and their tissue inhibitors serum concentrations useful in patients with gastroenteropancreatic and bronchopulmonary neuroendocrine neoplasms? Endokrynol Pol 2012; 63: 470–476.

39. Oberg K, Stridsberg M. Chromogranins as diagnostic and prognostic markers in neuroendocrine tumours. Adv Exp Med Biol 2000; 482: 329–337.

40. Halfdanarson TR, Rubin J, Farnell MB et al. Pancreatic endocrine neoplasms: epidemiology and prognosis of pancreatic endocrine tumors. Endocr Relat Cancer 2008; 15: 409–427.

41. Ilias I, Torpy DJ, Pacak K et al. Cushing’s syndrome due to ectopic corticotropin secretion: twenty years’ experience at the National Institutes of Health. J Clin Endocrinol Metab 2005; 90: 4955–4962.

42. Cryer PE, Axelrod L, Grossman AB et al. Evaluation and management of adult hypoglycemic disorders: an Endocrine Society Clinical Practice Guideline. J Clin Endocrinol Metab 2009; 94: 709–728.

43. Ardill JE. Circulating markers for endocrine tumours of the gastroenteropancreatic tract. Ann Clin Biochem 2008; 45: 539–559.

44. Ramage JK, Ahmed A, Ardill J et al. Guidelines for the management of gastroenteropancreatic neuroendocrine (including carcinoid) tumours (NETs). Gut 2012; 61: 6–32.

45. Rindi G, Klőpell G, Couvelard A et al. TNM staging of midgut and hindgut (neuro) endocrine tumors: a consensus proposal including a trading system. Virchows Arch 2007; 451: 757–762.

46. Bosman FT, Carneiro F, Hruban RH, Theise ND (eds.). WHO Classification of Tumours of the Digestive System. IARC, Lyon 2010: 13–14.

47. Kloppel G, Rindi G, Perren A et al. The ENETS and AJCC/UICC TNM classifications of the neuroendocrine tumors of the gastrointestinal tract and the pancreas: a statement. Virchows Arch 2010; 456: 595–597.

48. Kvols LK, Brendtro KL. The North American Neuroendocrine Tumor Society (NANETS) Guidelines. Pancreas 2010; 39: 705–706.

49. Sobin LH, Gospodarowicz MK, Wittekind C. UICC: TNM classification of malignant tumours, 7 th edn. Wiley-Blackwell, Oxford 2009.

50. Klimstra DES, Modlin IR, Adsay NV et al. Pathology reporting of neuroendocrine tumors: Application of the Delphic Consensus process to the development of a minimum pathology data set. Am J Surg Pathol 2010; 34: 300–313.

51. Nasierowska-Guttmejer A. Przyczyny opóźnionej diagnostyki i leczenia guzów neuroendokrynnych trzustki. Komentarz. Przegląd Gastroenterologiczny 2009; 4: 221–223.

52. CapelliP,MartignoniG,PedicaFetal.Endocrineneoplasmsofthepancreas: pathologic and genetic features. Arch Pathol Lab Med 2009; 133: 350–364.

53. Foltyn W, Zajęcki W, Marek B et al. The value of the Ki-67 proliferation marker as a prognostic factor in gastroenteropancreatic neuroendocrine tumours. Endokrynol Pol 2012; 63: 362–366.

54. Kaltsas G, Rockall A, Papadogias D et al. Recent advances in radiological and radionuclide imaging and therapy of neuroendocrine tumours. Eur J Endocrinol 2004; 151: 15–27.

55. Zimmer T, Stolzel U, Bader M et al. Endoscopic ultrasonography and somatostatin receptor scintigraphy in the preoperative localisation of insulinomas and gastrinomas. Gut 1996; 39: 562–568.

56. Ramage JK, Davies AH, Ardill J et al. Guidelines for the management of gastroenteropancreatic neuroendocrine (including carcinoid) tumours. Gut 2005; 54 (Suppl. 4): iv1–iv16.

