open access

Vol 67, No 1 (2016)
Original papers
Published online: 2016-02-17
Submitted: 2015-08-09
Accepted: 2015-11-16
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The value of the repeated examination of BRAF V600E mutation status in diagnostics of papillary thyroid cancer

Augustas Beiša, Virgilijus Beiša, Mindaugas Stoškus, Elvyra Ostanevičiūtė, Laimonas Griškevičius, Kęstutis Strupas
DOI: 10.5603/EP.2016.0005
·
Endokrynologia Polska 2016;67(1):35-40.

open access

Vol 67, No 1 (2016)
Original papers
Published online: 2016-02-17
Submitted: 2015-08-09
Accepted: 2015-11-16

Abstract

Introduction: Nodular thyroid disease is one of the most frequently diagnosed pathologies of the adult population in iodine-deficient regions. Approximately 30% of thyroid aspirates are classified as nondiagnostic/unsatisfactory or indeterminate. However, patients with indeterminate cytology still undergo surgery. The object of this study was to determine the diagnostic value of re-examining the BRAF V600E mutation in papillary thyroid carcinoma patients.

Material and methods: All patients underwent ultrasound guided fine-needle aspiration of a thyroid nodule. They were assigned to one of the four groups (indeterminate or positive for malignant cells) of the Bethesda System for Reporting Thyroid Cytopathology. Genetic investigation of the BRAF V600E mutation was performed for all of the fine-needle aspiration cytology specimens. All of the patients underwent surgery. Subsequently, histological investigation of the removed tissues was performed. Additional analysis of the BRAF V600E mutation from the histology specimen was then performed for the initially BRAF-negative cases.

Results: Two hundred and fourteen patients were involved in the study. One hundred and six (49.53%) patients were diagnosed with thyroid cancer. Of these 106 patients, 95 (89.62%) patients were diagnosed with papillary thyroid cancer. The BRAF V600E mutation was positive in 62 (65.26%) and negative in 33 (34.74%) histologically confirmed papillary thyroid cancer cases. After the genetic investigation, a total of 74 (77.89%) papillary thyroid cancer cases were positive for the BRAF V600E mutation and 21 (22.11%) were negative.

Conclusions: Repeated examination of the BRAF V600E mutation status in the fine-needle aspiration may potentially increase the sensitivity of papillary thyroid cancer diagnostics. (Endokrynol Pol 2016; 67 (1): 35–40)

Abstract

Introduction: Nodular thyroid disease is one of the most frequently diagnosed pathologies of the adult population in iodine-deficient regions. Approximately 30% of thyroid aspirates are classified as nondiagnostic/unsatisfactory or indeterminate. However, patients with indeterminate cytology still undergo surgery. The object of this study was to determine the diagnostic value of re-examining the BRAF V600E mutation in papillary thyroid carcinoma patients.

Material and methods: All patients underwent ultrasound guided fine-needle aspiration of a thyroid nodule. They were assigned to one of the four groups (indeterminate or positive for malignant cells) of the Bethesda System for Reporting Thyroid Cytopathology. Genetic investigation of the BRAF V600E mutation was performed for all of the fine-needle aspiration cytology specimens. All of the patients underwent surgery. Subsequently, histological investigation of the removed tissues was performed. Additional analysis of the BRAF V600E mutation from the histology specimen was then performed for the initially BRAF-negative cases.

Results: Two hundred and fourteen patients were involved in the study. One hundred and six (49.53%) patients were diagnosed with thyroid cancer. Of these 106 patients, 95 (89.62%) patients were diagnosed with papillary thyroid cancer. The BRAF V600E mutation was positive in 62 (65.26%) and negative in 33 (34.74%) histologically confirmed papillary thyroid cancer cases. After the genetic investigation, a total of 74 (77.89%) papillary thyroid cancer cases were positive for the BRAF V600E mutation and 21 (22.11%) were negative.

Conclusions: Repeated examination of the BRAF V600E mutation status in the fine-needle aspiration may potentially increase the sensitivity of papillary thyroid cancer diagnostics. (Endokrynol Pol 2016; 67 (1): 35–40)

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Keywords

BRAF mutation; thyroid cancer; fine-needle aspiration

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About this article
Title

The value of the repeated examination of BRAF V600E mutation status in diagnostics of papillary thyroid cancer

Journal

Endokrynologia Polska

Issue

Vol 67, No 1 (2016)

Pages

35-40

Published online

2016-02-17

DOI

10.5603/EP.2016.0005

Bibliographic record

Endokrynologia Polska 2016;67(1):35-40.

Keywords

BRAF mutation
thyroid cancer
fine-needle aspiration

Authors

Augustas Beiša
Virgilijus Beiša
Mindaugas Stoškus
Elvyra Ostanevičiūtė
Laimonas Griškevičius
Kęstutis Strupas

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