open access

Vol 56, No 6 (2005)
Original papers
Published online: 2006-06-26
Submitted: 2013-02-15
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The combine effects of proglumide and fluorouracil on the growth of murine Colon 38 cancer cells in vitro

Gabriela Mełeń-Mucha, Hanna Ławnicka, Sławomir Mucha
Endokrynologia Polska 2005;56(6):933-938.

open access

Vol 56, No 6 (2005)
Original papers
Published online: 2006-06-26
Submitted: 2013-02-15

Abstract

The role of gastrins and their receptors in the pathogenesis of colon cancer has been discussed for many years but it still remains unresolved. Although fluorouracil (FU) remains to be reference chemotherapy for colon cancer, its efficacy is unsatisfactory. Recently, we have shown a synergistic effect of proglumide (a non-selective blocker of cholecystokinin-gastrin receptors) applied together with FU, on the proliferation and apoptosis of transplantable Colon 38 cancer cells in vivo.
The aim of this study was to examine direct effects of proglumide (10-5-10-10 M) applied either alone or together with FU (0.25, 2.5 and 25 µg/ml) on the proliferation of murine Colon 38 cancer cells in vitro. Cell proliferation was assessed by the modified colorimetric Mosmann method. Proglumide inhibited the proliferation of Colon 38 cells at the concentrations of 10-6, 10-8 and 10-10 M. FU inhibited the proliferation of cancer cells in all studied concentrations, exerting the most profound antiproliferative effect at the concentrations of 2.5 and 25 µg/ml. Thus, the former concentration was chosen to study its interactions with proglumide. Proglumide applied together with FU exerted a synergistic effect on the inhibition of proliferation of Colon 38 cells at 10-8, 10–9, 10-10 M concentrations. The most profound inhibitory effect was observed in the group incubated with FU and 10-10 M of proglumide. The obtained results indicate a possibility of new therapeutic options for colon cancer, but further studies are needed to elucidate, if the synergistic effect of FU and proglumide occurs also in the colon cancer in humans.

Abstract

The role of gastrins and their receptors in the pathogenesis of colon cancer has been discussed for many years but it still remains unresolved. Although fluorouracil (FU) remains to be reference chemotherapy for colon cancer, its efficacy is unsatisfactory. Recently, we have shown a synergistic effect of proglumide (a non-selective blocker of cholecystokinin-gastrin receptors) applied together with FU, on the proliferation and apoptosis of transplantable Colon 38 cancer cells in vivo.
The aim of this study was to examine direct effects of proglumide (10-5-10-10 M) applied either alone or together with FU (0.25, 2.5 and 25 µg/ml) on the proliferation of murine Colon 38 cancer cells in vitro. Cell proliferation was assessed by the modified colorimetric Mosmann method. Proglumide inhibited the proliferation of Colon 38 cells at the concentrations of 10-6, 10-8 and 10-10 M. FU inhibited the proliferation of cancer cells in all studied concentrations, exerting the most profound antiproliferative effect at the concentrations of 2.5 and 25 µg/ml. Thus, the former concentration was chosen to study its interactions with proglumide. Proglumide applied together with FU exerted a synergistic effect on the inhibition of proliferation of Colon 38 cells at 10-8, 10–9, 10-10 M concentrations. The most profound inhibitory effect was observed in the group incubated with FU and 10-10 M of proglumide. The obtained results indicate a possibility of new therapeutic options for colon cancer, but further studies are needed to elucidate, if the synergistic effect of FU and proglumide occurs also in the colon cancer in humans.
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Keywords

proglumide; fluorouracil; proliferation; Colon 38 cancer

About this article
Title

The combine effects of proglumide and fluorouracil on the growth of murine Colon 38 cancer cells in vitro

Journal

Endokrynologia Polska

Issue

Vol 56, No 6 (2005)

Pages

933-938

Published online

2006-06-26

Bibliographic record

Endokrynologia Polska 2005;56(6):933-938.

Keywords

proglumide
fluorouracil
proliferation
Colon 38 cancer

Authors

Gabriela Mełeń-Mucha
Hanna Ławnicka
Sławomir Mucha

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