Vol 57, Supp. A (2006)
Original paper
Published online: 2006-09-25
SNP polymorphism of LGALS3BP gene in patients with benign and malignant thyroid tumours
Abstract
Introduction: The aim of the study was estimation of occurrence
of SNP (single nucleotide polymorphism) sites in
LGALS3BP gene in patients with benign and malignant thyroid
tumors and analysis of correlation between their frequency
and the histological type of thyroid lesions.
Material and methods: The studied group consisted of 58 patients, 24 with papillary thyroid carcinomas, 19 with nodular goiters and 15 with follicular adenomas. Control group included 180 healthy volunteers. Four different SNP polymorphisms were analysed in PCR products using the SNaPshot test, on genetic analyser ABI Prism 310; these can be found in NCBI database under rs numbers: 1803938 (a/g), 11024 (g/c), 1801463 (a/c) and 1131516 (c/t).
Results: In all patients analysis of SNP sites revealed: lack of polymorphism in a/g rs 18039380; polymorphism identical to database in g/c rs 11024 and polymorphism different from database in a/g rs 1801463 and c/g rs 131516. There were no significant differences between patients with thyroid lesions and group of healthy controls.
Conclusion: Single nucleotide polymorphism (SNP) of LGALS3BP gene (found in NCBI) database are not characteristic for papillary thyroid cancer, follicular adenomas and nodular goiter.
Material and methods: The studied group consisted of 58 patients, 24 with papillary thyroid carcinomas, 19 with nodular goiters and 15 with follicular adenomas. Control group included 180 healthy volunteers. Four different SNP polymorphisms were analysed in PCR products using the SNaPshot test, on genetic analyser ABI Prism 310; these can be found in NCBI database under rs numbers: 1803938 (a/g), 11024 (g/c), 1801463 (a/c) and 1131516 (c/t).
Results: In all patients analysis of SNP sites revealed: lack of polymorphism in a/g rs 18039380; polymorphism identical to database in g/c rs 11024 and polymorphism different from database in a/g rs 1801463 and c/g rs 131516. There were no significant differences between patients with thyroid lesions and group of healthy controls.
Conclusion: Single nucleotide polymorphism (SNP) of LGALS3BP gene (found in NCBI) database are not characteristic for papillary thyroid cancer, follicular adenomas and nodular goiter.
Keywords: single nucleotide polymorphism (SNP)LGALS3BP genenodular goiterfollicular adenomasthyroid cancer
