Vol 6, No 3 (2021)
Research paper
Published online: 2021-08-09

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Retrospective evaluation of laboratory findings of suspected paediatric COVID-19 patients with positive and negative RT-PCR

Eda Yaman1, Bulut Demirel2, Abdurrahman Yilmaz1, Sema Avci1, Lukasz Szarpak3
Disaster Emerg Med J 2021;6(3):97-103.


INTRODUCTION: It was observed that the new coronavirus disease had a different clinical course in children compared to adults. Fewer cases and deaths have been reported in children.  

MATERIAL AND METHODS: All children with suspected COVID-19 who applied to a secondary health care centre were included in this study and blood parameters were compared according to Reverse Transcriptase Polymerase Chain Reaction (RT-PCR) results. This study is a retrospective, cross-sectional study and was conducted by accessing the hospital records of 40 paediatric patients aged 0–16 years. RT-PCR test was performed by isolating Viral RNA with RNA Isolation System after oropharyngeal and nasopharyngeal sam­ples were taken from patients with appropriate sterile swabs and transferred to tubes containing SNP Viral Inactivation Solution. All statistical calculations were done with SPSS 23.0 (SPSS for Windows, Chicago, IL, SA). Mean values of continuous variables were compared between groups using the Mann-Whitney U test.  

RESULTS: The results were evaluated as 95% confidence interval and p-value < 0.05 and were considered statistically significant. In the light of the blood results of a small number of paediatric cases who applied, the authors tried to determine the parameters that could increase the success in diagnosis in asymptomatic paediatric cases. C-Reactive Protein, ID-dimer (quantitative), leukocytes, monocytes, sodium, mean platelet volume levels were found to be significantly different between the patients with positive and negative COVID test results.  

CONCLUSIONS: The results of this study reveal that the use of leukocytopenia, monocytopenia and mean platelet volume elevation as diagnostic markers may increase diagnostic success, especially in asymptomatic paediatric COVID-19 patients.

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  1. Li Q, Guan X, Wu P, et al. Early Transmission Dynamics in Wuhan, China, of Novel Coronavirus-Infected Pneumonia. N Engl J Med. 2020; 382(13): 1199–1207.
  2. Huang C, Wang Y, Li X, et al. Clinical features of patients infected with 2019 novel coronavirus in Wuhan, China. Lancet. 2020; 395(10223): 497–506.
  3. Malpass D. Remarks at the World Health Organization Media Briefing on COVID-19 and Vaccine Equity. 2021.
  4. Dawson S. Novel Coronavirus Disease COVID-19. 2020.
  5. Stockman LJ, Massoudi MS, Helfand R, et al. Severe acute respiratory syndrome in children. Pediatr Infect Dis J. 2007; 26(1): 68–74.
  6. Al-Tawfiq JA, Kattan RF, Memish ZA, et al. Middle East respiratory syndrome coronavirus disease in children. Pediatr Infect Dis J. 2014; 33(9): 904–906.
  7. Thabet F, Chehab M, Bafaqih H, et al. Middle East respiratory syndrome coronavirus in children. Saudi Med J. 2015; 36(4): 484–486.
  8. Chan JFW, Yuan S, Kok KH, et al. A familial cluster of pneumonia associated with the 2019 novel coronavirus indicating person-to-person transmission: a study of a family cluster. Lancet. 2020; 395(10223): 514–523.
  9. Chen ZM, Fu JF, Shu Q, et al. Diagnosis and treatment recommendations for pediatric respiratory infection caused by the 2019 novel coronavirus. World J Pediatr. 2020; 16(3): 240–246.
  10. Jiehao C, Jin Xu, Daojiong L, et al. A Case Series of Children With 2019 Novel Coronavirus Infection: Clinical and Epidemiological Features. Clin Infect Dis. 2020; 71(6): 1547–1551.
  11. Zeng LK, Tao XW, Yuan WH, et al. China’s first neonatal coronavirus pneumonia]. Zhonghua Er Ke Za Zhi = Chinese journal of pediatrics. 2020; 58: E009.
  12. Zhang YH, Lin DJ, Xiao MF, et al. [2019-novel coronavirus infection in a three-month-old baby]. Zhonghua Er Ke Za Zhi. 2020 [Epub ahead of print]; 58(0): E006.
  13. Liu Y, Yang Y, Zhang C, et al. Clinical and biochemical indexes from 2019-nCoV infected patients linked to viral loads and lung injury. Sci China Life Sci. 2020; 63(3): 364–374.
  14. Liu A, Wei J, Xu Y, et al. Report of a COVID-19 Case Combined with HIV Infection. .
  15. Cai JH, Wang XS, Ge YL, et al. [First case of 2019 novel coronavirus infection in children in Shanghai]. Zhonghua Er Ke Za Zhi. 2020 [Epub ahead of print]; 58(0): E002–87.
  16. Chen F, Liu ZS, Zhang FR, et al. [First case of severe childhood novel coronavirus pneumonia in China]. Zhonghua Er Ke Za Zhi. 2020 [Epub ahead of print]; 58(0): E005–182.
  17. Zhang GX, Zhang AM, Huang Li, et al. [Twin girls infected with SARS-CoV-2]. Zhongguo Dang Dai Er Ke Za Zhi. 2020; 22(3): 221–225.
  18. Wang D, Ju XL, Xie F, et al. [Clinical analysis of 31 cases of 2019 novel coronavirus infection in children from six provinces (autonomous region) of northern China]. Zhonghua Er Ke Za Zhi. 2020; 58(4): 269–274.
  19. Feng K, Yun YX, Wang XF, et al. [Analysis of CT features of 15 Children with 2019 novel coronavirus infection]. Zhonghua Er Ke Za Zhi. 2020 [Epub ahead of print]; 58(0): E007–278.
  20. Kam KQ, Yung CFu, Cui L, et al. A Well Infant With Coronavirus Disease 2019 With High Viral Load. Clin Infect Dis. 2020; 71(15): 847–849.
  21. Gumus H, Demir A, Yükkaldıran A. Is mean platelet volume a predictive marker for the diagnosis of COVID-19 in children? Int J Clin Pract. 2021; 75(4): e13892.
  22. Kari JA, Shalaby MA, Albanna AS, et al. Coronavirus disease in children: A multicentre study from the Kingdom of Saudi Arabia. J Infect Public Health. 2021; 14(4): 543–549.

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