open access

Vol 64, No 5 (2013)
Original paper
Submitted: 2013-11-04
Accepted: 2013-11-04
Published online: 2013-11-01
Get Citation

Unsupervised analysis of follicular thyroid tumours transcriptome by oligonucleotide microarray gene expression profiling

Bartosz Wojtaś, Aleksandra Pfeifer, Michał Jarząb, Agnieszka Czarniecka, Jolanta Krajewska, Michał Świerniak, Tomasz Stokowy, Dagmara Rusinek, Monika Kowal, Jadwiga Żebracka-Gala, Tomasz Tyszkiewicz, Małgorzata Oczko-Wojciechowska, Ewa Stobiecka, Dariusz Lange, Ralf Paschke, Barbara Jarząb
DOI: 10.5603/EP.2013.0013
·
Endokrynol Pol 2013;64(5):328-334.

open access

Vol 64, No 5 (2013)
Original Paper
Submitted: 2013-11-04
Accepted: 2013-11-04
Published online: 2013-11-01

Abstract

Introduction: Mechanisms driving the invasiveness of follicular thyroid cancer (FTC) are not fully understood. In our study, we undertook
an unsupervised analysis of the set of follicular thyroid tumours (adenomas (FTA) and carcinomas) to verify whether the malignant
phenotype influences major sources of variability in our dataset.
Material and methods: The core set of samples consisted of 52 tumours (27 FTC, 25 FTA). Total RNA was analysed by oligonucleotide
microarray (HG-U133 Plus 2.0). Principal Component Analysis (PCA) was applied as a main method of unsupervised analysis.
Results: An analysis of biological character of genes correlated to the first six PCs was performed. When genes correlated to the first PC
were used to cluster FTC and FTA, they appeared in two branches; one, relatively enriched in adenomas, with homogenous expression
of subset of genes, and the other containing mainly carcinomas, with down-regulation of these genes and heterogeneous up-regulation
in a smaller cluster of transcripts. Genes highly up-regulated in adenomas included some thyroid-specific transcripts. The second cluster
of genes, up-regulated in carcinomas, contained mainly immunity-related transcripts. Immune response genes were found in the first,
third and sixth principal components, improving the discrimination between carcinomas and adenomas.
Conclusions: Our unsupervised analysis indicates that invasiveness of follicular tumours might be considered as the major source of variability
in transcriptome analysis. However, the distance between both groups is small and the clusters are overlapping, thus, unsupervised
analysis is not sufficient to properly classify them. (Endokrynol Pol 2013; 64 (5): 328–334)

Abstract

Introduction: Mechanisms driving the invasiveness of follicular thyroid cancer (FTC) are not fully understood. In our study, we undertook
an unsupervised analysis of the set of follicular thyroid tumours (adenomas (FTA) and carcinomas) to verify whether the malignant
phenotype influences major sources of variability in our dataset.
Material and methods: The core set of samples consisted of 52 tumours (27 FTC, 25 FTA). Total RNA was analysed by oligonucleotide
microarray (HG-U133 Plus 2.0). Principal Component Analysis (PCA) was applied as a main method of unsupervised analysis.
Results: An analysis of biological character of genes correlated to the first six PCs was performed. When genes correlated to the first PC
were used to cluster FTC and FTA, they appeared in two branches; one, relatively enriched in adenomas, with homogenous expression
of subset of genes, and the other containing mainly carcinomas, with down-regulation of these genes and heterogeneous up-regulation
in a smaller cluster of transcripts. Genes highly up-regulated in adenomas included some thyroid-specific transcripts. The second cluster
of genes, up-regulated in carcinomas, contained mainly immunity-related transcripts. Immune response genes were found in the first,
third and sixth principal components, improving the discrimination between carcinomas and adenomas.
Conclusions: Our unsupervised analysis indicates that invasiveness of follicular tumours might be considered as the major source of variability
in transcriptome analysis. However, the distance between both groups is small and the clusters are overlapping, thus, unsupervised
analysis is not sufficient to properly classify them. (Endokrynol Pol 2013; 64 (5): 328–334)
Get Citation

Keywords

follicular thyroid carcinoma; follicular adenoma; gene expression

About this article
Title

Unsupervised analysis of follicular thyroid tumours transcriptome by oligonucleotide microarray gene expression profiling

Journal

Endokrynologia Polska

Issue

Vol 64, No 5 (2013)

Article type

Original paper

Pages

328-334

Published online

2013-11-01

Page views

2081

Article views/downloads

1843

DOI

10.5603/EP.2013.0013

Bibliographic record

Endokrynol Pol 2013;64(5):328-334.

Keywords

follicular thyroid carcinoma
follicular adenoma
gene expression

Authors

Bartosz Wojtaś
Aleksandra Pfeifer
Michał Jarząb
Agnieszka Czarniecka
Jolanta Krajewska
Michał Świerniak
Tomasz Stokowy
Dagmara Rusinek
Monika Kowal
Jadwiga Żebracka-Gala
Tomasz Tyszkiewicz
Małgorzata Oczko-Wojciechowska
Ewa Stobiecka
Dariusz Lange
Ralf Paschke
Barbara Jarząb

Regulations

Important: This website uses cookies. More >>

The cookies allow us to identify your computer and find out details about your last visit. They remembering whether you've visited the site before, so that you remain logged in - or to help us work out how many new website visitors we get each month. Most internet browsers accept cookies automatically, but you can change the settings of your browser to erase cookies or prevent automatic acceptance if you prefer.

Via MedicaWydawcą jest  VM Media Group sp. z o.o., Grupa Via Medica, ul. Świętokrzyska 73, 80–180 Gdańsk

tel.:+48 58 320 94 94, faks:+48 58 320 94 60, e-mail:  viamedica@viamedica.pl