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Angiotensin II as a factor modulating protein tyrosine kinase activity in two breast cancer lines — MCF-7 and MDA-MB-231
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Abstract
Material and methods: The aim of this study was to evaluate possible AngII effect on the growth of two breast cancer cell lines — hormone-dependent MCF-7 and hormone-independent MDA-MB-231. We measured tyrosine kinase activity as a potential proliferation marker. We also estimated the influence of 17b-oestradiolon AngII-induced changes.
Results: In the MDA-MB-231 line, AngII radically slowed the activity of tyrosine kinases and 17b-oestradiol only at a concentration of 10–6 M, while it enhanced the effect of angiotensin II at a concentration of 10–9 M. In MCF-7, Ang II had a strong inhibitory effect in the presence of oestradiol (10–6 M). Oestradiol alone decreased the activity of examined enzymes in both cell lines. AngII receptor type 1 was found in both studied lines, but type 2 only in MDA-MB-231.
Conclusions: Our results show that AngII can modulate tyrosine kinase activity in breast tumour cell lines.
(Pol J Endocrinol 2011; 62 (2): 151–157)
Abstract
Material and methods: The aim of this study was to evaluate possible AngII effect on the growth of two breast cancer cell lines — hormone-dependent MCF-7 and hormone-independent MDA-MB-231. We measured tyrosine kinase activity as a potential proliferation marker. We also estimated the influence of 17b-oestradiolon AngII-induced changes.
Results: In the MDA-MB-231 line, AngII radically slowed the activity of tyrosine kinases and 17b-oestradiol only at a concentration of 10–6 M, while it enhanced the effect of angiotensin II at a concentration of 10–9 M. In MCF-7, Ang II had a strong inhibitory effect in the presence of oestradiol (10–6 M). Oestradiol alone decreased the activity of examined enzymes in both cell lines. AngII receptor type 1 was found in both studied lines, but type 2 only in MDA-MB-231.
Conclusions: Our results show that AngII can modulate tyrosine kinase activity in breast tumour cell lines.
(Pol J Endocrinol 2011; 62 (2): 151–157)
Keywords
angiotensin; breast cancer; MDA-MB-231; MCF-7; 17b-oestradiol; PTKs
About this articleTitle
Angiotensin II as a factor modulating protein tyrosine kinase activity in two breast cancer lines — MCF-7 and MDA-MB-231
Journal
Issue
Article type
Original paper
Pages
151-158
Published online
2011-04-29
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596
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1974
Bibliographic record
Endokrynol Pol 2011;62(2):151-158.
Keywords
angiotensin
breast cancer
MDA-MB-231
MCF-7
17b-oestradiol
PTKs
Authors
Urszula Lewandowska
Agnieszka Lachowicz-Ochędalska
Kamila Domińska
Dominika Kaszewska
Elżbieta Rębas
