open access

Vol 58, No 3 (2007)
Original papers
Published online: 2007-09-19
Submitted: 2013-02-15
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The incidence of the pituitary autoantibodies in Graves’ disease

Paweł Gut, Jerzy Kosowicz, Katarzyna Ziemnicka, Maciej Bączyk, Joanna Sawicka, Agata Czarnywojtek, Jerzy Sowiński
Endokrynologia Polska 2007;58(3):195-200.

open access

Vol 58, No 3 (2007)
Original papers
Published online: 2007-09-19
Submitted: 2013-02-15

Abstract

Introduction: It is known that in the sera of patients with Graves, Addison and other autoimmune endocrine diseases we can detect autoantibodies against pituitary antigens. The aim of the study was evaluation of pituitary autoantibodies in Graves’ disease patients using immunoblotting methods.
Material and methods: Studies were performed in 32 Graves’ disease patients, 25 women (age range: 31-67 yrs, median: 49.9 ± 9.4) and 7 men (age range: 41–58 yrs, median: 51.0 ± 7.1). All patients presented signs and symptoms typical of thyrotoxicosis. The diagnosis was confirmed by laboratory tests (TSH, fT3, fT4, TSH-R antibodies). Sera of control subjects were obtained from 10 healthy blood donors, 7 women, 3 men (age range 21-45 yrs, median: 30.6 ± 7.1). Incidence of pituitary autoantibodies was assessed by polyacrylamide electrophoresis gel and western-blotting. Pituitary microsomes were obtained from human pituitary tissues by ultracentrifugation and solubilisation in 1% desoxycholic acid.
Results: In 23 sera from 32 we detected autoantibodies against pituitary microsomal antigens. 16 sera were reacting with 55 kDa antigen, 10 sera with 67 kDa, 6 sera with 60 kDa, 5 sera with 52 kDa and 4 sera with 105 kDa. It is important to note that 6 sera were reacting with 57 and 55 kDa, and 5 sera with 55, 60 and 67 kDa.
Conclusions: In sera of Graves’ disease patients autoantibodies against pituitary microsomal antigens can be frequently detected. The most frequent are antibodies against 55, 60 and 67 kDa antigens.
(Pol J Endocrinol 2007; 58 (3): 195-200)

Abstract

Introduction: It is known that in the sera of patients with Graves, Addison and other autoimmune endocrine diseases we can detect autoantibodies against pituitary antigens. The aim of the study was evaluation of pituitary autoantibodies in Graves’ disease patients using immunoblotting methods.
Material and methods: Studies were performed in 32 Graves’ disease patients, 25 women (age range: 31-67 yrs, median: 49.9 ± 9.4) and 7 men (age range: 41–58 yrs, median: 51.0 ± 7.1). All patients presented signs and symptoms typical of thyrotoxicosis. The diagnosis was confirmed by laboratory tests (TSH, fT3, fT4, TSH-R antibodies). Sera of control subjects were obtained from 10 healthy blood donors, 7 women, 3 men (age range 21-45 yrs, median: 30.6 ± 7.1). Incidence of pituitary autoantibodies was assessed by polyacrylamide electrophoresis gel and western-blotting. Pituitary microsomes were obtained from human pituitary tissues by ultracentrifugation and solubilisation in 1% desoxycholic acid.
Results: In 23 sera from 32 we detected autoantibodies against pituitary microsomal antigens. 16 sera were reacting with 55 kDa antigen, 10 sera with 67 kDa, 6 sera with 60 kDa, 5 sera with 52 kDa and 4 sera with 105 kDa. It is important to note that 6 sera were reacting with 57 and 55 kDa, and 5 sera with 55, 60 and 67 kDa.
Conclusions: In sera of Graves’ disease patients autoantibodies against pituitary microsomal antigens can be frequently detected. The most frequent are antibodies against 55, 60 and 67 kDa antigens.
(Pol J Endocrinol 2007; 58 (3): 195-200)
Get Citation

Keywords

Graves’ disease; pituitary autoantibodies; pituitary autoantigens

About this article
Title

The incidence of the pituitary autoantibodies in Graves’ disease

Journal

Endokrynologia Polska

Issue

Vol 58, No 3 (2007)

Pages

195-200

Published online

2007-09-19

Bibliographic record

Endokrynologia Polska 2007;58(3):195-200.

Keywords

Graves’ disease
pituitary autoantibodies
pituitary autoantigens

Authors

Paweł Gut
Jerzy Kosowicz
Katarzyna Ziemnicka
Maciej Bączyk
Joanna Sawicka
Agata Czarnywojtek
Jerzy Sowiński

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