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Elements of oxidation/reduction balance in experimental hypothyroidism
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Abstract
Material and methods: Investigations involved 20 Belgian giant rabbits of both sexes. Hypothyroidism was induced by intragastric administration of thiamizole. Before this was done, blood was collected from the ear marginal vein (control group) and then the animals received thiamizole through an intragastric tube at a dose of 2 mg/kg b.w. for 21 days. Blood was collected again (the experimental group) and the following determinations were performed:
— in blood serum, the thyroid hormones T3, T4 and TSH;
— vitamin A, C and E blood serum concentrations;
— in erythrocytes, the concentration of compounds reacting with TBARS, SOD and GSH-Px.
Results: A 21-day exposure of rabbits to thiamazole (2 mg/kg b.w./24 h) resulted in a statistically significant decrease of TBARS, a decrease of SOD and GPH-Px activity and in a statistically insignificant decrease in the level of vitamins A, C and E.
Conclusions: Hypothyroidism decreases the level of erythrocytes oxidation/reduction balance by diminishing oxidative lipids damage and by decreasing the activity of antioxidative enzymes, but not by changes in the level of antioxidant vitamins. (Pol J Endocrinol 2011; 62 (3): 220–223)
Abstract
Material and methods: Investigations involved 20 Belgian giant rabbits of both sexes. Hypothyroidism was induced by intragastric administration of thiamizole. Before this was done, blood was collected from the ear marginal vein (control group) and then the animals received thiamizole through an intragastric tube at a dose of 2 mg/kg b.w. for 21 days. Blood was collected again (the experimental group) and the following determinations were performed:
— in blood serum, the thyroid hormones T3, T4 and TSH;
— vitamin A, C and E blood serum concentrations;
— in erythrocytes, the concentration of compounds reacting with TBARS, SOD and GSH-Px.
Results: A 21-day exposure of rabbits to thiamazole (2 mg/kg b.w./24 h) resulted in a statistically significant decrease of TBARS, a decrease of SOD and GPH-Px activity and in a statistically insignificant decrease in the level of vitamins A, C and E.
Conclusions: Hypothyroidism decreases the level of erythrocytes oxidation/reduction balance by diminishing oxidative lipids damage and by decreasing the activity of antioxidative enzymes, but not by changes in the level of antioxidant vitamins. (Pol J Endocrinol 2011; 62 (3): 220–223)
Keywords
reactive oxygen species; antioxidants; hypothyroidism
About this articleTitle
Elements of oxidation/reduction balance in experimental hypothyroidism
Journal
Issue
Article type
Original paper
Pages
220-223
Published online
2011-06-29
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627
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1199
Bibliographic record
Endokrynol Pol 2011;62(3):220-223.
Keywords
reactive oxygen species
antioxidants
hypothyroidism
Authors
Edward Kowalczyk
Jacek Urbanowicz
Maria Kopff
Julita Ciećwierz
Grzegorz Andryskowski