57. Hawes RH, Fockens P. Endosonography. Saunders Elsevier, Philadelphia, USA 2006.

58. Goldberg J, Rosenblat J, Khatri G et al. Complementary roles of CT and endoscopic ultrasound in evaluating a pancreatic mass. AJR 2010; 194: 984–992.

59. Rösch T, Lightdale CJ, Botet JF et al. Localization of pancreatic endocrine tumors by endoscopic ultrasonography. N Engl J Med 1992; 326: 1721–1726.

60. Pitre J, Soubrane O, Palazzo L et al. Endoscopic ultrasonography for the preoperative localization of insulinomas. Pancreas 1996; 13: 55–60.

61. Schumacher B, Lubkay HJ, Frieling T et al. Prospective study on detection of insulinomas by endoscopic ultrasonography. Endoscopy 1996; 28: 273–276.

62. Gouya H, Vignaux O, Augui J et al. CT, endoscopic sonography, and a combined protocol for preoperative evaluation of pancreatic insulinomas. Am J Roentgenol 2003; 181: 987–992.

63. Zimmer T, Scherübl H, Faiss S et al. Endoscopic ultrasonography of neuroendocrine tumours. Digestion 2000; 62 (Suppl. 1): 45–50.

64. Anderson MA, Carpenter S, Thompson NW et al. Endoscopic ultrasound is highly accurate and directs management in patients with neuroendocrine tumours of the pancreas. Am J Gastroenterol 2000; 95: 2271–2277.

65. Ardengh JC, Rosenbaum P, Ganc AJ et al. Role of EUS in the preoperative localization of insulinomas compared with spiral CT. Gastrointest Endosc 2000; 51: 552–555.

66. Ruszniewski P, Amouyal P, Amouyal G et al. Localisation of gastrinomas by endoscopic ultrasonography in patients with Zollinger-Ellison syndrome. Surgery 1995; 117: 629–635.

67. Sugiyama M, Nobutsugu A, Yumi I et al. Differential diagnosis of Benin versus malignant non-functioning islet cell tumours of the pancreas:the roles of EUS and ERCP. Gastrointest Endosc 2002; 55: 115–119.

68. Tio TL, Sie LH, Kallumaris G et al. Staging of ampullary and pancreatic carcinoma: comparison between endosonography and surgery. Gastrointest Endosc 1996; 44: 706–713.

69. Voss M, Hammel P, Molas G et al. Value of endoscopic ultrasound guided fine needle aspiration biopsy in the diagnosis of solid pancreatic masses. Gut 2000; 46: 244–249.

70. Hocke M, Schulze E, Gottschalk P et al. Contrast-enhanced endoscopic ultrasound in discrimination between focal pancreatitis and pancreatic cancer. World J Gastroenterol 2006; 12: 246–250.

71. Xu C, Li Z, Wallace M. Contrast-enhanced harmonic endoscopic ultrasonography in pancreatic diseases. Diagn Ther Endosc 2012, Article ID 786239, doi:10.1155/2012/786239.

72. Lennon AM, Newman N, Makary MA et al. EUS-guided tattooing before laparoscopic distal pancreatic resection (with video). Gastrointest Endosc 2010; 72: 1089–1094.

73. Newman NA, Lennon AM, Edil BH et al. Preoperative endoscopic tattooing of pancreatic body and tail lesions decreases operative time for laparoscopic distal pancreatectomy. Surgery 2010; 148: 371–377.

74. Kuzin NM, Egorov AV, Kondrashin SA et al. Preoperative and intraoperative topographic diagnosis of insulinomas. World J Surg 1998; 22: 593–597.

75. Ricke J, Klose KJ, Mignon M et al. Standardisation of imaging in neuroendocrine tumours: results of a European delphi process. Eur J Radiol 2001; 37: 8–17.

