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The inhibitory influence of adiponectin on the growth of the murine endothelial cell line HECa 10 in vitro
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Abstract
Background: Adiponectin, a peptide hormone secreted from the adipose tissue, has anti-diabetic, anti-atherogenic, and anti-inflammatory properties and is also involved in the regulation of angiogenesis. However, there are discrepancies among the results of the published data regarding its pro- or anti-angiogenic properties. The aim of our study was to examine the direct effect of various adiponectin concentrations applied separately or in combination with thalidomide on the growth of the murine endothelial cell line HECa 10 in 24- and 72-hour cell cultures.
Material and methods: We used immortalized murine endothelial cell line received from endothelial cells of the mouse peripheral lymph node. The effect of adiponectin was examined at concentrations from 10-5 to 10-12M. Thalidomide was used at 10-3M concentration. The growth of HECa10 cells was assessed by the colorimetric Mosmann method.
Results: We found that adiponectin inhibited the growth of HECa 10 line at all examined concentrations in the 24-hour culture, with moderate potency. There were no dose- or time-response effects. In the 72-hour cell culture, adiponectin inhibited the growth with the same or weaker potency and we did not observe its inhibitory effect at 10-12M concentration. There was no beneficial interaction between adiponectin and thalidomide. In this study, however, thalidomide alone did not cause any inhibitory effect on this cell line.
Conclusions: The obtained data show that adiponectin inhibits endothelial cell growth and may participate in angiogenesis regulation as an endogenous antiangiogenic factor.
Abstract
Background: Adiponectin, a peptide hormone secreted from the adipose tissue, has anti-diabetic, anti-atherogenic, and anti-inflammatory properties and is also involved in the regulation of angiogenesis. However, there are discrepancies among the results of the published data regarding its pro- or anti-angiogenic properties. The aim of our study was to examine the direct effect of various adiponectin concentrations applied separately or in combination with thalidomide on the growth of the murine endothelial cell line HECa 10 in 24- and 72-hour cell cultures.
Material and methods: We used immortalized murine endothelial cell line received from endothelial cells of the mouse peripheral lymph node. The effect of adiponectin was examined at concentrations from 10-5 to 10-12M. Thalidomide was used at 10-3M concentration. The growth of HECa10 cells was assessed by the colorimetric Mosmann method.
Results: We found that adiponectin inhibited the growth of HECa 10 line at all examined concentrations in the 24-hour culture, with moderate potency. There were no dose- or time-response effects. In the 72-hour cell culture, adiponectin inhibited the growth with the same or weaker potency and we did not observe its inhibitory effect at 10-12M concentration. There was no beneficial interaction between adiponectin and thalidomide. In this study, however, thalidomide alone did not cause any inhibitory effect on this cell line.
Conclusions: The obtained data show that adiponectin inhibits endothelial cell growth and may participate in angiogenesis regulation as an endogenous antiangiogenic factor.
Keywords
adiponectin; endothelial growth; in vitro
About this articleTitle
The inhibitory influence of adiponectin on the growth of the murine endothelial cell line HECa 10 in vitro
Journal
Issue
Article type
Original paper
Pages
166-171
Published online
2009-04-27
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586
Article views/downloads
1085
Bibliographic record
Endokrynol Pol 2009;60(3):166-171.
Keywords
adiponectin
endothelial growth
in vitro
Authors
Joanna Połowinczak-Przybyłek
Gabriela Mełeń-Mucha
