open access

Vol 57, No 5 (2006)
Original papers
Published online: 2006-11-06
Submitted: 2013-02-15
Get Citation

The effect of weight loss on serum concentrations of nitric oxide, TNF-alfa and soluble TNF-alfa receptors

Magdalena Olszanecka-Glinianowicz, Barbara Zahorska-Markiewicz, Joanna Janowska
Endokrynologia Polska 2006;57(5):487-493.

open access

Vol 57, No 5 (2006)
Original papers
Published online: 2006-11-06
Submitted: 2013-02-15

Abstract

Introduction: The aims of the present study were to evaluate the effect of weight-loss treatment on serum concentrations of NO and TNF-α and to examine whether there is an association between TNF-system activity and serum concentrations of NO after weight loss.
Material and methods: The study group involved 43 obese women (aged 41.8 ± 11.9 years, weight 95.2 ± 15.0 kg, BMI 36.5 ± 4.6 kg/m2). The women were subjected to threemonth complex weight-loss treatment. Patients were advised to keep to a 1000-1200 kcal diet and to exercise regularly. Pharmacological treatment was not administered. Serum concentrations of nitric oxide metabolites, TNF-α and its soluble receptors (sTNFR1, sTNFR2) were measured by ELISA kits; insulin was measured by RIA and glucose, cholesterol, HDL cholesterol and triglicerydes by an enzymatic procedure before and after weight loss. Body composition was determined by impedance analysis using Bodystat.
Results: The mean weight loss during treatment was 8.3 ± 4.3 kg. The serum concentrations of TNF-α decreased significantly (p < 0.000) and both receptors sTNFR1 and sTNFR2 increased significantly (p < 0.000) after weight loss. No significant changes in serum concentrations of NO were observed after weight loss. A multiple regression analysis was performed using ΔTNF-α, ΔsTNFR1, ΔTNFR2 and ΔNO as dependent variables. A significant correlation was observed between DNO and initial plasma concentrations of TNF-α, sTNFR1 and sTNFR2.
Conclusions: This study demonstrates a decrease in serum TNF-α concentration as well as an increase in plasma concentration of both TNF receptors but does not show any change in serum concentrations of NO after weight-loss treatment in obese women. It seems that changes in TNF-system activity may be a counter-regulating mechanism, which inhibits further body mass loss. We did not observe any association between changes in TNF-system activity and serum concentrations of NO after weight loss.

Abstract

Introduction: The aims of the present study were to evaluate the effect of weight-loss treatment on serum concentrations of NO and TNF-α and to examine whether there is an association between TNF-system activity and serum concentrations of NO after weight loss.
Material and methods: The study group involved 43 obese women (aged 41.8 ± 11.9 years, weight 95.2 ± 15.0 kg, BMI 36.5 ± 4.6 kg/m2). The women were subjected to threemonth complex weight-loss treatment. Patients were advised to keep to a 1000-1200 kcal diet and to exercise regularly. Pharmacological treatment was not administered. Serum concentrations of nitric oxide metabolites, TNF-α and its soluble receptors (sTNFR1, sTNFR2) were measured by ELISA kits; insulin was measured by RIA and glucose, cholesterol, HDL cholesterol and triglicerydes by an enzymatic procedure before and after weight loss. Body composition was determined by impedance analysis using Bodystat.
Results: The mean weight loss during treatment was 8.3 ± 4.3 kg. The serum concentrations of TNF-α decreased significantly (p < 0.000) and both receptors sTNFR1 and sTNFR2 increased significantly (p < 0.000) after weight loss. No significant changes in serum concentrations of NO were observed after weight loss. A multiple regression analysis was performed using ΔTNF-α, ΔsTNFR1, ΔTNFR2 and ΔNO as dependent variables. A significant correlation was observed between DNO and initial plasma concentrations of TNF-α, sTNFR1 and sTNFR2.
Conclusions: This study demonstrates a decrease in serum TNF-α concentration as well as an increase in plasma concentration of both TNF receptors but does not show any change in serum concentrations of NO after weight-loss treatment in obese women. It seems that changes in TNF-system activity may be a counter-regulating mechanism, which inhibits further body mass loss. We did not observe any association between changes in TNF-system activity and serum concentrations of NO after weight loss.
Get Citation

Keywords

nitric oxide; TNF-α; soluble TNF receptors; weight

About this article
Title

The effect of weight loss on serum concentrations of nitric oxide, TNF-alfa and soluble TNF-alfa receptors

Journal

Endokrynologia Polska

Issue

Vol 57, No 5 (2006)

Pages

487-493

Published online

2006-11-06

Bibliographic record

Endokrynologia Polska 2006;57(5):487-493.

Keywords

nitric oxide
TNF-α
soluble TNF receptors
weight

Authors

Magdalena Olszanecka-Glinianowicz
Barbara Zahorska-Markiewicz
Joanna Janowska

Important: This website uses cookies.tanya dokter More >>

The cookies allow us to identify your computer and find out details about your last visit. They remembering whether you've visited the site before, so that you remain logged in - or to help us work out how many new website visitors we get each month. Most internet browsers accept cookies automatically, but you can change the settings of your browser to erase cookies or prevent automatic acceptance if you prefer.

Via MedicaWydawcą serwisu jest  "Via Medica sp. z o.o." sp.k., ul. Świętokrzyska 73, 80–180 Gdańsk

tel.:+48 58 320 94 94, faks:+48 58 320 94 60, e-mail:  viamedica@viamedica.pl