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Vol 56, No 5 (2005)
Original paper
Submitted: 2013-02-15
Published online: 2006-05-28
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Gene expression analysis by DNA microarray in papillary thyroid cancer

Elżbieta Gubała, Małgorzata Wiench, Małgorzata Oczko-Wojciechowska, Dariusz Lange, Jan Włoch, Agnieszka Czarniecka, Ewa Chmielik, Barbara Nikiel, Zbigniew Wygoda, Aleksandra Kukulska, Daria Handkiewicz-Junak, Sylwia Szpak-Ulczok, Aleksandra Krawczyk, Józef Roskosz, Barbara Jarząb
Endokrynol Pol 2005;56(5):752-757.

open access

Vol 56, No 5 (2005)
Original Paper
Submitted: 2013-02-15
Published online: 2006-05-28

Abstract

In our study we present chosen elements of microarray analysis of gene expression profile in papillary thyroid cancer. The study group included 16 papillary thyroid cancer tissues and 16 corresponding normal tissues. Samples were analyzed on high density oligonucleotide microarrays (GeneChip HG-U133A) which contain 22.000 genes. 110 genes, which had significant changed expression, were selected by MAS 5.0 program. 3 genes were chosen to the deeper analysis: dipeptidylpeptidase 4 (DPP4), fibronectin 1 (FN1), tissue inhibitor of metalloproteinase 1 (TIMP1). DPP4-RNA were absent in normal tissue while in cancer tissue it was detected in large amount. FN1 and TIMP1 expression were detected in normal tissue but markedly increased in papillary thyroid cancer. Among these 3 genes DPP4 seems to be the best molecular marker for papillary thyroid cancer.

Abstract

In our study we present chosen elements of microarray analysis of gene expression profile in papillary thyroid cancer. The study group included 16 papillary thyroid cancer tissues and 16 corresponding normal tissues. Samples were analyzed on high density oligonucleotide microarrays (GeneChip HG-U133A) which contain 22.000 genes. 110 genes, which had significant changed expression, were selected by MAS 5.0 program. 3 genes were chosen to the deeper analysis: dipeptidylpeptidase 4 (DPP4), fibronectin 1 (FN1), tissue inhibitor of metalloproteinase 1 (TIMP1). DPP4-RNA were absent in normal tissue while in cancer tissue it was detected in large amount. FN1 and TIMP1 expression were detected in normal tissue but markedly increased in papillary thyroid cancer. Among these 3 genes DPP4 seems to be the best molecular marker for papillary thyroid cancer.

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Keywords

microarrays; papillary thyroid cancer; gene

About this article
Title

Gene expression analysis by DNA microarray in papillary thyroid cancer

Journal

Endokrynologia Polska

Issue

Vol 56, No 5 (2005)

Article type

Original paper

Pages

752-757

Published online

2006-05-28

Page views

682

Article views/downloads

1415

Bibliographic record

Endokrynol Pol 2005;56(5):752-757.

Keywords

microarrays
papillary thyroid cancer
gene

Authors

Elżbieta Gubała
Małgorzata Wiench
Małgorzata Oczko-Wojciechowska
Dariusz Lange
Jan Włoch
Agnieszka Czarniecka
Ewa Chmielik
Barbara Nikiel
Zbigniew Wygoda
Aleksandra Kukulska
Daria Handkiewicz-Junak
Sylwia Szpak-Ulczok
Aleksandra Krawczyk
Józef Roskosz
Barbara Jarząb

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