Vol 64, No 3 (2013)
Review paper
Published online: 2013-07-01
Can we accurately measure the concentration of clinically relevant vitamin D metabolites in the circulation? The problems and their consequences
Endokrynol Pol 2013;64(3):238-245.
Abstract
Increased interest in vitamin D measurements in clinical studies has contributed to the development in recent years of several new immunochemical
assays (manual and for automatic analyzers). New methods, including HPLC (high performance liquid chromatography),
and LC-MS/MS (liquid chromatography coupled with tandem mass spectrometry) have also been introduced into routine diagnostic
laboratories. Because of the variety of assays and methods used, the question arises which one is the most accurate for the measurement
of vitamin D metabolites concentration. In this review, we summarise the advantages and disadvantages of these methods, describe
the complexity of vitamin D metabolites pattern in the circulation, and discuss the problem of accurate measuring its concentration.
(Endokrynol Pol 2013; 64 (3): 238–245)
Keywords: vitamin DcalcidiolcalcitriolimmunoassaysLC-MS/MS