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Expression of selected genes involved in transport of ions in papillary thyroid carcinoma
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Abstract
Material and methods: The analysis was carried out in PTC tumors and normal thyroid samples gained from 38 patients. Real-time quantitative PCR (Q-PCR) was performed (Taqman) with β-glucuronidase (GUS) as the reference gene.
Results: We observed 20 × increase of SLC34A2 expression in PTC. This gene encodes Na+/PO4 3- cotransporter. Considerable increase of gene expression has been shown also for KCNJ2, encoding a potassium ion channel. SLC4A4, which encodes the Na+/HCO3 2- cotransporter, exhibited a 7-fold decrease in PTC.
Conclusions: The performed study revealed that SLC34A2 gene exhibited the most distinct change in expression and may become a molecular marker of papillary thyroid cancer.
Abstract
Material and methods: The analysis was carried out in PTC tumors and normal thyroid samples gained from 38 patients. Real-time quantitative PCR (Q-PCR) was performed (Taqman) with β-glucuronidase (GUS) as the reference gene.
Results: We observed 20 × increase of SLC34A2 expression in PTC. This gene encodes Na+/PO4 3- cotransporter. Considerable increase of gene expression has been shown also for KCNJ2, encoding a potassium ion channel. SLC4A4, which encodes the Na+/HCO3 2- cotransporter, exhibited a 7-fold decrease in PTC.
Conclusions: The performed study revealed that SLC34A2 gene exhibited the most distinct change in expression and may become a molecular marker of papillary thyroid cancer.
Keywords
papillary thyroid carcinoma; gene expression; realtime quantitative PCR
About this articleTitle
Expression of selected genes involved in transport of ions in papillary thyroid carcinoma
Journal
Issue
Article type
Original paper
Pages
26-31
Published online
2006-09-25
Page views
702
Article views/downloads
1510
DOI
10.5603/ep.25665
Keywords
papillary thyroid carcinoma
gene expression
realtime quantitative PCR
Authors
Monika Gałęza-Kulik
Jadwiga Żebracka
Sylwia Szpak-Ulczok
Aleksandra Krawczyk
Agnieszka Czarniecka
Aleksandra Kukulska
Elżbieta Gubała
Zoran Stojcev
Małgorzata Wiench