76. ĆwikłaJB,WaleckiJ.Diagnostykaobrazowaguzówneuroendokrynnych trzustki z elementami leczenia radioizotopowego. Przegl Gastroenterol 2006; 1: 31–44.

77. Sahani DV, Banaffini PA, Del Castillo CF et al. Gastroenteropancreatic Neuroendocrine Tumors: Role of Imaging in Diagnosis and management. Radiology 2013; 266: 38–61.

78. Tatsumoto S, Kodama Y, Sakurai Y et al. Pancreatic neuroednocrine neoplasm: correlation between computed tomography enhancement patterns and prognostic factors of surgical and endoscopic ultrasounguided fine-needle aspiration biopsy specimens. Abdom Imaging 2013; 38: 358–66.

79. Delrue L, Blanckaert P, Mertens D et al. Tissue perfusion in pathologies of the pancreas: assessment using 128-slice computed tomography. Abdom Imaging 2012; 37: 595–601 .

80. Pilch-Kowalczyk J, Leszczyński S. Układ trawienny. In: Leszczyński S, Pilch-Kowalczyk J (eds.). Diagnostyka obrazowa. PZWL, Warszawa 2012.

81. Prokop M, Galanski M, Molen AJ et al. Spiral and multislice computer tomography of the body. Thieme, Stuttgart, New York 2001.

82. Gouya H, Vignaux O, Augui J et al. CT, endoscopic sonography, and a combined protocol for preoperative evaluation of pancreatic insulinomas. Am J Roentgenol 2003; 181: 987–992.

83. Gibril F, Reynolds JC, Doppman JL et al. Somatostatin receptor scintigraphy: its sensitivity compared with that of other imaging methods In detecting primary and metastatic gastrinomas. A prospective study. Ann Intern Med 1996; 125: 26–34.

84. Chiti A, Fanti S, Savelli G et al. Comparison of somatostatin receptor imaging, computed tomography and ultrasound in the clinical management of neuroendocrine gastro-entero-pancreatic tumours. Eur J Nucl Med 1998; 25: 1396–1403.

85. Kartalis N, Lindholm TL, Aspelin P et al. Diffusion-weighted magnetic resonance imaging of pancreas tumours. Eur Radiol 2009; 19: 1981–1990.

86. Krenning EP, Kwekkeboom DJ, Bakker WH et al. Somatostatin receptor scintigraphy with [111In-DTPA-D-Phe1]and [123I-Tyr3]-octreotide: the Rotterdam experience with more than 1000 patients. Eur J Nucl Med 1993; 20: 716–731.

87. Hubalewska-Dydejczyk A, Fross-Baron K, Mikolajczak R et al. (99m) Tc-EDDA/HYNIC-octreotate scintigraphy, an efficient method for the detection and staging of carcinoid tumours: results of 3 years’ experience. Eur J Nucl Med Mol Imaging 2006; 33: 1123–1133.

88. Reubi JC, Waser B. Concomitant expression of several peptide receptors in neuroendocrine tumours: molecular basis for in vivo multireceptor tumour targeting. Eur J Nucl Med Mol Imaging 2003; 30: 781–793.

89. de Kerviler E, Cadiot G, Lebtahi R et al. Somatostatin receptor scintigraphy in forty-eight patients with the Zollinger-Ellison syndrome. GRESZE: Groupe d’Etude du Syndrome de Zollinger-Ellison. Eur J Nucl Med 1994; 21: 1191–1197.

90. Schillaci O, Spanu A, Scopinaro F et al. Somatostatin receptor scintigraphy in liver metastasis detection from gastroenteropancreatic neuroendocrine tumors. J Nucl Med 2003; 44: 359–368.

91. Cimitan M, Buonadonna A, Cannizzaro R et al. Somatostatin receptor scintigraphy versus chromogranin A assay in the management of patients with neuroendocrine tumors of different types: clinical role. Ann Oncol 2003; 14: 1135–1141.

