open access

Vol 56, No 1 (2005)
Original papers
Published online: 2006-03-22
Submitted: 2013-02-15
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Production and preliminary characteristics of polyclonal antibodies specific to SSTR2A and SSTR5 receptors in pituitary

Zbigniew Bartoszewicz, Anna Baran, Anna M. Makowska, Ewa Matyja, Ewa Bar-Andziak
Endokrynologia Polska 2005;56(1):7-13.

open access

Vol 56, No 1 (2005)
Original papers
Published online: 2006-03-22
Submitted: 2013-02-15

Abstract

The increasing interest in somatostatin receptors (SSTR) is mainly due to their involvement in the regulation of hormone secretion and the role in somatostatin analogue treatment of patients with pituitary adenomas. The efficiency of this treatment is highly dependent on receptor expression in tissue which on the protein level requires specific anti-receptor antibodies for testing.In this paper we introduced the principles for the production of several polyclonal antibodies specific for C-terminal intracellular part or N-terminal extracellular part of SSTR2A and SSTR5 receptors. The antibodies were highly specific for peptides used for immunization of animals, had low cross-reaction activities for other SSTR2A and SSTR5 peptides and reacted with receptors on immunoblots and in immunohistochemistry. The final verification of the antibodies’ specificity would allow us to perform the research concerning the structure and posttranslational modifications of SSTR and avoid dependency of commercial sources.

Abstract

The increasing interest in somatostatin receptors (SSTR) is mainly due to their involvement in the regulation of hormone secretion and the role in somatostatin analogue treatment of patients with pituitary adenomas. The efficiency of this treatment is highly dependent on receptor expression in tissue which on the protein level requires specific anti-receptor antibodies for testing.In this paper we introduced the principles for the production of several polyclonal antibodies specific for C-terminal intracellular part or N-terminal extracellular part of SSTR2A and SSTR5 receptors. The antibodies were highly specific for peptides used for immunization of animals, had low cross-reaction activities for other SSTR2A and SSTR5 peptides and reacted with receptors on immunoblots and in immunohistochemistry. The final verification of the antibodies’ specificity would allow us to perform the research concerning the structure and posttranslational modifications of SSTR and avoid dependency of commercial sources.
Get Citation

Keywords

somatostatin receptors; polyclonal antibodies; Western blotting; immunohistochemistry

About this article
Title

Production and preliminary characteristics of polyclonal antibodies specific to SSTR2A and SSTR5 receptors in pituitary

Journal

Endokrynologia Polska

Issue

Vol 56, No 1 (2005)

Pages

7-13

Published online

2006-03-22

Bibliographic record

Endokrynologia Polska 2005;56(1):7-13.

Keywords

somatostatin receptors
polyclonal antibodies
Western blotting
immunohistochemistry

Authors

Zbigniew Bartoszewicz
Anna Baran
Anna M. Makowska
Ewa Matyja
Ewa Bar-Andziak

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