Vol 57, Supp. A (2006)
Original paper
Published online: 2006-09-25

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Expression of selected genes involved in transport of ions in papillary thyroid carcinoma

Monika Gałęza-Kulik, Jadwiga Żebracka, Sylwia Szpak-Ulczok, Aleksandra Krawczyk, Agnieszka Czarniecka, Aleksandra Kukulska, Elżbieta Gubała, Zoran Stojcev, Małgorzata Wiench

Abstract

Introduction: The studies of papillary thyroid cancer (PTC) gene expression profile have shown changes in expression of genes involved in transport of several ions. The aim of our study was a real-time PCR evaluation of three of them: KCNJ2, SLC4A4 and SLC34A2.
Material and methods: The analysis was carried out in PTC tumors and normal thyroid samples gained from 38 patients. Real-time quantitative PCR (Q-PCR) was performed (Taqman) with β-glucuronidase (GUS) as the reference gene.
Results: We observed 20 × increase of SLC34A2 expression in PTC. This gene encodes Na+/PO4 3- cotransporter. Considerable increase of gene expression has been shown also for KCNJ2, encoding a potassium ion channel. SLC4A4, which encodes the Na+/HCO3 2- cotransporter, exhibited a 7-fold decrease in PTC.
Conclusions: The performed study revealed that SLC34A2 gene exhibited the most distinct change in expression and may become a molecular marker of papillary thyroid cancer.

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