92. Gabriel M, Decristoforo C, Maina T et al. 99mTc-N4-[Tyr3]Octreotate Versus 99mTc-EDDA/HYNIC-[Tyr3]Octreotide: an intrapatient comparison of two novel Technetium-99m labeled tracers for somatostatin receptor scintigraphy. Cancer Biother Radiopharm 2004; 19: 73–79.

93. d’Amico A, Szczucka K, Borys D et al. SPECT-CT fusion: a new diagnostic tool for endocrinology. Endokrynol Pol 2006; 57: 71–74.

94. Anderson CJ, Dehdashti F, Cutler PD et al. 64Cu-TETA-Octreotide as a PET imaging agent for patients with neuroendocrine tumors. J Nucl Med 2001; 42: 213–221.

95. Becherer A, Szabo M, Karanikas G et al. Imaging of Advanced Neuroendocrine Tumors with 18F-FDOPA PET. J Nucl Med 2004; 45:1161–1167.

96. Haug A, Auernhammer CJ, Wängler B et al. Intraindividual comparison of 68Ga-DOTA-TATE and 18F-DOPA PET in patients with well-differentiated metastatic neuroendocrine tumours. Eur J Nucl Med Mol Imaging 2009; 36: 765–770.

97. Tessonnier L, Sebag F, Ghander C et al. Limited Value of 18F-F-DOPA PET to Localize Pancreatic Insulin-Secreting Tumors in Adults with Hyperinsulinemic Hypoglycemia. J Clin Endocrinol Metab 2010; 95: 303–307.

98. Benjegärd SA, Forssell-Aronsson E, Wängberg B et al. Intraoperative tumour detection using 111In DTPA-D-Phe-octreotide and scintillation a detector. Eur J Nucl Med 2001; 28: 1456–1462.

99. Hubalewska-Dydejczyk A, Kulig J, Szybiński P. Radio-Guided Surgery (RGS) with the use of 99mTc-EDDA/HYNIC]octreotate in intra-operative detection of neuroendocrine tumours of gastrointestinal tract (GEPNET). Eur J Nucl Med Mol Imaging 2007; 34: 1545–1555.

100. Reubi JC, Waser B. Concomitant expression of serveral peptide receptors in neuroendocrine tumours: molecular basis for in vivo multireceptor tumour targeting. Eur J Nucl Med Mol Imaging 2003; 30: 781–793.

101. Sowa-Staszczak A, Pach D, Mikołajczak R et al. Glucagon-like peptide-1 receptor imaging with [Lys40(Ahx-HYNIC-99mTc/EDDA)NH2]exendin-4 for the detection of insulinoma. Eur J Nucl Med Mol Imaging 2013: 40: 524–531.

102. Wild D, Christ E, Caplin ME et al. Glucagon-like peptide-1 versus somatostatin receptor targeting reveals 2 distinct forms of malignant insulinomas. J Nucl Med 2011; 52: 1073–1078.

103. Jackson JE. Angiography and arterial stimulation venous sampling in the localization of pancreatic neuroendocrine tumours. Clin Endocrinol Metab 2005; 19: 229–239.

104. Jensen RT, Niederle B, Mitry E et al. Gastrinoma (duodenal and pancreatic). Neuroendocrinology 2006; 84: 173–182.

105. Falconi M, Plockinger U, Kwekkeboom DJ et al. Well-differentiated pancreatic nonfunctioning tumors/carcinoma. Neuroendocrinology 2006; 84: 196–211.

106. Watzka FM, Laumen C, Fottner C et al. Resection strategies for neuroendocrine pancreatic neoplasms. Langenbecks Arch Surg 2013; 398: 431–440.

107. Haugvik SP, Labori KJ, Edwin B et al. Surgical treatment of sporadic pancreatic neuroendocrine tumors: a state of the art review. Scientific World Journal 2012; 2012: 357475.

