open access

Vol 63, No 2 (2012)
Original papers
Published online: 2012-04-27
Submitted: 2013-02-15
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Adenosine receptors expression is elevated in leukocytes of gestational diabetes mellitus (GDM) subjects — a preliminary study

Andrzej Zieleniak, Katarzyna Cypryk, Lucyna Alicja Wozniak, Małgorzata Bieńkiewicz, Marzena Wójcik
Endokrynologia Polska 2012;63(2):110-114.

open access

Vol 63, No 2 (2012)
Original papers
Published online: 2012-04-27
Submitted: 2013-02-15

Abstract

Introduction: Adenosine receptors (ARs), belonging to the G-protein-coupled receptors (GPCRs), are present in the majority of human cells and tissues. Depending on their biochemical and pharmacologic properties, four subtypes of ARs (i.e. A1, A2A, A2B, and A3) have been distinguished. Currently, these receptors are attractive molecular targets for pharmacological interventions in various diseases, including diabetes. The literature published to date has shown an altered expression of ARs in several types of cells under diabetic conditions. However, there has been no publication devoted to the investigation of ARs expression in leukocytes of subjects with gestational diabetes mellitus (GDM). Therefore, this study was aimed to determine the expression level of AR subtypes in leukocytes of GDM patients and its relationship to anthropometric and biochemical parameters.
Material and methods: Gene expression of four AR subtypes in leukocytes of both healthy (n = 34) and GDM (n = 67) subjects in the third trimester of pregnancy (from 24 to 33 weeks) was investigated. Multiple regression analyses were used to assess the association between the expression level of ARs and both anthropometric and biochemical parameters.
Results: Statistically significant (p < 0.05) higher levels of A2A and A2B mRNAs were observed in leukocytes of the GDM subjects compared to the control group. There was a positive correlation of A2B mRNA level with glucose concentration at 120 min of oral glucose tolerance test (OGTT) (r = 0.24, p = 0.041).
Conclusions: Overexpression of A2BAR in leukocytes of the GDM subjects and, additionally, the existence of a relationship between its elevated expression level in these cells and abnormal values of glucose concentration at 120 min of OGTT for GDM, suggest that this subtype might be involved in the pathogenesis of GDM. (Pol J Endocrinol 2012; 63 (2): 110–114)

Abstract

Introduction: Adenosine receptors (ARs), belonging to the G-protein-coupled receptors (GPCRs), are present in the majority of human cells and tissues. Depending on their biochemical and pharmacologic properties, four subtypes of ARs (i.e. A1, A2A, A2B, and A3) have been distinguished. Currently, these receptors are attractive molecular targets for pharmacological interventions in various diseases, including diabetes. The literature published to date has shown an altered expression of ARs in several types of cells under diabetic conditions. However, there has been no publication devoted to the investigation of ARs expression in leukocytes of subjects with gestational diabetes mellitus (GDM). Therefore, this study was aimed to determine the expression level of AR subtypes in leukocytes of GDM patients and its relationship to anthropometric and biochemical parameters.
Material and methods: Gene expression of four AR subtypes in leukocytes of both healthy (n = 34) and GDM (n = 67) subjects in the third trimester of pregnancy (from 24 to 33 weeks) was investigated. Multiple regression analyses were used to assess the association between the expression level of ARs and both anthropometric and biochemical parameters.
Results: Statistically significant (p < 0.05) higher levels of A2A and A2B mRNAs were observed in leukocytes of the GDM subjects compared to the control group. There was a positive correlation of A2B mRNA level with glucose concentration at 120 min of oral glucose tolerance test (OGTT) (r = 0.24, p = 0.041).
Conclusions: Overexpression of A2BAR in leukocytes of the GDM subjects and, additionally, the existence of a relationship between its elevated expression level in these cells and abnormal values of glucose concentration at 120 min of OGTT for GDM, suggest that this subtype might be involved in the pathogenesis of GDM. (Pol J Endocrinol 2012; 63 (2): 110–114)
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Keywords

gestational diabetes mellitus; GDM; adenosine receptors; gene expression

About this article
Title

Adenosine receptors expression is elevated in leukocytes of gestational diabetes mellitus (GDM) subjects — a preliminary study

Journal

Endokrynologia Polska

Issue

Vol 63, No 2 (2012)

Pages

110-114

Published online

2012-04-27

Bibliographic record

Endokrynologia Polska 2012;63(2):110-114.

Keywords

gestational diabetes mellitus
GDM
adenosine receptors
gene expression

Authors

Andrzej Zieleniak
Katarzyna Cypryk
Lucyna Alicja Wozniak
Małgorzata Bieńkiewicz
Marzena Wójcik

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