open access

Vol 64, No 2 (2013)
Original papers
Published online: 2013-04-30
Submitted: 2013-05-14
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Correlations between polymorphisms in genes coding elements of dopaminergic pathways and body mass index in overweight and obese women

Marcin Sikora, Anna Gese, Ryszard Czypicki, Marcin Gąsior, Andrzej Tretyn, Jacek Chojnowski, Maciej Bieliński, Marcin Jaracz, Anna Kamińska, Roman Junik, Alina Borkowska
Endokrynologia Polska 2013;64(2):101-107.

open access

Vol 64, No 2 (2013)
Original papers
Published online: 2013-04-30
Submitted: 2013-05-14

Abstract


Introduction: Dopamine is considered to be crucial for food craving and intake, drug abuse and electrical brain stimulation. Increased levels of dopamine occur after energy intake in the dorsal striatum. In the ventral tagmental area, dopamine is responsible for motivation. There is a natural synaptic dopamine level, and as a result its activity is controlled by density of receptors, amount of released neurotransmitter, and defectiveness of re-uptake by specific transporters. In our study, we wanted to investigate if there is a correlation between mean BMI values and VNTR polymorphisms in SLC6A3 (rs28363170) and DRD4 genes.
Material and methods: Chosen gene fragments were amplified using polymerase chain reaction on the DNA template obtained from 506 women. The products of the reaction were electrophoresed and visualised in 3% agarose gel. The genotyping data was analysed with Kruskal-Wallis tests (p < 0.05).
Results: In the case of SLC6A3, statistically significant differences in mean BMI were found in the group of obese women (p < 0.05) but not for the whole population of women with normal weight or with overweight (p > 0.05). The mean BMI was higher for the SS genotype than for combined LL and LS genotypes. The difference in mean BMI values for variants of DRD4 was significant for the whole studied population and in the obese group (p > 0.05), and the higher value was correlated with the presence of a variant with seven or more repeats of 48 bp motif.
Conclusions: When the two analysed polymorphisms were combined, the spread between the mean BMI values became greater than for single genes. This suggests that the effect on body mass of these two polymorphisms may combine and cause hypo-functionality of the dopaminergic reward system. (Endokrynol Pol 2013; 64 (2): 101–107)

Abstract


Introduction: Dopamine is considered to be crucial for food craving and intake, drug abuse and electrical brain stimulation. Increased levels of dopamine occur after energy intake in the dorsal striatum. In the ventral tagmental area, dopamine is responsible for motivation. There is a natural synaptic dopamine level, and as a result its activity is controlled by density of receptors, amount of released neurotransmitter, and defectiveness of re-uptake by specific transporters. In our study, we wanted to investigate if there is a correlation between mean BMI values and VNTR polymorphisms in SLC6A3 (rs28363170) and DRD4 genes.
Material and methods: Chosen gene fragments were amplified using polymerase chain reaction on the DNA template obtained from 506 women. The products of the reaction were electrophoresed and visualised in 3% agarose gel. The genotyping data was analysed with Kruskal-Wallis tests (p < 0.05).
Results: In the case of SLC6A3, statistically significant differences in mean BMI were found in the group of obese women (p < 0.05) but not for the whole population of women with normal weight or with overweight (p > 0.05). The mean BMI was higher for the SS genotype than for combined LL and LS genotypes. The difference in mean BMI values for variants of DRD4 was significant for the whole studied population and in the obese group (p > 0.05), and the higher value was correlated with the presence of a variant with seven or more repeats of 48 bp motif.
Conclusions: When the two analysed polymorphisms were combined, the spread between the mean BMI values became greater than for single genes. This suggests that the effect on body mass of these two polymorphisms may combine and cause hypo-functionality of the dopaminergic reward system. (Endokrynol Pol 2013; 64 (2): 101–107)
Get Citation

Keywords

dopamine; SLC6A3; DRD4; obesity

About this article
Title

Correlations between polymorphisms in genes coding elements of dopaminergic pathways and body mass index in overweight and obese women

Journal

Endokrynologia Polska

Issue

Vol 64, No 2 (2013)

Pages

101-107

Published online

2013-04-30

Bibliographic record

Endokrynologia Polska 2013;64(2):101-107.

Keywords

dopamine
SLC6A3
DRD4
obesity

Authors

Marcin Sikora
Anna Gese
Ryszard Czypicki
Marcin Gąsior
Andrzej Tretyn
Jacek Chojnowski
Maciej Bieliński
Marcin Jaracz
Anna Kamińska
Roman Junik
Alina Borkowska

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