108. Parekh JR, Wang SC, Bergsland EK et al. Lymph node sampling rates and predictors of nodal metastasis in pancreatic neuroendocrine tumor resections: the UCSF experience with 149 patients. Pancreas 2012; 41: 840–844.

109. Shrikhande SV, Sirohi B, Goel M et al. Pancreatic neuroendocrine tumors. Indian J Gastroenterol 2013; 32: 3–17.

110. Zhao YP, Zhan HX, Zhang TP et al. Surgical management of patients with insulinomas: Result of 292 cases in a single institution. J Surg Oncol 2011; 103: 169–74.

111. Milan SA, Yeo CJ. Neuroendocrine tumors of the pancreas. Curr Opin Oncol 2012; 24: 46–55.

112. Akyildiz HY, Mitchell J, Milas M et al. Laparoscopic radiofrequency thermal ablation of neuroendocrine hepatic metastases:long-term follow-up. Surgery 2010; 148: 1288–1293.

113. Pavel M, Baudin E, Couvelard A et al. ENETS Consensus Guidelines for the management of patients with liver and other distant metastases from neuroendocrine neoplasms of foregut, midgut, hindgut, and unknown primary. Neuroendocrinology 2012; 95: 157–176.

114. Kianmanesh R, Ruszniewski P, Rindi G et al. ENETS consensus guidelines for the management of peritoneal carcinomatosis from neuroendocrine tumors. Neuroendocrinology 2010; 91: 333–340.

115. Wiersema MJ, Wiersema LM. Endosonography guided celiac plexus neurolysis. Gastrointest Endosc 1996; 44: 656–662.

116. Jürgensen C, Schuppan D, Neser F et al. EUS-guided alcohol ablation of an insulinoma. Gastrointestinal Endoscopy 2006; 63: 1059–1062.

117. Deprez PH, Claessens A, Borbath I et al. Successful endoscopic ultrasound guided ethanol ablation of a sporadic insulinoma. Acta Gastroenterologica Belgica 2008; 71: 333–337.

118. Muscatiello N, Salcuni A, Macarini L et al. Treatment of a pancreatic endocrine tumor by ethanol injection guided by endoscopic ultrasound. Endoscopy 2008; 40: E258–259.

119. Rosiek V, Kunikowska J, Kos-Kudła B. A non-functioning pancreatic neuroendocrine tumour: a case report. Endokrynol Pol 2012; 63: 59–64.

120. Modlin IM, Pavel M, Kidd M et al. Review article: somatostatin analogues in the treatment of gastroenteropancreatic neuroendocrine (carcinoid) tumours. Aliment Pharmacol Ther 2010; 31: 169–188.

121. Caplin M, Ruszniewski P, Pavel M et al. A randomized, double-blind, placebo-Controlled study of Lanreotide Antiproliferative Response in patients with gastroenteropancreatic NeuroEndocrine Tumours (CLARINET). EJC 2013; 49 (Suppl. 3): S3.

122. Jawiarczyk A, Bolanowski M, Syrycka J et al. Effective therapy of insulinoma by using long-acting somatostatin analogue. A case report and literature review. Exp Clin Endocrinol Diabetes 2012; 120: 68–72.

123. Rosiek V, Kos-Kudła B. Guidelines for the management of pancreatic neuroendocrine tumors. Gastroenterol Prakt 2010; 2: 28–34.

124. Nikou GC, Toubanakis C, Nikolaou P et al. VIPomas: an update in diagnosis and management in a series of 11 patients. Hepatogastroenterology 2005; 52: 1259–1265.

125. Tomassetti P, Migliori M, Corinaldesi R et al. Treatment of gastroenteropancreatic neuroendocrine tumours with octreotide LAR. Aliment Pharmacol Ther 2000; 14: 557–560.

126. Srirajaskanthan R, McStay M, Toumpanakis C et al. Parathyroid hormone-related peptide-secreting pancreatic neuroendocrine tumours: case series and literature review. Neuroendocrinology 2009; 89: 48–55.

