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The combine effects of proglumide and fluorouracil on the growth of murine Colon 38 cancer cells in vitro
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Abstract
The aim of this study was to examine direct effects of proglumide (10-5-10-10 M) applied either alone or together with FU (0.25, 2.5 and 25 µg/ml) on the proliferation of murine Colon 38 cancer cells in vitro. Cell proliferation was assessed by the modified colorimetric Mosmann method. Proglumide inhibited the proliferation of Colon 38 cells at the concentrations of 10-6, 10-8 and 10-10 M. FU inhibited the proliferation of cancer cells in all studied concentrations, exerting the most profound antiproliferative effect at the concentrations of 2.5 and 25 µg/ml. Thus, the former concentration was chosen to study its interactions with proglumide. Proglumide applied together with FU exerted a synergistic effect on the inhibition of proliferation of Colon 38 cells at 10-8, 10–9, 10-10 M concentrations. The most profound inhibitory effect was observed in the group incubated with FU and 10-10 M of proglumide. The obtained results indicate a possibility of new therapeutic options for colon cancer, but further studies are needed to elucidate, if the synergistic effect of FU and proglumide occurs also in the colon cancer in humans.
Abstract
The aim of this study was to examine direct effects of proglumide (10-5-10-10 M) applied either alone or together with FU (0.25, 2.5 and 25 µg/ml) on the proliferation of murine Colon 38 cancer cells in vitro. Cell proliferation was assessed by the modified colorimetric Mosmann method. Proglumide inhibited the proliferation of Colon 38 cells at the concentrations of 10-6, 10-8 and 10-10 M. FU inhibited the proliferation of cancer cells in all studied concentrations, exerting the most profound antiproliferative effect at the concentrations of 2.5 and 25 µg/ml. Thus, the former concentration was chosen to study its interactions with proglumide. Proglumide applied together with FU exerted a synergistic effect on the inhibition of proliferation of Colon 38 cells at 10-8, 10–9, 10-10 M concentrations. The most profound inhibitory effect was observed in the group incubated with FU and 10-10 M of proglumide. The obtained results indicate a possibility of new therapeutic options for colon cancer, but further studies are needed to elucidate, if the synergistic effect of FU and proglumide occurs also in the colon cancer in humans.
Keywords
proglumide; fluorouracil; proliferation; Colon 38 cancer
Title
The combine effects of proglumide and fluorouracil on the growth of murine Colon 38 cancer cells in vitro
Journal
Issue
Article type
Original paper
Pages
933-938
Published online
2006-06-26
Page views
615
Article views/downloads
986
Bibliographic record
Endokrynol Pol 2005;56(6):933-938.
Keywords
proglumide
fluorouracil
proliferation
Colon 38 cancer
Authors
Gabriela Mełeń-Mucha
Hanna Ławnicka
Sławomir Mucha