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Evaluation of the effect of GHRH(1-44)NH2 on the secretion of interleukin-2 (IL-2) and soluble IL-2 receptor α (sIL-2Rα) from human peripheral blood mononuclear cells in vitro
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Abstract
Aim: The aim of the study was to examine the influence of GHRH on IL -2 as well as sIL -2Rα secretion from mitogen-stimulated peripheral blood mononuclear cells.
Material and method: Mononuclear cells (PBMC) were isolated from the peripheral blood of healthy adults according to the technique described by Böyum. Cells, cultured 24 hours at 370 C in humudified atmosphere of 95% air and 5% CO2, were stimulated with phytohemaglutinin (PHA; 10 µg/ml), and then GHRH(1-44)NH2 at the final concentrations 10-12, 10-10, 10-8 and 10-6 M was added to appropriate wells. ELISA kits were used to measure IL-2 and sIL-2Rα concentrations in the supernatants of cultured cells. Comparisons between tested groups were made by U Mann-Whitney test. The differences were considered significant if p < 0.05.
Results: GHRH stimulated the secretion of IL -2 into the supernatants acting significantly at the concentration of 10-12M (p < 0.001). Moreover, GHRH at concentrations 10-10M and 10-8M significantly increased the secretion of sIL-2Rα as well (p < 0.001). Strong positive correlation between tested GHRH concentrations and sIL-2Rα levels in the supernatants was demonstrated (r = 0.8664; p < 0.001).
Conclusions: The results demonstrate the potential involvement of GHRH in the regulation of T lymphocytes secretory function.
Abstract
Aim: The aim of the study was to examine the influence of GHRH on IL -2 as well as sIL -2Rα secretion from mitogen-stimulated peripheral blood mononuclear cells.
Material and method: Mononuclear cells (PBMC) were isolated from the peripheral blood of healthy adults according to the technique described by Böyum. Cells, cultured 24 hours at 370 C in humudified atmosphere of 95% air and 5% CO2, were stimulated with phytohemaglutinin (PHA; 10 µg/ml), and then GHRH(1-44)NH2 at the final concentrations 10-12, 10-10, 10-8 and 10-6 M was added to appropriate wells. ELISA kits were used to measure IL-2 and sIL-2Rα concentrations in the supernatants of cultured cells. Comparisons between tested groups were made by U Mann-Whitney test. The differences were considered significant if p < 0.05.
Results: GHRH stimulated the secretion of IL -2 into the supernatants acting significantly at the concentration of 10-12M (p < 0.001). Moreover, GHRH at concentrations 10-10M and 10-8M significantly increased the secretion of sIL-2Rα as well (p < 0.001). Strong positive correlation between tested GHRH concentrations and sIL-2Rα levels in the supernatants was demonstrated (r = 0.8664; p < 0.001).
Conclusions: The results demonstrate the potential involvement of GHRH in the regulation of T lymphocytes secretory function.
Keywords
interleukin-2; interleukin-2 receptor; somatoliberin; neuroimmunomodulation
About this articleTitle
Evaluation of the effect of GHRH(1-44)NH2 on the secretion of interleukin-2 (IL-2) and soluble IL-2 receptor α (sIL-2Rα) from human peripheral blood mononuclear cells in vitro
Journal
Issue
Article type
Original paper
Pages
773-780
Published online
2006-05-28
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529
Article views/downloads
1973
Bibliographic record
Endokrynol Pol 2005;56(5):773-780.
Keywords
interleukin-2
interleukin-2 receptor
somatoliberin
neuroimmunomodulation
Authors
Agnieszka Siejka
Tomasz Stępień
Hanna Ławnicka
Roman Krupiński
Jan Komorowski
Henryk Stępień