127. Rinke A, Muller HH, Schade-Brittinger C et al. Placebo-Controlled, Double-Blind, Prospective, Randomized Study on the Effect of Octreotide LAR in the Control of Tumor Growth in Patients With Metastatic Neuroendocrine Midgut Tumors: A Report From the PROMID Study Group. J Clin Oncol 2009; 27: 4656–4663.

128. Aparicio T, Ducreux M, Baudin E et al. Antitumour activity of somatostatin analogues in progressive metastatic neuroendocrine tumours. Eur J Cancer 2001; 37: 1014–1019.

129. PanzutoF,DiFonzoM,IannicelliEetal.Long-termclinicaloutcomeofsomatostatinanaloguesfortreatmentofprogressive,metastatic,well-differentiated entero-pancreatic endocrine carcinoma. Ann Oncol 2006; 17: 461–466.

130. Shojamanesh H, Gibril F, Louie A et al. Prospective study of the antitumor efficacy of long term octreotide treatment in patients with progressive metastatic gastrinoma. Cancer 2002; 94: 331–343.

131. Martín-Richard M, Massutí B, Pineda E et al. Antiproliferative effects of lanreotide autogel in patients with progressive, well-differentiated neuroendocrine tumours: a Spanish, multicentre, open-label, single arm phase II study. BMC Cancer 2013; 13: 427.

132. Deptała A, Asendrych A, Omyla-Staszewska J, Rzymkowska J. Rola terapii systemowej w leczeniu guzów neuroendokrynnych układu pokarmowego. Przegląd Gastroenterologiczny 2006; 1: 10–15.

133. Moertel CG, Hanley JA, Johnson LA. Streptozocin alone compared with streptozocin plus fluorouracil in the treatment of advanced islet-cell carcinoma. N Eng J Med 1980; 303: 1189–1194.

134. Moertel CG, Lefkopoulo M, Lipsitz S et al. Streptozocin-doxorubicin, streptozocin-fluorouracil or chlorozotocin in the treatment of advanced islet-cell carcinoma. N Eng J Med 1992; 326: 519–523.

135. Vilar E, Salazar R, Pérez-Garcia J et al. Chemotherapy and role of the proliferation marker Ki-67 in digestive neuroendocrine tumors. Endocr Relat Cancer 2007; 14: 221–232.

136. Raymond E, Dahan L, Raoul JL et al. Sunitinib malate for the treatment of pancreatic neuroendocrine tumors. N Engl J Med 2011; 364: 501–513.

137. Yao JC, Shah MH, Ito T et al. Everolimus for advanced pancreatic neuroendocrine tumors. N Engl J Med 2011; 364: 514–523.

138. Wiedenmann B, Pavel M, Kos-Kudla B. From targets to treatments: A review of molecular targets in pancreatic neuroendocrine tumors. Neuroendocrinology 2011; 94: 177–190.

139. Kulke MH, Bergsland EK, Yao JC. Glycemic control in patients with insulinoma treated with everolimus. N Engl J Med 2009; 360: 195–197.

140. Bourcier ME, Sherrod A, DiGuardo M et al. Successful control of intractable hypoglycemia using rapamycin in an 86-year-old man with a pancreatic insulinsecreting islet cell tumor and metastases. J Clin Endocrinol Metab 2009; 94: 3157–3162.

141. De Jong M, Bakker WH, Breeman WA et al. Pre-clinical comparison of [DTPA0] octreotide, [DTPA0, Tyr3]octreotide and [DOTA0, Tyr3] octreotide as carriers for somatostatin receptor-targeted scintigraphy and radionuclide therapy. Int J Cancer 1998; 75: 406–411.

142. Krenning EP, Kwekkeboom DJ, Valkema R et al. Peptide receptor radionuclide therapy. Ann N Y Acad Sci 2004; 1014: 234–245.

