Vol 5, No 1 (2020)
Research paper
Published online: 2020-01-13

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Assessment of drug susceptibility and biofilm formation ability by clinical strains of Listeria monocytogenes

Krzysztof Skowron1, Klaudia Brożek1, Magdalena Łukasik1, Natalia Wiktorczyk1, Jakub Korkus2, Eugenia Gospodarek-Komkowska1
Disaster Emerg Med J 2020;5(1):12-18.

Abstract

BACKGROUND: Listeria monocytogenes is a cause of listeriosis, dangerous especially for elderly, immunocompromised people and pregnant women. Ability to colonize biotic and abiotic surfaces and form biofilm by these pathogens poses a serious threat for the hospitalized, catheterized patients.

METHODS: The study was conducted on 29 L. monocytogenes strains isolated from clinical materials (blood, cerebrospinal fluid, swabs from vagina) and the reference strain L. monocytogenes ATCC 1911. Ability to form biofilm in 96-well plates and drug susceptibility (disk diffusion method) of tested strains was determined.

RESULTS: All strains formed biofilm though it’s intensity was correlated with source of isolation. The strong biofilm formed 72.73 % of isolates from cerebrospinal fluid ((A570 0.421 – 1.3), 75.0 % of blood isolates 9 (A570 0.389 – 1.063) and 50.0 % of isolates from vaginal swabs (A570 0.457 – 0.487). The strongest biofilm was formed by strains derived from cerebrospinal fluid whereas isolates from vaginal swabs, which strongly formed a biofilm accounted for 50.0% of the studied population (absorbance 0.457 - 0.487). It was found that 93.1 % (n=27) of strains were susceptible to all drugs tested. Two strains (6.9 %) were resistant to cotrimoxazol and 1 strain (3.45 %) to erythromycin.

CONCLUSIONS: Diverse ability to form biofilm by clinical L. monocytogenes strains is an important aspect in prophylaxis in catheterized patients.

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References

  1. Muskalska KB, Szymczak B. Postępy badań nad bakteriami rodzaju Listeria. Post Mikrobiol. 2015; 54(2): 123–132.
  2. Vázquez-Boland JA, Kuhn M, Berche P, et al. Listeria pathogenesis and molecular virulence determinants. Clin Microbiol Rev. 2001; 14(3): 584–640.
  3. Godziszewska S, Musioł E, Duda I. Listeriosis – a dangerous, contagious disease. Meningitis caused by Listeria monocytogenes – case report. Annales Academiae Medicae Silesiensis. 2015; 69: 118–124.
  4. Allerberger F, Wagner M. Listeriosis: a resurgent foodborne infection. Clin Microbiol Infect. 2010; 16(1): 16–23.
  5. Pelegrín I, Moragas M, Suárez C, et al. Listeria monocytogenes meningoencephalitis in adults: analysis of factors related to unfavourable outcome. Infection. 2014; 42(5): 817–827.
  6. Pokrowiecki R, Tyski S, Zaleska M. Problematyka Zakażeń Okołowszczepowych. Post Mikrobiol. 2014; 53: 123–134.
  7. Mah TF, O'Toole GA. Mechanisms of biofilm resistance to antimicrobial agents. Trends Microbiol. 2001; 9(1): 34–39.
  8. Anderson GG. O’Toole G.A.: Innate and induced resistance mechanisms of bacterial biofilms. Bacterial Biofilms red. Romeo T. Springer Berlin Heidelberg. 2008: 85–105.
  9. Bagge N, Schuster M, Hentzer M, et al. Pseudomonas aeruginosa biofilms exposed to imipenem exhibit changes in global gene expression and beta-lactamase and alginate production. Antimicrob Agents Chemother. 2004; 48(4): 1175–1187.
  10. Hoffman LR, D'Argenio DA, MacCoss MJ, et al. Aminoglycoside antibiotics induce bacterial biofilm formation. Nature. 2005; 436(7054): 1171–1175.
  11. de la Fuente-Núñez C, Reffuveille F, Fernández L, et al. Bacterial biofilm development as a multicellular adaptation: antibiotic resistance and new therapeutic strategies. Curr Opin Microbiol. 2013; 16(5): 580–589.
  12. European Committee on Antimicrobial Susceptibility Testing: Breakpoints tables for interpretation of MICs and zones diameters. 2018. Version 8.0. http://www.eucast.org.
  13. Holch A, Webb K, Lukjancenko O, et al. Genome sequencing identifies two nearly unchanged strains of persistent Listeria monocytogenes isolated at two different fish processing plants sampled 6 years apart. Appl Environ Microbiol. 2013; 79(9): 2944–2951.
  14. Nowak J, Cruz CD, Tempelaars M, et al. Persistent Listeria monocytogenes strains isolated from mussel production facilities form more biofilm but are not linked to specific genetic markers. Int J Food Microbiol. 2017; 256: 45–53.
  15. Di Bonaventura G, Piccolomini R, Paludi D, et al. Influence of temperature on biofilm formation by Listeria monocytogenes on various food-contact surfaces: relationship with motility and cell surface hydrophobicity. J Appl Microbiol. 2008; 104(6): 1552–1561.
  16. Tomicic R, Cabarkapa I, Vukmirovic D, et al. Influence of growth conditions on biofilm formation of Listeria monocytogenes. Food and Feed Research. 2016; 43(1): 19–24.
  17. Raby S. Porównanie zdolności tworzenia biofilmu przez szczepy Listeria monocytogenes izolowane z zakażeń i żywności. Master’s Thesis. 2016.
  18. Borges SF, Silva JGL, Teixeira PCM. Survival and biofilm formation of Listeria monocytogenes in simulated vaginal fluid: influence of pH and strain origin. FEMS Immunol Med Microbiol. 2011; 62(3): 315–320.
  19. Cirkovic I, Bozic DD, Draganic V, et al. Licheniocin 50.2 and Bacteriocins from Lactococcus lactis subsp. lactis biovar. diacetylactis BGBU1-4 Inhibit Biofilms of Coagulase Negative Staphylococci and Listeria monocytogenes Clinical Isolates. PLoS One. 2016; 11(12): e0167995.
  20. Barbosa J, Borges S, Camilo R, et al. Biofilm Formation among Clinical and Food Isolates of Listeria monocytogenes. Int J Microbiol. 2013; 2013: 524975.
  21. Doijad SP, Barbuddhe SB, Garg S, et al. Biofilm-Forming Abilities of Listeria monocytogenes Serotypes Isolated from Different Sources. 2015.
  22. Winiarska K. Porównanie częstości występowania genów kodujących wybrane czynniki wirulencji szczepów Listeria monocytogenes izolowanych z przypadków klinicznych i żywności. Master’s Thesis. 2017.
  23. Borcan AM, Huhulescu S, Munteanu A, et al. Listeria monocytogenes - characterization of strains isolated from clinical severe cases. J Med Life. 2014; 7 Spec No. 2: 42–48.
  24. Madeo M, Musumeci R, Careddu AM, et al. Antimicrobial susceptibility of Listeria monocytogenes isolates from human cases in northern Italy, 2008-2010: MIC determination according to EUCAST broth microdilution method. J Chemother. 2015; 27(4): 201–206.
  25. Caplan M, Mateescu L, Dimov T, et al. Antibiotic Susceptibility Profiles of Listeria monocytogenes Strains Isolated from Food Products and Clinical Samples. Romanian Review of Laboratory Medicine. 2014; 22(2).



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