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PPAR-γ2 Pro12Ala polymorphism in the population of obese and non-obese men of the city of Wroclaw
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Abstract
Material and methods: The study group consisted of 176 healthy men, aged 25–65 years (average 54.16 years). Polymorphisms of the PPAR-g gene (Pro12Ala, Ala12Ala, Pro12Pro) were explored using the PCR-RFLP method. Plasma glucose, insulin, total cholesterol, LDL, HDL and TG were measured using commercially available kits.
Results: The genotypic distribution of the Pro12Ala polymorphism was as follows: Pro/Ala 69.8% (n = 123), Ala/Ala 28.4% (n = 50) and Pro/Pro 1.8% (n = 3). The Pro12Ala and Ala12Ala subjects did not differ in any of the measured variables. The non-obese (BMI < 30 kg/m2, n = 117) and obese subpopulations (BMI > 30 kg/m2, n = 56) did not significantly differ in the distribution of the genotypes. In the nonobese subpopulation, the homozygous Ala12 carriers (n = 38, 32.4%) had higher systolic blood pressure, plasma triglycerides, insulin levels and HOMA-IR.
Conclusions: We conclude that despite the high frequency of the Ala allele at the PPAR-γ2 gene in our population of Polish men, the Ala12 allele does not appear to improve insulin sensitivity or have an influence on the occurrence of obesity. It remains to be explained by larger studies if this polymorphism carries any risk of the development of metabolic abnormalities in non-obese men.
Abstract
Material and methods: The study group consisted of 176 healthy men, aged 25–65 years (average 54.16 years). Polymorphisms of the PPAR-g gene (Pro12Ala, Ala12Ala, Pro12Pro) were explored using the PCR-RFLP method. Plasma glucose, insulin, total cholesterol, LDL, HDL and TG were measured using commercially available kits.
Results: The genotypic distribution of the Pro12Ala polymorphism was as follows: Pro/Ala 69.8% (n = 123), Ala/Ala 28.4% (n = 50) and Pro/Pro 1.8% (n = 3). The Pro12Ala and Ala12Ala subjects did not differ in any of the measured variables. The non-obese (BMI < 30 kg/m2, n = 117) and obese subpopulations (BMI > 30 kg/m2, n = 56) did not significantly differ in the distribution of the genotypes. In the nonobese subpopulation, the homozygous Ala12 carriers (n = 38, 32.4%) had higher systolic blood pressure, plasma triglycerides, insulin levels and HOMA-IR.
Conclusions: We conclude that despite the high frequency of the Ala allele at the PPAR-γ2 gene in our population of Polish men, the Ala12 allele does not appear to improve insulin sensitivity or have an influence on the occurrence of obesity. It remains to be explained by larger studies if this polymorphism carries any risk of the development of metabolic abnormalities in non-obese men.
Keywords
PPAR-γ2 polymorphism; Pro12Ala; metabolic syndrome
About this articleTitle
PPAR-γ2 Pro12Ala polymorphism in the population of obese and non-obese men of the city of Wroclaw
Journal
Issue
Article type
Original paper
Pages
312-315
Published online
2008-07-09
Page views
622
Article views/downloads
1339
Bibliographic record
Endokrynol Pol 2008;59(4):312-315.
Keywords
PPAR-γ2 polymorphism
Pro12Ala
metabolic syndrome
Authors
Justyna Kuliczkowska
Alicja Filus
Anna Trzmiel
Urszula Tworowska
Marek Demissie
Diana Jedrzejuk
Andrzej Milewicz