143. Frilling A, Weber F, Saner F et al. Treatment with (90)Yand (177)LuDOTATOC in patients with metastatic neuroendocrine tumors. Surgery 2006; 140: 968–976.

144. Sowa-Staszczak A, Pach D, Chrzan R et al. Peptide receptor radionuclide therapy as a potential tool for neoadjuvant therapy in patients with inoperable neuroendocrine tumours (NETs). Eur J Nucl Med Mol Imaging 2011; 38: 1669–1674.

145. Stoeltzing O, Loss M, Huber E et al. Staged surgery with neoadjuvant 90Y-DOTATOC therapy for down-sizing synchronous bilobular hepatic metastases from a neuroendocrine pancreatic tumor. Langenbecks Arch Surg 2010; 395: 185–192.

146. Barber TW, Hofman MS, Thomson BNJ et al. The potential for neoadjuvant peptide receptor chemoradionuclide therapy to render inoperable pancreatic and duodenal neuroendocrine tumours resectable. Eur J Surg Oncol 2012; 38: 64–71.

147. Pach D, Sowa Staszczak A, Kunikowska J et al. Repeated cycles of peptide receptor radionuclide therapy (PRRT) — Results and side-effects of the radioisotope 90Y-DOTA TATE, 177Lu-DOTA TATE or 90Y/177 Lu-DOTA TATE therapy in patients with disseminated NET. Radiother Oncol 2012; 102: 45–50.

148. Pavel ME, Hainsworth JD, Baudin E et al. Everolimus plus octreotide long-acting repeatable for the treatment of advanced neuroendocrine tumours associated with carcinoid syndrome (RADIANT-2): a randomised, placebo-controlled, phase 3 study. N Engl J Med 2011; 364: 514–523.

149. Paprottka P, Hoffmann R, Haug A et al. Radioembolization of symptomatic, unresectable neuroendocrine hepatic metastases using Yttrium-90 microspheres. Cardiovasc Intervent Radiol 2011: 35: 334–342.

150. Bester L, Meteling B, Pocock N et al. Radioembolisation with Yttrium-90 microspheres: An effective treatment modality for unresectable liver metastases. J Med Imaging Radiat Oncol 2013; 57: 72–80.

151. Ramage JK, Ahmed A, Ardill J et al. Guidelines for the management of gastroenteropancreatic neuroendocrine (including carcinoid) tumours (NETs). Gut 2012; 61: 6–32.

152. OCEBM Levels of Evidence Working Group*. "The Oxford 2011 Levels of Evidence". Oxford Centre for Evidence-Based Medicine. http://www.cebm.net/index.aspx?o=5653 * OCEBM Table of Evidence Working Group = Jeremy Howick, Iain Chalmers (James Lind Library), Paul Glasziou, Trish Greenhalgh, Carl Heneghan, Alessandro Liberati, Ivan Moschetti, Bob Phillips, Hazel Thornton, Olive Goddard and Mary Hodgkinson.

List of Participants of the Consensus Conference on the 2013 Guidelines for the Management of Patients with Digestive Neuroendocrine Neoplasms:

