Vol 56, No 5 (2005)
Original paper
Published online: 2006-05-28
Gene expression analysis by DNA microarray in papillary thyroid cancer
Endokrynol Pol 2005;56(5):752-757.
Abstract
In our study we present chosen elements of microarray
analysis of gene expression profile in papillary thyroid
cancer. The study group included 16 papillary thyroid
cancer tissues and 16 corresponding normal tissues.
Samples were analyzed on high density oligonucleotide
microarrays (GeneChip HG-U133A) which contain
22.000 genes. 110 genes, which had significant changed
expression, were selected by MAS 5.0 program. 3 genes
were chosen to the deeper analysis: dipeptidylpeptidase
4 (DPP4), fibronectin 1 (FN1), tissue inhibitor of
metalloproteinase 1 (TIMP1). DPP4-RNA were absent
in normal tissue while in cancer tissue it was detected in
large amount. FN1 and TIMP1 expression were detected
in normal tissue but markedly increased in papillary
thyroid cancer. Among these 3 genes DPP4 seems to be
the best molecular marker for papillary thyroid cancer.
Keywords: microarrayspapillary thyroid cancergene
