open access

Vol 56, No 1 (2005)
Original papers
Published online: 2006-03-22
Submitted: 2013-02-15
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Estimation of sodium/iodide symporter gene expression (NIS) in thyroid cancer by RT-PCR technique (preliminary study)

Milena Tosiek, Lech Pomorski, Ewa Balcerczak, Marek Mirowski, Wojciech Czyż
Endokrynologia Polska 2005;56(1):25-29.

open access

Vol 56, No 1 (2005)
Original papers
Published online: 2006-03-22
Submitted: 2013-02-15

Abstract

NIS gene is located on chromosome 19 and encodes 643 amino acid protein. It belongs to membrane Na+ dependent glucose symporter proteins family. In normal thyroid is located in basolateral membrane of thyreocyte. It plays a main role in concentrating of iodine in thyreocyte and thus in thyroid hormones synthesis. It was proved that NIS expression influences effectiveness of radioactive iodine therapy in well-differentiated thyroid cancers. The aim of this study was to estimate the NIS expression and its dependence with gender, age and stage in thyroid papillary and follicular cancers. The frozen sections of tissue were used as a source of tumor RNA. RT-PCR technique was employed for NIS expression analysis. We did not find dependence between the presence of NIS expression in investigated thyroid cancers and stage of disease estimated according to TNM classification. We also did not find dependence between NIS expression and gender or sex of the patients. Our results suggest that there is no dependence between NIS expression and iodine uptake.

Abstract

NIS gene is located on chromosome 19 and encodes 643 amino acid protein. It belongs to membrane Na+ dependent glucose symporter proteins family. In normal thyroid is located in basolateral membrane of thyreocyte. It plays a main role in concentrating of iodine in thyreocyte and thus in thyroid hormones synthesis. It was proved that NIS expression influences effectiveness of radioactive iodine therapy in well-differentiated thyroid cancers. The aim of this study was to estimate the NIS expression and its dependence with gender, age and stage in thyroid papillary and follicular cancers. The frozen sections of tissue were used as a source of tumor RNA. RT-PCR technique was employed for NIS expression analysis. We did not find dependence between the presence of NIS expression in investigated thyroid cancers and stage of disease estimated according to TNM classification. We also did not find dependence between NIS expression and gender or sex of the patients. Our results suggest that there is no dependence between NIS expression and iodine uptake.
Get Citation

Keywords

sodium/iodide gene expression; thyroid cancer

About this article
Title

Estimation of sodium/iodide symporter gene expression (NIS) in thyroid cancer by RT-PCR technique (preliminary study)

Journal

Endokrynologia Polska

Issue

Vol 56, No 1 (2005)

Pages

25-29

Published online

2006-03-22

Bibliographic record

Endokrynologia Polska 2005;56(1):25-29.

Keywords

sodium/iodide gene expression
thyroid cancer

Authors

Milena Tosiek
Lech Pomorski
Ewa Balcerczak
Marek Mirowski
Wojciech Czyż

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