Elżbieta Andrysiak-Mamos (Department of Endocrinology, Metabolic Diseases and Internal Diseases, Pomeranian Medical University, Szczecin, Poland), Tomasz Bednarczuk (Department of Internal Medicine and Endocrinology, Medical University of Warsaw, Warsaw, Poland), Jolanta Blicharz-Dorniak (Division of Endocrinology, Medical University of Silesia, Katowice, Poland), Marek Bolanowski (Department of Endocrinology, Diabetology and Isotope Therapy, Medical University of Wroclaw, Wroclaw, Poland), Andrzej Cichocki (Department of Oncological Surgery, Maria Skłodowska-Curie Memorial Cancer Center and Institute of Oncology, Warsaw Branch, Poland), Jarosław B. Ćwikła (Department of Radiology, Faculty of Medical Science, University of Varmia and Masuria, Olsztyn, Poland), Andrzej Deptała (Department of Oncology and Hematology, Central Clinical Hospital of the Ministry of Interior in Warsaw, Warsaw, Poland), Wanda Foltyn (Division of Endocrinology, Department of Pathophysiology and Endocrinology, Medical University of Silesia, Katowice), Daria Handkiewicz-Junak (Department of Nuclear Medicine and Endocrine Oncology, Maria SkłodowskaCurie Memorial Cancer Center and Institute of Oncology, Gliwice Branch, Poland), Marek Hartleb (Department of Gastroenterology and Hepatology, Medical University of Silesia, Katowice, Poland), Michał Jarząb (Department of Radiotherapy and Chemotherapy, Maria Skłodowska-Curie Memorial Cancer Center and Institute of Oncology, Gliwice Branch, Poland), Arkadiusz Jeziorski (Department of Surgical Oncology, Medical University of Lodz, Lodz, Poland), Dariusz Kajdaniuk (Division ofPathophysiology, Department of Pathophysiology and Endocrinology, Medical University of Silesia, Katowice, Poland), Grzegorz Kamiński (Department of Endocrinology and Radioisotopic Therapy, Military Institute of Medicine, Warsaw, Poland), Aldona Kowalska (Department of Endocrinology, Holycross Cancer Centre, Kielce, Poland), Robert Król (Department of General, Vascular and Transplant Surgery, Medical University of Silesia, Katowice, Poland), Leszek Królicki (Nuclear Medicine Department, Medical University of Warsaw, Warsaw, Poland), Jolanta Kunikowska (Nuclear Medicine Department, Medical University of Warsaw, Warsaw,Poland), Dariusz Lange (DepartmentofTumourPathology, Maria Skłodowska-Curie Memorial Cancer Center and Institute of Oncology, Gliwice Branch, Poland), Anna Lewczuk (Department of Endocrinology and Internal Medicine, Medical University of Gdansk, Poland), Magdalena Londzin-Olesik (Division of Endocrinology, Medical University of Silesia, Katowice, Poland), Przemysław Majewski (Department of Clinical Pathomorphology, Poznan University of Medical Sciences, Poznan, Poland), Gabriela Mełeń-Mucha (Department of Immunoendocrinology, Chair of Endocrinology, Medical University of Lodz, Lodz, Poland), Andrzej Nowak (Department of Gastroenterology and Hepatology, Medical University of Silesia, Katowice, Poland), Waldemar Patkowski (Department of General, Transplant and Liver Surgery, Medical University of Warsaw, Warsaw, Poland), Marek Ruchała (Department of Endocrinology, Metabolism and Internal Medicine, Poznan University of Medical Sciences, Poznan, Poland), Sławomir Rudzki (Department of General and Transplant Surgery and Nutritional Treatment, Medical University of Lublin, Lublin, Poland), Philippe Ruszniewski (Department of Gastroenterology, Hospital Beaujon, AP-HP, University Paris VII, Clichy, France), Grażyna Rydzewska (Clinical Department of Internal Medicine and Gastroenterology, Central Clinical Hospital Ministry of Interior, Warsaw, Poland), Teresa Starzyńska (Department of Gastroenterology, Pomeranian Medical University, Szczecin, Poland), Katarzyna Steinhof-Radwańska (Department of Radiology, Medical University of Silesia, Katowice, Poland), Janusz Strzelczyk (Division of Endocrinology, Department of Pathophysiology and Endocrinology, Medical University of Silesia, Katowice, Poland), Wojciech Zajęcki (Division of Endocrinology, Medical University of Silesia, Katowice, Poland), Piotr Zdunowski (Department of Endocrinology, The Centre of Postgraduate Medical Education, Warsaw, Poland), Anna Zemczak (Division of Endocrinology, Medical University of Silesia, Katowice, Poland).

* evidence level according to CEMB [152]
same for every * in this chapter

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