open access
The incidence of the pituitary autoantibodies in Graves’ disease
open access
Abstract
Material and methods: Studies were performed in 32 Graves’ disease patients, 25 women (age range: 31-67 yrs, median: 49.9 ± 9.4) and 7 men (age range: 41–58 yrs, median: 51.0 ± 7.1). All patients presented signs and symptoms typical of thyrotoxicosis. The diagnosis was confirmed by laboratory tests (TSH, fT3, fT4, TSH-R antibodies). Sera of control subjects were obtained from 10 healthy blood donors, 7 women, 3 men (age range 21-45 yrs, median: 30.6 ± 7.1). Incidence of pituitary autoantibodies was assessed by polyacrylamide electrophoresis gel and western-blotting. Pituitary microsomes were obtained from human pituitary tissues by ultracentrifugation and solubilisation in 1% desoxycholic acid.
Results: In 23 sera from 32 we detected autoantibodies against pituitary microsomal antigens. 16 sera were reacting with 55 kDa antigen, 10 sera with 67 kDa, 6 sera with 60 kDa, 5 sera with 52 kDa and 4 sera with 105 kDa. It is important to note that 6 sera were reacting with 57 and 55 kDa, and 5 sera with 55, 60 and 67 kDa.
Conclusions: In sera of Graves’ disease patients autoantibodies against pituitary microsomal antigens can be frequently detected. The most frequent are antibodies against 55, 60 and 67 kDa antigens.
(Pol J Endocrinol 2007; 58 (3): 195-200)
Abstract
Material and methods: Studies were performed in 32 Graves’ disease patients, 25 women (age range: 31-67 yrs, median: 49.9 ± 9.4) and 7 men (age range: 41–58 yrs, median: 51.0 ± 7.1). All patients presented signs and symptoms typical of thyrotoxicosis. The diagnosis was confirmed by laboratory tests (TSH, fT3, fT4, TSH-R antibodies). Sera of control subjects were obtained from 10 healthy blood donors, 7 women, 3 men (age range 21-45 yrs, median: 30.6 ± 7.1). Incidence of pituitary autoantibodies was assessed by polyacrylamide electrophoresis gel and western-blotting. Pituitary microsomes were obtained from human pituitary tissues by ultracentrifugation and solubilisation in 1% desoxycholic acid.
Results: In 23 sera from 32 we detected autoantibodies against pituitary microsomal antigens. 16 sera were reacting with 55 kDa antigen, 10 sera with 67 kDa, 6 sera with 60 kDa, 5 sera with 52 kDa and 4 sera with 105 kDa. It is important to note that 6 sera were reacting with 57 and 55 kDa, and 5 sera with 55, 60 and 67 kDa.
Conclusions: In sera of Graves’ disease patients autoantibodies against pituitary microsomal antigens can be frequently detected. The most frequent are antibodies against 55, 60 and 67 kDa antigens.
(Pol J Endocrinol 2007; 58 (3): 195-200)
Keywords
Graves’ disease; pituitary autoantibodies; pituitary autoantigens
About this articleTitle
The incidence of the pituitary autoantibodies in Graves’ disease
Journal
Issue
Article type
Original paper
Pages
195-200
Published online
2007-09-19
Page views
446
Article views/downloads
1123
Bibliographic record
Endokrynol Pol 2007;58(3):195-200.
Keywords
Graves’ disease
pituitary autoantibodies
pituitary autoantigens
Authors
Paweł Gut
Jerzy Kosowicz
Katarzyna Ziemnicka
Maciej Bączyk
Joanna Sawicka
Agata Czarnywojtek
Jerzy Sowiński
