open access

Vol 59, No 6 (2008)
Original papers
Published online: 2008-10-30
Submitted: 2013-02-15
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The incidence of the pituitary autoantibodies in Addison disease

Paweł Gut, Jerzy Kosowicz, Katarzyna Ziemnicka, Maciej Bączyk, Jerzy Sowiński
Endokrynologia Polska 2008;59(6):490-494.

open access

Vol 59, No 6 (2008)
Original papers
Published online: 2008-10-30
Submitted: 2013-02-15

Abstract


Introduction: Addison disease (primary insufficience of adrenal cortex) characterized by clinical signs and symptoms associated with deficiency of adrenal hormones. The most frequent etiopathogenesis of Addison disease is related with autoimmunization. In sera of Addison patients are detectable autoantibodies against another endocrine glands. The aim of the study was evaluation of pituitary autoantibodies in Addison disease patients using immunoblotting methods.
Material and methods: Studies were performed in 19 Addison disease patients, 16 women (age range: 28-63 yrs, median: 43.5 ± 8.9) and 3 men (age range: 18-45 yrs, median: 30.6 ± 9.8). All patients presented signs and symptoms typical of primary insufficiency of adrenal cortex. Sera of control subjects were obtained from 10 healthy blood donors, 7 women, 3 men (age range 21-45 yrs, median: 30.6 ± 7.1). Incidence of pituitary autoantibodies was assessed by polyacrylamide electrophoresis gel and western-blotting. Pituitary microsomes were obtained from human pituitary tissues by ultracentrifugation and solubilisation in 1% desoxycholic acid.
Results: In 14 sera from 19 we detected autoantibodies against pituitary microsomal antigen 67 kDa, 12 sera were recting with 60 kDa and 10 sera with 55 kDa. It is important to note that 10 sera were reacting with 67 and 55 kDa, and 9 sera with 55, 60 and 67 kDa.
Conclusions: In sera of Addison disease patients autoantibodies against pituitary microsomal antigens can be frequently detected. The most frequent are antibodies against 55, 60 and 67 kDa antigens.

Abstract


Introduction: Addison disease (primary insufficience of adrenal cortex) characterized by clinical signs and symptoms associated with deficiency of adrenal hormones. The most frequent etiopathogenesis of Addison disease is related with autoimmunization. In sera of Addison patients are detectable autoantibodies against another endocrine glands. The aim of the study was evaluation of pituitary autoantibodies in Addison disease patients using immunoblotting methods.
Material and methods: Studies were performed in 19 Addison disease patients, 16 women (age range: 28-63 yrs, median: 43.5 ± 8.9) and 3 men (age range: 18-45 yrs, median: 30.6 ± 9.8). All patients presented signs and symptoms typical of primary insufficiency of adrenal cortex. Sera of control subjects were obtained from 10 healthy blood donors, 7 women, 3 men (age range 21-45 yrs, median: 30.6 ± 7.1). Incidence of pituitary autoantibodies was assessed by polyacrylamide electrophoresis gel and western-blotting. Pituitary microsomes were obtained from human pituitary tissues by ultracentrifugation and solubilisation in 1% desoxycholic acid.
Results: In 14 sera from 19 we detected autoantibodies against pituitary microsomal antigen 67 kDa, 12 sera were recting with 60 kDa and 10 sera with 55 kDa. It is important to note that 10 sera were reacting with 67 and 55 kDa, and 9 sera with 55, 60 and 67 kDa.
Conclusions: In sera of Addison disease patients autoantibodies against pituitary microsomal antigens can be frequently detected. The most frequent are antibodies against 55, 60 and 67 kDa antigens.
Get Citation

Keywords

Addison disease; pituitary autoantibodies; pituitary autoantigens

About this article
Title

The incidence of the pituitary autoantibodies in Addison disease

Journal

Endokrynologia Polska

Issue

Vol 59, No 6 (2008)

Pages

490-494

Published online

2008-10-30

Bibliographic record

Endokrynologia Polska 2008;59(6):490-494.

Keywords

Addison disease
pituitary autoantibodies
pituitary autoantigens

Authors

Paweł Gut
Jerzy Kosowicz
Katarzyna Ziemnicka
Maciej Bączyk
Jerzy Sowiński

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