open access

Vol 57, Supp. A (2006)
Original papers
Published online: 2006-09-25
Submitted: 2013-02-15
Get Citation

Expression of DPP4 gene in papillary thyroid carcinoma

Joanna Ożóg, Michał Jarząb, Agnieszka Pawlaczek, Małgorzata Oczko-Wojciechowska, Jan Włoch, Józef Roskosz, Elżbieta Gubała

open access

Vol 57, Supp. A (2006)
Original papers
Published online: 2006-09-25
Submitted: 2013-02-15

Abstract

Introduction: DPP4 gene (dipeptidyl peptidase IV) is expressed in epithelial cells of many organs and cells of immune system. There is no expression of DPP4 in normal healthy thyroid, while it is highly expressed in papillary thyroid carcinoma (PTC), as shown by gene expression profiling. In this study we validated expression of DPP4 in papillary thyroid cancer and normal thyroid tissue and evaluated its usefulness for diagnostic purposes.
Material and methods: The analysis was carried out on total RNA extracted from 102 PTCs and 77 normal thyroid fragments with use of Q-PCR reaction. Beta-glucuronidase was the reference gene.
Results: We confirmed the distinct increase of DPP4 expression in papillary thyroid carcinoma. However, the ROC (relative operating characteristic) analysis revealed that the diagnostic efficiency of DPP4 estimation is limited.
Conclusions: DPP4 is increased in papillary thyroid cancer, however, its diagnostic usefulness as a single PTC marker is doubtful.

Abstract

Introduction: DPP4 gene (dipeptidyl peptidase IV) is expressed in epithelial cells of many organs and cells of immune system. There is no expression of DPP4 in normal healthy thyroid, while it is highly expressed in papillary thyroid carcinoma (PTC), as shown by gene expression profiling. In this study we validated expression of DPP4 in papillary thyroid cancer and normal thyroid tissue and evaluated its usefulness for diagnostic purposes.
Material and methods: The analysis was carried out on total RNA extracted from 102 PTCs and 77 normal thyroid fragments with use of Q-PCR reaction. Beta-glucuronidase was the reference gene.
Results: We confirmed the distinct increase of DPP4 expression in papillary thyroid carcinoma. However, the ROC (relative operating characteristic) analysis revealed that the diagnostic efficiency of DPP4 estimation is limited.
Conclusions: DPP4 is increased in papillary thyroid cancer, however, its diagnostic usefulness as a single PTC marker is doubtful.
Get Citation

Keywords

dipeptidyl peptidase IV; DPP4; papillary thyroid carcinoma; PTC; molecular markers; Q-PCR

About this article
Title

Expression of DPP4 gene in papillary thyroid carcinoma

Journal

Endokrynologia Polska

Issue

Vol 57, Supp. A (2006)

Pages

12-17

Published online

2006-09-25

Keywords

dipeptidyl peptidase IV
DPP4
papillary thyroid carcinoma
PTC
molecular markers
Q-PCR

Authors

Joanna Ożóg
Michał Jarząb
Agnieszka Pawlaczek
Małgorzata Oczko-Wojciechowska
Jan Włoch
Józef Roskosz
Elżbieta Gubała

Important: This website uses cookies.tanya dokter More >>

The cookies allow us to identify your computer and find out details about your last visit. They remembering whether you've visited the site before, so that you remain logged in - or to help us work out how many new website visitors we get each month. Most internet browsers accept cookies automatically, but you can change the settings of your browser to erase cookies or prevent automatic acceptance if you prefer.

Via MedicaWydawcą serwisu jest  "Via Medica sp. z o.o." sp.k., ul. Świętokrzyska 73, 80–180 Gdańsk

tel.:+48 58 320 94 94, faks:+48 58 320 94 60, e-mail:  viamedica@viamedica.pl