Vol 56, No 2 (2005)
Original paper
Published online: 2006-03-24
Serum concentrations of tumor necrosis factor TNFα and its soluble receptors in obese women with diabetes type 2 and without additional disease
Endokrynol Pol 2005;56(2):174-178.
Abstract
Introduction: TNF-α is one with mediators insulin resistance. Previous study showed, that in obesity there is an increased synthesis of TNF-α by fat cells and serum concentrations of TNF-α.
The aim of present study was: 1. To assess of serum concentrations of TNF-α and TNF soluble receptors sTNFRs in obese women with diabetes type 2 and obese women without additional disease. 2. To assess possible association between of manner treatment of diabetes type 2 and serum concentrations of TNF-α and TNF soluble receptors.
Material and methods: The study group’s involved 23 obese women with diabetes type 2 - group A (age 63.6 ± 8.2 lat; BMI 32.7 ± 3,9 kg/m2) in this 12 treated of derivatives of sulfonylurea (age 65.1 ± 6.6 lat; BMI 32.0 ± 3.4 kg/m2) - subgroup AI and 11 insulin treated (age 62.1 ± 9.7 lat; BMI 33.4 ± 4.4 kg/m2) – subgroup AII and 23 obese women without additional disease and without any pharmacological treatment - group B (age 36.6 ± 10.9 lat; BMI 36.6 ± 5.6 kg/m2). Body weight and height were measured, body mass index was calculated with formula. Serum concentrations of glucose was measured by enzymatic procedure. Serum concentrations of TNF-α and it’s soluble receptors sTNFR1 and sTNFR2 was measured by ELISA.
Results: Serum concentration of TNF-α was significant increased p < 0,005, however concentrations of sTNFR1 and sTNFR2 were significant decreased (respectively p < 0,005 i p < 0,001) in group A when compared to group B. There are not significant differences serum concentration of TNF-α and its soluble receptors between subgroups AI and AII.
Conclusions: 1. In obese women with diabetes type 2 serum concentration of TNF-α increased and concentrations of its soluble receptors decreased when compared to obese without additional disease. 2. The treatment meaner of diabetes type 2 not influence of serum concentration of TNF-α and sTNFR1 but application of insulin maybe a cause increase activity sTNFR2.
The aim of present study was: 1. To assess of serum concentrations of TNF-α and TNF soluble receptors sTNFRs in obese women with diabetes type 2 and obese women without additional disease. 2. To assess possible association between of manner treatment of diabetes type 2 and serum concentrations of TNF-α and TNF soluble receptors.
Material and methods: The study group’s involved 23 obese women with diabetes type 2 - group A (age 63.6 ± 8.2 lat; BMI 32.7 ± 3,9 kg/m2) in this 12 treated of derivatives of sulfonylurea (age 65.1 ± 6.6 lat; BMI 32.0 ± 3.4 kg/m2) - subgroup AI and 11 insulin treated (age 62.1 ± 9.7 lat; BMI 33.4 ± 4.4 kg/m2) – subgroup AII and 23 obese women without additional disease and without any pharmacological treatment - group B (age 36.6 ± 10.9 lat; BMI 36.6 ± 5.6 kg/m2). Body weight and height were measured, body mass index was calculated with formula. Serum concentrations of glucose was measured by enzymatic procedure. Serum concentrations of TNF-α and it’s soluble receptors sTNFR1 and sTNFR2 was measured by ELISA.
Results: Serum concentration of TNF-α was significant increased p < 0,005, however concentrations of sTNFR1 and sTNFR2 were significant decreased (respectively p < 0,005 i p < 0,001) in group A when compared to group B. There are not significant differences serum concentration of TNF-α and its soluble receptors between subgroups AI and AII.
Conclusions: 1. In obese women with diabetes type 2 serum concentration of TNF-α increased and concentrations of its soluble receptors decreased when compared to obese without additional disease. 2. The treatment meaner of diabetes type 2 not influence of serum concentration of TNF-α and sTNFR1 but application of insulin maybe a cause increase activity sTNFR2.
Keywords: TNF-αsoluble receptors of TNFdiabetes type 2obesity
